Three-Dimensional SERS-Active Hydrogel Microbeads Enable Highly Sensitive Homogeneous Phase Detection of Alkaline Phosphatase in Biosystems

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Alkaline phosphatase (ALP) is a biomarker for many diseases, and monitoring its activity level important disease diagnosis treatment. In this study, we used the microdroplet technology combined with an in situ laser-induced polymerization method to prepare Ag nanoparticle (AgNP) doped hydrogel microbeads (HMBs) adjustable pore sizes that allow small molecules enter while blocking large molecules. The AgNPs embedded microspheres can provide SERS activity, improving signal of diffuse AgNPs. A specific hydrolysis reaction ALP on 5-bromo-4-chloro-3-indolylphosphate (BCIP) was introduced itsproduct 5,5′-dibromo-4,4′-dichloro-1H,1H-[2,2']bisindolyl-3,3′-dione (BCI) employed assess due highly resonance Raman activity. sensing platform applied model serum evaluate inhibitors. assay showed higher sensitivity than UV–vis absorption spectroscopy, lowest detectable concentration 1.0 × 10–20 M. addition, HepG2 cells evaluated using platform, showing lower ALP-expressing controls response hypoxia iron metastasis. This SERS-activated HMB shows great potential detecting expected help analyze complex clinical samples.

Language: Английский

Mesoporous‐Confined Au Thorn Nano‐Pockets Enable of Precisely Capturing Target Molecules for Universal SERS Detection

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Abstract A persistent challenge in utilizing gold nanostructures for surface‐enhanced Raman scattering (SERS) lies positioning analytes into the nanoscale hotspots that maximize SERS performance and achieve universal substrates. Here, a novel mesoporous nano‐pockets strategy is proposed to design high substrates by precisely capturing target molecules plasmonic hotspots. This achieved through silica as confined growth of short Au thorns on Fe 3 O 4 core (Fe @sAT@mSiO 2 ), whilst vertically open structure with nanospace large enough at tips capture hydroxyl group surface. The electromagnetic field enhancement obtained from an average gap size nm sufficient amplify signal peaks trapped molecules. Therefore, as‐prepared proves be ultrasensitive reliable detection 15 kinds drugs, all limits lower than those reported current literature. Moreover, experimental findings are further corroborated simulations using molecular dynamics finite element method. These mesoporous‐confined thorn accessible promote future use developing method various fields.

Language: Английский

Advanced SERSome-Based Artificial-Intelligence Technology for Identifying Medicinal and Edible Homologs

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 127931 - 127931

Published: March 1, 2025

Language: Английский