Systematic trans‐Activity Comparison of Several Reported Cas12f Nucleases† DOI Open Access
Yannan Wang, Na Tang, Quanjiang Ji

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Comprehensive Summary Cas12f possesses both cis ‐ and trans ‐cleavage activities, with the former being extensively studied for its application in genome editing, while latter remains less explored, particularly diagnostic purposes, is mostly focused on Un1Cas12f1. In this study, we conducted a comprehensive comparison of activities four characterized proteins, demonstrating that all exhibit ‐DNase activity triggered by double‐stranded DNA (dsDNA), single‐stranded (ssDNA), RNA (ssRNA). Additionally, identified distinct base preferences substrates among these proteins. Our further investigation into revealed intricate relationship between under various conditions. study provides multifaceted characterization features nucleases, offering new avenues deeper comprehension mechanisms underlying Cas12f's functionality.

Language: Английский

Systematic trans‐Activity Comparison of Several Reported Cas12f Nucleases† DOI Open Access
Yannan Wang, Na Tang, Quanjiang Ji

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Comprehensive Summary Cas12f possesses both cis ‐ and trans ‐cleavage activities, with the former being extensively studied for its application in genome editing, while latter remains less explored, particularly diagnostic purposes, is mostly focused on Un1Cas12f1. In this study, we conducted a comprehensive comparison of activities four characterized proteins, demonstrating that all exhibit ‐DNase activity triggered by double‐stranded DNA (dsDNA), single‐stranded (ssDNA), RNA (ssRNA). Additionally, identified distinct base preferences substrates among these proteins. Our further investigation into revealed intricate relationship between under various conditions. study provides multifaceted characterization features nucleases, offering new avenues deeper comprehension mechanisms underlying Cas12f's functionality.

Language: Английский

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