Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 4, 2024

Introduction Furfural, a main inhibitor produced during pretreatment of lignocellulose, has shown inhibitory effects on S. cerevisiae . Method In the present study, new strains named 12–1 with enhanced resistance to furfural were obtained through adaptive laboratory evolution, which exhibited shortened lag phase by 36 h, and an increased ethanol conversion rate 6.67% under 4 g/L furfural. Results Discussion To further explore mechanism tolerance, ADR1_1802 mutant was constructed CRISPR/Cas9 technology, based whole genome re-sequencing data. The results indicated that time when begin grow 20 h compared reference strain ( CEN.PK113-5D) g/L. Additionally, transcription levels GRE2 ADH6 in ADR1_ 1802 53.69 44.95%, respectively, according real-time fluorescence quantitative PCR analysis. These findings suggest tolerance is due accelerated degradation. Importance: Renewable carbon worldwide vital achieve “zero carbon” target. Bioethanol from biomass one them. make bioethanol price competitive fossil fuel, higher yield necessary, therefore, monosaccharide should be effectively converted Saccharomyces However, inhibitors formed glucose or xylose oxidation could lower. Thus, tolerant important this process. As component hydrolysate, shows obvious impact growth production get find underlying mechanism, evolution technology applied study

Language: Английский