Implementation of a Clostridium luticellarii genome-scale model for upgrading syngas fermentations
Computational and Structural Biotechnology Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Syngas
fermentation
is
a
powerful
platform
for
converting
waste
streams
into
sustainable
carboxylic
acid
precursors
value-added
biochemicals.
Steel
mills
produce
significant
syngas,
yet
industrial
microbial
syngas
valorization
remains
unrealized.
The
most
promising
syngas-converting
biocatalysts
consist
of
Clostridia
species,
such
as
Clostridium
kluyveri,
autoethanogenum,
and
ljungdahlii.
luticellarii,
recently
discovered
shares
close
phylogenetic
ties
with
these
organisms.
Preliminary
metabolic
studies
suggest
its
potential
acetogenesis
well
chain
elongation.
In
this
study,
we
create
iSJ444,
constraint-based
model
C.
luticellarii
using
iHN637
relative
ljungdahlii
starting
point.
Model
predictions
support
hypothesized
methanol
pathways
from
the
characterization
studies;
however,
use
propionate
could
not
be
accurately
predicted.
Thermodynamic
Flux
Analysis
(TFA)
reveals
that
maintains
stable
energy
dissipation
across
reactions
when
exposed
to
varying
pH,
increases
observed
in
associated
Wood-Ljungdahl
pathway
(WLP),
HACD1
reaction,
at
higher
pH
(6.5),
suggesting
an
adaptive
role
management
under
neutral
conditions.
sampling
simulations
exploring
flux
distributions
show
might
fit
fermenting
platforms.
both
cases,
high
hydrogen-to-carbon
source
ratios
result
better
production
(iso)butyrate
caproate.
We
present
minimal
genome-scale
foundation
further
exploration
optimization.
Although
our
behavior
await
experimental
validation,
they
underscore
enhance
Language: Английский
Enhancing methane production in two-phase anaerobic digestion of perishable organic waste: Mini-review on acidogenic fermentation pathways and regulatory strategies
Y.W. Cheng,
No information about this author
Haichuan Pan,
No information about this author
Jingmin Zhang
No information about this author
et al.
Bioresource Technology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 132253 - 132253
Published: Feb. 1, 2025
Language: Английский
Short-chain fatty acids production from maize silage: impact of stepwise hydraulic retention time reduction on microbial adaptation and process output
Deleted Journal,
Journal Year:
2025,
Volume and Issue:
2(1)
Published: March 18, 2025
Language: Английский
Kinetic Modeling of Volatile Fatty Acids Production Using Cassava Wastewater as Low-Cost Substrate
Water,
Journal Year:
2025,
Volume and Issue:
17(7), P. 991 - 991
Published: March 28, 2025
The
production
of
volatile
fatty
acids
(VFAs)
through
the
acidogenic
fermentation
wastewater
has
garnered
significant
attention
in
recent
years.
This
study
examines
kinetics
VFA
batch
reactors
using
cassava
as
a
substrate
under
previously
identified
conditions
(initial
pH
5.7,
S/M
ratio
4
gCOD/gVS,
and
temperature
34
±
1
°C).
Additionally,
this
identifies
best-fit
models
for
estimating
kinetic
parameters
related
to
consumption
soluble
organic
matter
production.
yields
ranged
from
0.15
0.44
gCODVFA/gCOD
over
12-day
period,
with
highest
yield
observed
on
day
9.
produced
consisted
29.7%
acetic
acid,
43.3%
propionic
27.0%
butyric
acid.
modified
Gompertz
first-order
residual
effectively
described
matter,
while
BPK
accurately
represented
These
showed
R2
values
lowest
RMSE
AIC
values.
Cassava
is
low-cost
potential
recovery.
Its
modeling
provides
valuable
insights
design,
control,
scale-up
reactors.
Language: Английский
Utilization strategy for algal bloom waste through co-digestion with kitchen waste: comprehensive kinetic and metagenomic analysis
Tao Huang,
No information about this author
Dong Li,
No information about this author
Bo Chen
No information about this author
et al.
Environmental Research,
Journal Year:
2024,
Volume and Issue:
255, P. 119194 - 119194
Published: May 21, 2024
Language: Английский
Biological pretreatment of organic waste for short-chain fatty acids production: State-of-the-art, advances, challenges and prospectives
Chemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
unknown, P. 157018 - 157018
Published: Oct. 1, 2024
Language: Английский
Production of Volatile Fatty Acids from Organic-Rich Waste Streams: Current Issues, Challenges, and Opportunities
Current Pollution Reports,
Journal Year:
2024,
Volume and Issue:
10(4), P. 594 - 605
Published: July 22, 2024
Language: Английский
Recovery of bio-based volatile fatty acids from anaerobically treated winery wastewater using a closed-loop liquid-liquid hydrophobic membrane contactor system
Riccardo Lo Coco,
No information about this author
Aleksandra Jelić,
No information about this author
Eliisa Järvelä
No information about this author
et al.
Chemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
500, P. 156889 - 156889
Published: Nov. 1, 2024
Language: Английский