Characterization and engineering of a plastic-degrading aromatic polyesterase

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(19)

Published: April 17, 2018

Significance Synthetic polymers are ubiquitous in the modern world but pose a global environmental problem. While plastics such as poly(ethylene terephthalate) (PET) highly versatile, their resistance to natural degradation presents serious, growing risk fauna and flora, particularly marine environments. Here, we have characterized 3D structure of newly discovered enzyme that can digest crystalline PET, primary material used manufacture single-use plastic beverage bottles, some clothing, carpets. We engineer this for improved PET capacity further demonstrate it also degrade an important replacement, polyethylene-2,5-furandicarboxylate, providing new opportunities biobased recycling.

Language: Английский

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Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis

Frontiers in Microbiology, Journal Year: 2018, Volume and Issue: 9

Published: Dec. 3, 2018

With the sharp increasing population and modernization of society, environmental pollution resulted from petroleum hydrocarbons is aggravating needs to be remediated urgently. Petroleum hydrocarbon-degrading bacteria are ubiquitous in nature can utilize these compounds as sources carbon energy. Bacteria with such functions often exploited for bioremediation oil contaminated environment. Recently, microbial remediation technology has developed rapidly theory achieved some gratifying results. However, this not omnipotent. It affected by many factors process practical application, which limits large-scale application technology. Herein, paper focuses on literatures reported usage biodegraders, discusses barriers technology, simultaneously, predicts development directions.

Language: Английский

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Biodegradation of PET: Current Status and Application Aspects

ACS Catalysis, Journal Year: 2019, Volume and Issue: 9(5), P. 4089 - 4105

Published: April 8, 2019

Most petroleum-derived plastics, as exemplified by poly(ethylene terephthalate) (PET), are chemically inactive and highly resistant to microbial attack. The accumulation of plastic waste results in environmental pollution threatens ecosystems, referred the "microplastic issue". Recently, PET hydrolytic enzymes (PHEs) have been identified we reported degradation a consortium its bacterial resident, Ideonella sakaiensis. Bioremediation may thus provide an alternative solution recycling waste. mechanism into benign monomers hydrolase mono(2-hydroxyethyl) terephthalic acid (MHET) from I. sakaiensis has elucidated; nevertheless, biodegradation require additional development for commercialization owing low catalytic activity these enzymes. Here, introduce degrading microorganisms involved, along with evolution PHEs address issues that hamper enzymatic degradation. Potential applications also discussed.

Language: Английский

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Characterization and engineering of a two-enzyme system for plastics depolymerization

Proceedings of the National Academy of Sciences, Journal Year: 2020, Volume and Issue: 117(41), P. 25476 - 25485

Published: Sept. 28, 2020

Significance Deconstruction of recalcitrant polymers, such as cellulose or chitin, is accomplished in nature by synergistic enzyme cocktails that evolved over millions years. In these systems, soluble dimeric oligomeric intermediates are typically released via interfacial biocatalysis, and additional enzymes often process the into monomers for microbial uptake. The recent discovery a two-enzyme system polyethylene terephthalate (PET) deconstruction, which employs one to convert polymer another produce constituent PET (MHETase), suggests may be evolving similar deconstruction strategies synthetic plastics. This study on characterization MHETase synergy depolymerization inform cocktail-based plastics upcycling.

Language: Английский

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Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism

Metabolic Engineering, Journal Year: 2018, Volume and Issue: 50, P. 142 - 155

Published: May 16, 2018

The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer bacterium to become flexible engineer-able platform for metabolic engineering stems its natural lifestyle, which is adapted harsh environmental conditions all sorts of physicochemical stresses. Over the years, these properties have been capitalized biotechnologically owing expanding wealth genetic tools designed deep-editing P. genome. A suite dedicated vectors inspired in core tenets synthetic biology enabled suppress many naturally-occurring undesirable traits native this species while enhancing appealing properties, also import catalytic activities attributes other biological systems. Much biotechnological interest on distinct architecture central carbon metabolism. biochemistry naturally geared generate reductive currency [i.e., NAD(P)H] that makes phenomenal host redox-intensive reactions. In some cases, editing indigenous biochemical network (cis-metabolism) has sufficed obtain target compounds industrial interest. Yet, main value promise (in particular, strain KT2440) resides not only capacity heterologous pathways microorganisms, but altogether artificial routes (trans-metabolism) making complex, new-to-Nature molecules. number examples are presented substantiating worth as one favorite workhorses sustainable manufacturing fine bulk chemicals current times 4th Industrial Revolution. potential extend rich beyond existing boundaries discussed research bottlenecks end identified. These aspects include just innovative design new strains incorporation novel chemical elements into extant biochemistry, well genomic stability scaling-up issues.

Language: Английский

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Bioremediation through microbes: systems biology and metabolic engineering approach

Critical Reviews in Biotechnology, Journal Year: 2018, Volume and Issue: 39(1), P. 79 - 98

Published: Sept. 9, 2018

Today, environmental pollution is a serious problem, and bioremediation can play an important role in cleaning contaminated sites. Remediation strategies, such as chemical physical approaches, are not enough to mitigate problems because of the continuous generation novel recalcitrant pollutants due anthropogenic activities. Bioremediation using microbes eco-friendly socially acceptable alternative conventional remediation approaches. Many with potential have been isolated characterized but, many cases, cannot completely degrade targeted pollutant or ineffective situations mixed wastes. This review envisages advances systems biology (SB), which enables analysis microbial behavior at community level under different stresses. By applying SB approach, crucial preliminary information be obtained for metabolic engineering (ME) their enhanced capabilities. also highlights integrated ME tools techniques purposes.

Language: Английский

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Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Microbial Biotechnology, Journal Year: 2018, Volume and Issue: 12(1), P. 98 - 124

Published: June 21, 2018

Summary The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful bacteria attained acceptance and widespread use are needed fulfil needs industrial bioproduction – synthesis very few, structurally simple compounds. One reasons this unfortunate circumstance has been dearth tools targeted genome engineering chassis , and, nowadays, synthetic biology is significantly helping bridge such knowledge gap. Against background, review, we discuss state art rational design construction robust engineering, presenting key examples secured place bioproduction. emergence also considered at light unique properties their physiology metabolism, practical applications which they expected outperform other microbial platforms. Emerging opportunities, essential strategies enable successful development phenotypes, major challenges field discussed, outlining solutions contemporary biology‐guided offers tackle these issues.

Language: Английский

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Recent Advanced Technologies for the Characterization of Xenobiotic-Degrading Microorganisms and Microbial Communities

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 9

Published: Feb. 10, 2021

Global environmental contamination with a complex mixture of xenobiotics has become major issue worldwide. Many xenobiotic compounds severely impact the environment due to their high toxicity, prolonged persistence, and limited biodegradability. Microbial-assisted degradation is considered be most effective beneficial approach. Microorganisms have remarkable catabolic potential, genes, enzymes, pathways implicated in process biodegradation. A number microbes, including Alcaligenes, Cellulosimicrobium, Microbacterium, Micrococcus, Methanospirillum, Aeromonas, Sphingobium, Flavobacterium, Rhodococcus, Aspergillus, Penecillium, Trichoderma, Streptomyces, Rhodotorula, Candida, Aureobasidium, been isolated characterized, shown exceptional biodegradation potential for variety contaminants from soil/water environments. potentially utilize as carbon or nitrogen sources sustain growth metabolic activities. Diverse microbial populations survive harsh contaminated environments, exhibiting significant degrade transform pollutants. However, study such requires more advanced multifaceted Currently, multiple approaches, metagenomics, proteomics, transcriptomics, metabolomics, are successfully employed characterization pollutant-degrading microorganisms, machinery, novel proteins, genes involved process. These technologies highly sophisticated, efficient obtaining information about genetic diversity community structures microorganisms. Advanced molecular used communities give an in-depth understanding structural functional aspects, help resolve issues related This review article discusses microorganisms provides insights into recent advances omics approaches specific xenobiotic-degrading

Language: Английский

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Bacterial Candidates for Colonization and Degradation of Marine Plastic Debris

Environmental Science & Technology, Journal Year: 2019, Volume and Issue: 53(20), P. 11636 - 11643

Published: Sept. 26, 2019

With the rising plastic pollution in oceans, research on plastisphere-the microorganisms interacting with marine debris-has emerged. Microbial communities colonizing have been characterized from several ocean regions and they are distinct of surrounding waters, a few plastic-degrading isolated other environments. Therefore, we propose that adapted to as surface for colonization potentially degradation. When comparing taxonomic patterns plastic-associated, bacteria, recurring groups families such Erythrobacteraceae Rhodobacteraceae (Alphaproteobacteria), Flavobacteriaceae (Bacteriodetes), phylum cyanobacteria (such Phormidium genus) can be identified. Thereby, provide perspective which bacterial candidates could play role possible degradation oceans due their occurrence debris. We emphasize need extended reproducible collection data assess existence core microbiome or functionalities plastisphere confirm capability these biodegradation plastic. Furthermore, suggest next steps elucidate level natural bioremediation exploitation degradative mechanisms

Language: Английский

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New insights into the degradation of synthetic pollutants in contaminated environments

Chemosphere, Journal Year: 2020, Volume and Issue: 268, P. 128827 - 128827

Published: Oct. 30, 2020

Language: Английский

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