Bidirectionally Favorable Platform: A Dual-Targeting Probe-Encoded Maple Leaf-Type Fluorescent Lateral Flow Immunoassay for Multiple Biomarker Detection DOI
Shen Zeng,

Wanchao Zuo,

Huilin Zhang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

In the traditional multiplexed lateral flow immunoassay (LFIA), different detection probes against targets are necessary. However, relative complexity and high cost of probe preparation, as well insufficient user-friendliness, limit application LFIA in disease diagnosis. Here, we reported a bidirectionally favorable (BDF-LFIA) platform to maximize convenience for both manufacturers users. Red-emitting time-resolved fluorescent nanoparticles were coated with antibodies recognize multiple simultaneously, which greatly simplified preparation by manufacturers. Ultrabright green-emitting gold nanoclusters pre-embedded on test line reference signal achieve target concentration-dependent maple leaf-type hue readout from green yellow red, was quite user-friendly. Taking cancer biomarkers alpha-fetoprotein carcinoembryonic antigen examples, this assay achieved visual 2 ng/mL. Compared conventional LFIA, BDF-LFIA could generate more discernible around threshold concentration targets. Moreover, successfully diagnosed 54 clinical samples. Overall, showed bidirectional benefits users provided new concept detection.

Language: Английский

Bidirectionally Favorable Platform: A Dual-Targeting Probe-Encoded Maple Leaf-Type Fluorescent Lateral Flow Immunoassay for Multiple Biomarker Detection DOI
Shen Zeng,

Wanchao Zuo,

Huilin Zhang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

In the traditional multiplexed lateral flow immunoassay (LFIA), different detection probes against targets are necessary. However, relative complexity and high cost of probe preparation, as well insufficient user-friendliness, limit application LFIA in disease diagnosis. Here, we reported a bidirectionally favorable (BDF-LFIA) platform to maximize convenience for both manufacturers users. Red-emitting time-resolved fluorescent nanoparticles were coated with antibodies recognize multiple simultaneously, which greatly simplified preparation by manufacturers. Ultrabright green-emitting gold nanoclusters pre-embedded on test line reference signal achieve target concentration-dependent maple leaf-type hue readout from green yellow red, was quite user-friendly. Taking cancer biomarkers alpha-fetoprotein carcinoembryonic antigen examples, this assay achieved visual 2 ng/mL. Compared conventional LFIA, BDF-LFIA could generate more discernible around threshold concentration targets. Moreover, successfully diagnosed 54 clinical samples. Overall, showed bidirectional benefits users provided new concept detection.

Language: Английский

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