Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 5, 2025
Abstract
Manipulation
of
single
cells
or
particles
is
crucial
in
the
biomedical
field.
However,
precisely
and
rapidly
manipulating
without
damaging
them
a
significant
challenge.
In
this
study,
novel
strategy
for
indirect
manipulation
microparticles
that
can
satisfy
these
requirements
via
combination
particle‐induced
dielectrophoretic
forces
(PiDEP)
optoelectronic
tweezers
(OET)
developed.
This
based
primarily
on
principle
experiencing
same
tend
to
repel
each
other,
whereas
those
different
are
attracted
other.
During
manipulation,
Ag‐SiO
2
controlled
by
OET
act
as
intermediaries
other
through
forces.
Thus,
range
be
expanded
two
three
times
its
original
size,
speed
significantly
increased
while
maintaining
precision.
Furthermore,
results
indicate
proposed
method
effectively
reduce
cell
damage
one‐third
caused
traditional
OET.
study
demonstrates
potential
particle‐assisted
single‐cell
offers
an
effective
microparticles.
Advanced Science,
Journal Year:
2021,
Volume and Issue:
9(5)
Published: Dec. 19, 2021
Over
the
past
decades,
increasing
evidence
has
indicated
that
mechanical
loads
can
regulate
morphogenesis,
proliferation,
migration,
and
apoptosis
of
living
cells.
Investigations
how
cells
sense
stimuli
or
mechanotransduction
mechanism
is
an
active
field
biomaterials
biophysics.
Gaining
a
further
understanding
regulation
depicting
network
inside
require
advanced
experimental
techniques
new
theories.
In
this
review,
fundamental
principles
various
approaches
have
been
developed
to
characterize
types
magnitudes
forces
experienced
at
cellular
subcellular
levels
are
summarized.
The
broad
applications
these
introduced
with
emphasis
on
difficulties
in
implementing
special
biological
systems.
advantages
disadvantages
each
technique
discussed,
which
guide
readers
choose
most
suitable
for
their
questions.
A
perspective
future
directions
also
provided.
It
anticipated
technical
advancement
be
driving
force
development
mechanobiology.
Small,
Journal Year:
2022,
Volume and Issue:
18(12)
Published: Jan. 29, 2022
Abstract
Cell
mechanical
forces
play
fundamental
roles
in
regulating
cellular
responses
to
environmental
stimulations.
The
shortcomings
of
conventional
methods,
including
force
resolution
and
throughput,
make
them
less
accessible
heterogeneity
at
the
single‐cell
level.
Here,
a
DNA
tensioner
platform
is
introduced
with
high
throughput
(>10
000
cells
per
chip)
pN‐level
resolution.
A
microfluidic‐based
cell
array
trapped
on
“hairpin‐structured”
tensioners
that
enable
transformation
information
living
into
fluorescence
signals.
By
using
platform,
one
can
identify
enhanced
drug‐resistant
as
compared
their
drug‐sensitive
counterparts,
differences
between
metastatic
tumor
pleural
effusion
nonmetastatic
histiocytes.
Further
genetic
analysis
traces
two
genes,
VEGFA
MINK1,
may
deterministic
heterogeneities.
In
view
ubiquity
cells’
extracellular
microenvironment
(ECM),
this
shows
wide
potential
establish
links
heterogeneity.
APL Photonics,
Journal Year:
2023,
Volume and Issue:
8(9)
Published: Sept. 1, 2023
Mechanical
metamaterials
can
adjust
mechanical
properties
of
structures
flexibly
through
a
structural
design
based
on
the
premise
that
materials
remain
unchanged.
Here,
cantilever
probe
microstructure
is
designed
using
for
an
optical
fiber
microforce
sensor
tip
be
prepared
by
femtosecond
laser-induced
two-photon
polymerization.
The
elastic
constant
k
fabricated
fiber-optic
has
been
adjusted
two
orders
magnitude
from
0.165
to
46
N/m,
and
geometric
configuration
beam
tailored
match
biological
specimens.
This
shows
ultra-high
force
sensitivity
154
nm/µN
resolution
up
130
pN.
lowest
in
direct-contact
mode
high
potential
biosensing
applications,
results
reveal
strategy
special
scanning
tunneling
microscope
probes
with
unique
physical
properties.
Reproductive Medicine and Biology,
Journal Year:
2021,
Volume and Issue:
20(4), P. 371 - 401
Published: July 31, 2021
Abstract
Background
Mechanobiology
in
the
field
of
human
female
reproduction
has
been
extremely
challenging
technically
and
ethically.
Methods
The
present
review
provides
current
knowledge
on
mechanobiology
reproductive
system.
This
focuses
early
phases
from
oocyte
development
to
embryonic
development,
with
an
emphasis
progress.
Main
findings
(Results)
Optimal,
well‐controlled
mechanical
cues
are
required
for
system
physiology.
Many
important
questions
remain
unanswered;
whether
how
imbalances
among
embryo,
decidua,
uterine
muscle
contractions
affect
biomechanical
properties
oocytes/embryos
potential
biomarkers
selecting
high‐quality
oocytes/embryos,
differ
between
two
major
compartments
ovary
(cortex
medulla)
normally
ovulating
ovaries,
durotaxis
is
involved
several
processes
addition
development.
Progress
dependent
technologies
that
enable
precise
physical
measurements.
Conclusion
More
studies
needed
understand
roles
forces
changes
Recent
future
technological
advancements
research
will
help
us
role
disorders/diseases.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 27, 2025
Cell-cell
binding,
mediated
by
the
physical
interactions
of
receptors
and
their
ligands,
plays
a
fundamental
role
in
immune
processes
such
as
surveillance
T-cell
activation.
However,
current
approaches
for
measuring
cell
avidity
often
lack
either
throughput
or
quantitative
precision.
Here,
we
introduce
high-throughput
approach
quantifying
binding
lifetimes
strength
using
centrifuge
force
microscope
(CFM)-a
compact
operating
within
standard
benchtop
centrifuge.
The
CFM
enables
live
monitoring
single-cell
under
force,
conducting
thousands
experiments
parallel.
To
facilitate
real-time
study
interactions,
developed
next-generation
with
multichannel
fluorescence
imaging
capabilities.
This
system
accommodates
measurements
two
modes:
cell-protein
cell-cell
assays.
Using
this
system,
investigated
immune-cell
Bispecific
Engager
(BiTE)
molecules,
novel
immunotherapy
designed
to
enhance
targeting
cancer
cells.
In
assays,
quantified
T-
B-cell
unbinding
from
BiTE-functionalized
surfaces,
revealing
receptor-specific
relationships
between
ligand
concentration
strength.
examined
BiTE-mediated
T-cells
Nalm6
B-cells,
precursor
leukemia
line,
uncovering
strong,
time-dependent
increase
avidity.
By
integrating
analysis,
provides
new
insights
into
dynamic
nature
immunological
broad
implications
cellular
mechanics.
Physics of Fluids,
Journal Year:
2025,
Volume and Issue:
37(3)
Published: March 1, 2025
We
present
a
contactless
method
for
measuring
the
elastic
modulus
of
living
human
triple-negative
breast
cancer
cells
(MDA-MB-231)
using
thermal
fluctuations
an
atomic
force
microscope
(AFM)
cantilever.
By
analyzing
power
spectral
density
(PSD)
cantilever's
fluctuations,
we
obtain
resonance
frequencies
its
first
three
modes
at
various
cell
to
cantilever
separation
distances.
comparing
measurements
on
with
those
rigid
borosilicate
sphere
same
size,
extract
frequency
shift
caused
by
elasto-hydrodynamic
coupling
between
and
deformations
cells.
then
fit
this
model
that
integrates
hydrodynamic
forces
deformation.
This
approach
allows
us
determine
values
resonant
Journal of Biomedical Optics,
Journal Year:
2025,
Volume and Issue:
30(03)
Published: March 14, 2025
Significance:
Measuring
cell
stiffness
is
essential
in
cellular
biomechanics,
particularly
understanding
disease
progression,
including
cancer
metastasis
and
tissue
mechanics.
However,
conventional
techniques
such
as
atomic
force
microscopy
optical
stretching
present
limitations,
invasiveness,
low
throughput,
complex
sample
preparation.
These
factors
restrict
their
applicability
dynamic
sensitive
biological
environments.Aim:
This
study
introduces
a
noninvasive
holographic
sensor
system
for
evaluating
the
of
soft
microscale
samples.Approach:
The
proposed
integrates
imaging
with
acoustic
stimulation
using
an
off-axis
Mach–Zehnder
interferometer
combined
bulk
waves.
setup
allows
label-free,
high-throughput
measurements
while
preserving
integrity.
was
validated
polyacrylamide
beads
engineered
to
mimic
stiffness,
ensuring
precise
repeatable
assessments.Results:
Measurement
errors
caused
by
spatial
variations
were
minimized
through
structured
approach
calibration
strategy,
improving
uniformity
across
different
regions.
corrections
enhanced
consistency
reliability
assessments.
Experimental
validation
demonstrated
stable
regardless
size
variations.
Repeatability
tests
further
confirmed
system's
robustness,
producing
consistent
results
multiple
trials.Conclusion:
findings
highlight
potential
this
advancing
biomechanics
research,
diagnostics,
mechanobiology.
By
offering
noninvasive,
alternative
mechanical
property
assessments
samples,
method
contributes
improved
characterization
biomedical
applications.
