ACS Sustainable Chemistry & Engineering,
Journal Year:
2024,
Volume and Issue:
12(52), P. 18588 - 18599
Published: Dec. 18, 2024
β-Arbutin
is
a
valuable
aromatic
compound
with
multiple
biological
activities.
The
exploitation
of
efficient
engineering
microbial
platforms
for
β-arbutin
production
has
been
an
appealing
research
avenue
in
recent
years
due
to
l-tyrosine
auxotrophy
and
the
limited
distribution
metabolic
flux.
Herein,
inducer-free
nonauxotrophic
cell
factory
successfully
constructed
Escherichia
coli
toward
high-titer
by
combining
strategies.
First,
de
novo
synthesis
pathway
was
constructed,
expression
level
heterologous
enzyme
optimized
promoter
engineering.
Next,
biosynthetic
modified
rational
modular
strategies
further
enhance
production.
Subsequently,
transcription
factor
TorI
identified
negatively
regulate
biosynthesis
based
on
transcriptomic
data.
Finally,
dynamic
toggle
switch
controlling
tyrA
gene
designed
reconstruct
Without
additional
supplements
l-tyrosine,
final
strain
AT25
generated
highest
ever
reported
among
inducer-free,
strains,
producing
30.52
g/L
productivity
0.51
g/L/h.
systematic
demonstrated
this
study
can
provide
reference
establishing
factories
together
other
compounds.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(15), P. 3512 - 3512
Published: July 26, 2024
The
rational
design,
activity
prediction,
and
adaptive
application
of
biological
elements
(bio-elements)
are
crucial
research
fields
in
synthetic
biology.
Currently,
a
major
challenge
the
field
is
efficiently
designing
desired
bio-elements
accurately
predicting
their
using
vast
datasets.
advancement
artificial
intelligence
(AI)
technology
has
enabled
machine
learning
deep
algorithms
to
excel
uncovering
patterns
bio-element
data
performance.
This
review
explores
AI
design
bio-elements,
regulation
transcription-factor-based
biosensor
response
performance
AI-designed
elements.
We
discuss
advantages,
adaptability,
challenges
addressed
by
various
applications,
highlighting
powerful
potential
analyzing
data.
Furthermore,
we
propose
innovative
solutions
faced
suggest
future
directions.
By
consolidating
current
demonstrating
practical
applications
biology,
this
provides
valuable
insights
for
advancing
both
academic
biotechnology.
ACS Synthetic Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 10, 2025
The
study
of
"resource
allocator"
bacteriophage
T7
RNA
polymerase
(T7RNAP)
has
garnered
significant
interest,
particularly
for
optimizing
transcriptional
systems
in
microbial
cell
factories
(MCFs).
Most
previous
reviews
have
primarily
focused
on
T7RNAP
by
dissecting
specific
aspects
its
molecular
structure
and
functional
dynamics;
this
critical
review
seeks
to
broaden
the
scope.
We
emphasize
a
comprehensive
guide
utilizing
versatile
variants,
covering
both
fundamental
principles
fine-tuned
circuit
designs
synthetic
biology
applications.
Recent
advancements
engineered
with
enhanced
specificity
controllability
are
also
highlighted.
Furthermore,
we
discuss
host
compatibility
considerations
implementing
sustainable
bioproduction.
Finally,
key
challenges
regulatory
complexities
emerging
opportunities
next-generation
technology
discussed,
reinforcing
future
directions
improving
MCF
performance.
Applied and Environmental Microbiology,
Journal Year:
2024,
Volume and Issue:
90(11)
Published: Nov. 4, 2024
ABSTRACT
Cra
(catabolite
repressor/activator)
is
a
global
transcription
factor
(TF)
that
plays
pleiotropic
role
in
controlling
the
of
several
genes
involved
carbon
utilization
and
energy
metabolism.
Multiple
studies
have
investigated
regulatory
mechanism
its
rational
use
for
metabolic
regulation,
but
due
to
complexity
there
remain
challenges
efficient
Cra.
Here,
structure,
action,
function
nitrogen
flow
are
reviewed.
In
addition,
this
paper
highlights
application
engineering,
including
promotion
metabolite
biosynthesis,
regulation
stress
tolerance
virulence,
Cra-based
biosensor,
coupling
with
other
factors.
Finally,
prospects
Cra-related
strategies
discussed.
This
review
provides
guidance
design
construction
systems.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(45), P. 24928 - 24943
Published: Nov. 4, 2024
In
the
field
of
food
processing,
enzymes
play
a
pivotal
role
in
improving
product
quality
and
flavor,
extending
shelf
life.
However,
exposure
traditional
to
high
temperatures
during
processing
often
leads
decrease
activity
or
even
inactivation,
limiting
effectiveness
their
application
under
high-temperature
conditions.
Therefore,
modification
thermostability
adapt
extreme
conditions
through
protein
engineering
has
become
key
way
improve
efficiency
economic
benefits
industrial
production.
Directed
evolution
semirational
design
strategies
laboratory
have
proven
be
broadly
applicable
frameworks
for
biochemical
researchers
field,
including
those
who
are
beginners.
this
review,
we
systematically
summarize
high-throughput
screening
strategies,
introduce
some
intuitive
computer
simulation
software
enzyme
enzymes.
The
these
techniques
provides
comprehensive
guide
optimization
addition,
latest
hot
topics
genetically
engineered
discussed.
