Molecular Catalysis, Journal Year: 2024, Volume and Issue: 569, P. 114549 - 114549
Published: Sept. 17, 2024
Language: Английский
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 6, 2025
Nanobodies (Nbs), miniature antibodies consisting solely of the variable region heavy chains, exhibit unique properties such as small size, high stability, and strong specificity, making them highly promising for disease diagnosis treatment. The engineering production Nbs has evolved into a mature process, involving library construction, screening, expression purification. Different types, including immune, naïve, synthetic/semi-synthetic libraries, offer diverse options various applications, while display platforms like phage display, cell surface non-surface provide efficient screening target Nbs. Recent advancements in artificial intelligence (AI) have opened new avenues Nb engineering. AI's exceptional performance protein structure prediction molecular interaction simulation introduced novel perspectives tools design optimization. Integrating AI with traditional experimental methods is anticipated to enhance efficiency precision development, expediting transition from basic research clinical applications. This review comprehensively examines latest progress engineering, emphasizing construction strategies, platform technologies, It evaluates strengths weaknesses libraries explores potential challenges predicting structure, antigen-antibody interactions, optimizing physicochemical properties.
Language: Английский
Citations
2
Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)
Published: March 6, 2025
A variety of probiotics have been utilized as chassis strains and engineered to develop the synthetic for disease treatment. Among these probiotics, Lactobacilli, which are generally viewed safe capable colonizing gastrointestinal tract effectively, widely used. We review recent advancements in engineering Lactobacilli Specifically, that used construction application diseases treatment, therapeutic outcomes microbes summarized this review. Moreover, applications treatment categorized based on their strategies. Of note, we compare advantages disadvantages various strategies offer insights future development genetically modified Lactobacillus with stable properties. Our study comprehensively reviews researches diverse by strategies, emphasizes importance developing characteristics enhance applications.
Language: Английский
Citations
1
Environmental Science & Technology Letters, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
Management of polyethylene terephthalate (PET) plastic waste remains a challenge. PET-hydrolyzing enzymes (PHEs) such as IsPETase and variants like FAST-PETase demonstrate promising PET depolymerization capabilities at ambient temperatures can be utilized to recycle upcycle waste. Whole-cell biocatalysts displaying PHEs on their surface offer high efficiency, reusability, stability for depolymerization. However, efficacy in fully breaking down is hindered by the necessity two enzymes: PETase MHETase. Current whole-cell systems either display only one enzyme or struggle with performance when larger MHETase–PETase chimera. We developed Saccharomyces cerevisiae-based biocatalyst complete into its constituent monomers no accumulation intermediate products. Leveraging cellulosome-inspired trifunctional protein scaffoldin displayed yeast surface, we co-immobilized MHETase, forming multi-enzyme cluster. This achieved 30 °C, yielding 4.95 mM terephthalic acid (TPA) tested film. Furthermore, showed improved ability binding multiple sites scaffoldin. The whole cells had added advantage retained activity over reusability cycles. breakthrough marks step toward circular economy.
Language: Английский
Citations
0
Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132460 - 132460
Published: March 1, 2025
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0
World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)
Published: April 1, 2025
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143283 - 143283
Published: April 1, 2025
Language: Английский
Citations
0
ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 6, 2025
Escherichia coli surface display technology, which facilitates the stable of target peptides and proteins on bacterial through fusion with anchor proteins, has become a potent versatile tool in biotechnology biomedicine. The E. strategy presents unique alternative to classic intracellular extracellular expression systems, facilitating anchorage cell for functional execution. This distinctive attribute also introduces novel paradigm realm biocatalysis, harnessing cells surface-displayed enzymes catalyze conversion substrates. effectively eliminates requirement enzyme purification, overcomes limitations related substrate transmembrane transport, improves activity stability, greatly reduces cost downstream product thus making it widely used biocatalysis. Here, we review recent advances various systems technology biocatalytic applications. Additionally, discuss current this several promising methods.
Language: Английский
Citations
0
International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)
Published: March 17, 2025
Language: Английский
Citations
0
Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)
Published: April 4, 2025
Language: Английский
Citations
0