Transcriptome Analysis of Trigeminal Ganglion and Medullary Dorsal Horn in Mice to Identify Potential Targets for Pulpitis-Induced Pain DOI Creative Commons
Zhuo Yu, Xiaoning Kang,

Jialin Si

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Pulpitis is a common oral disease associated with severe pain, which often accompanied by sleep disturbances and anxiety-like symptoms. There currently no specific analgesic medication available to alleviate pulpitis-induced pain. In the current study, we applied transcriptomics conduct bioinformatics screening of trigeminal ganglion (TG) medullary dorsal horn (MDH) over 7-day period pulp exposure, aiming identify key differentially expressed genes (DEGs). We identified three DEGs in TG MDH, are primarily involved pathways related immune responses, inflammatory neuroactive ligand-receptor interactions. Potential drug candidates were pinpointed through virtual DEGs, followed molecular docking dynamics analysis. Notably, Oprm1, encodes mu-opioid receptor, exhibited significant expression differences both MDH. Further analysis C13H10Cl3N7O3S2 as most promising lead compound, suggesting that it may be an effective molecule targeting thus laying foundation for subsequent experimental validation. This finding offers new strategy pain management Oprm1 opens up pathway development novel therapeutic drugs.

Language: Английский

Transcriptome Analysis of Trigeminal Ganglion and Medullary Dorsal Horn in Mice to Identify Potential Targets for Pulpitis-Induced Pain DOI Creative Commons
Zhuo Yu, Xiaoning Kang,

Jialin Si

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Pulpitis is a common oral disease associated with severe pain, which often accompanied by sleep disturbances and anxiety-like symptoms. There currently no specific analgesic medication available to alleviate pulpitis-induced pain. In the current study, we applied transcriptomics conduct bioinformatics screening of trigeminal ganglion (TG) medullary dorsal horn (MDH) over 7-day period pulp exposure, aiming identify key differentially expressed genes (DEGs). We identified three DEGs in TG MDH, are primarily involved pathways related immune responses, inflammatory neuroactive ligand-receptor interactions. Potential drug candidates were pinpointed through virtual DEGs, followed molecular docking dynamics analysis. Notably, Oprm1, encodes mu-opioid receptor, exhibited significant expression differences both MDH. Further analysis C13H10Cl3N7O3S2 as most promising lead compound, suggesting that it may be an effective molecule targeting thus laying foundation for subsequent experimental validation. This finding offers new strategy pain management Oprm1 opens up pathway development novel therapeutic drugs.

Language: Английский

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