Real-time monitoring of vancomycin using split-aptamer surface plasmon resonance biosensor DOI Creative Commons

Cátia Santa,

Soo-Hyun Park,

Artur Gejt

et al.

The Analyst, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The split-aptamer assay exploits the balance between aptamer affinity and structure for real-time monitoring of vancomycin on SPR. Further optimization improved sensitivity, while retaining repeatability under physiologically relevant conditions.

Language: Английский

Molecularly Responsive Aptamer-Functionalized Hydrogel for Continuous Plasmonic Biomonitoring DOI Creative Commons
Soo-Hyun Park, Andreas Gerber,

Cátia Santa

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Continuous in vivo monitoring of small molecule biomarkers requires biosensors with reversibility, sensitivity physiologically relevant ranges, and biological stability. Leveraging the real-time, label-free detection capability surface plasmon resonance (SPR) technology, a molecularly responsive hydrogel film is introduced to enhance sensitivity. This advanced biosensing platform utilizes split-aptamer-cross-linked hydrogels (aptagels) engineered using 8-arm poly(ethylene glycol) macromers, capable directly reversibly detecting vancomycin. Investigation through SPR optical waveguide mode, along quartz crystal microbalance dissipation (QCM-D) monitoring, reveals that reversible formation analyte-induced ternary molecular complexes leads aptagel contraction significant refractive index changes. Optimization aptamer cross-link distribution complementarity split-aptamer pairs maximizes conformational changes aptagel, demonstrating limit 160–250 nM for vancomycin (6–9 fold improvement over monolayer counterpart) broad linear sensing range up 1 mM. The maintains stability 24 h blood serum 5 weeks diluted plasma (mimicking interstitial fluid). structurally superior offers promising avenues continuous molecules.

Language: Английский

Citations

0
Efficient selection of vancomycin-specific aptamers via particle display and development of a high-sensitivity fluorescent apta-sensor for vancomycin detection DOI
Xiaona Fang, Tian Gao, Zhaofeng Luo

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137681 - 137681

Published: March 1, 2025

Language: Английский

Citations

0
Ligand‐Induced Folding in a Dopamine‐Binding DNA Aptamer DOI Creative Commons

Yunus A. Kaiyum,

Emily Hoi Pui Chao,

Lakshmi Dhar

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

Aptamers are often employed as molecular recognition elements in the development of different types biosensors. Many these biosensors take advantage aptamer having a ligand-induced structure-formation binding mechanism. However, this mechanism is poorly understood. Here we use isothermal titration calorimetry, circular dichroism spectroscopy and NMR to study structural change exhibited by dopamine-binding DNA aptamer. We analysed series aptamers where shorten terminal stem that contains 5' 3' termini sequence. All bind dopamine an enthalpically driven process coupled with unfavorable entropy. A general trend weaker affinity observed shortened. For all studied, numerous signals appear imino region

Language: Английский

Citations

3
“Binding” or “Binding and Switching”? A Perspective on Resolving Conformational Changes of Surface-Attached Biomolecular Receptors DOI

Lisa Deflandre,

Philippe Dauphin‐Ducharme

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(11), P. 5615 - 5625

Published: Oct. 24, 2024

Understanding the structural reconfiguration of a biomolecular receptor remains topic particular interest to biosensing community. This is because conformationally changing receptors are commonly employed in biosensors harness their capability bind specifically target. Often, such attached surfaces so that binding can be transduced into measurable response. Doing so, however, impose constraints on possible configurations they adopt. Such ultimately influence, for example, receptor-target models or affinity, which essential provide desired analytical performances biosensors. Motivated by idea gaining further insights impact surface attachment switching surfaces, we explore here various surface-based techniques capable monitoring changes. We decided narrow our survey have been applied investigation nucleic acids an overview key features. envision this will bring broader perspective field and challenges ahead with hopes "finding switch" surface-attached receptors.

Language: Английский

Citations

1
Real-time monitoring of vancomycin using split-aptamer surface plasmon resonance biosensor DOI Creative Commons

Cátia Santa,

Soo-Hyun Park,

Artur Gejt

et al.

The Analyst, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The split-aptamer assay exploits the balance between aptamer affinity and structure for real-time monitoring of vancomycin on SPR. Further optimization improved sensitivity, while retaining repeatability under physiologically relevant conditions.

Language: Английский

Citations

1