Food Science of Animal Resources,
Journal Year:
2024,
Volume and Issue:
45(1), P. 126 - 144
Published: Nov. 28, 2024
Rapid
population
growth
and
a
corresponding
increase
in
the
demand
for
animal-derived
proteins
have
led
to
food
supply
challenges
need
alternative
sustainable
meat
production
methods.
Therefore,
this
study
explored
importance
of
cell
engineering
technology-based
three-dimensional
bioprinting
bioinks,
which
play
key
roles
cultured
production.
In
production,
bioinks
significant
effect
on
growth,
differentiation,
mechanical
stability.
Hence,
study,
characteristics
animal-,
plant-,
marine-based
were
compared
analyzed,
impact
each
bioink
was
evaluated.
particular,
animal-based
potential
produce
that
is
similar
conventional
are
considered
most
suitable
commercialization.
Although
plant-
ecofriendly
fewer
religious
restrictions,
they
limited
terms
stability
consumer
acceptance.
further
research
required
develop
apply
optimal
commercialization
meat,
particularly
improve
its
compatibility.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Nov. 12, 2024
Scaffold
porosity
is
a
critical
factor
in
replicating
the
complex
vivo
microenvironment,
directly
influencing
cellular
interactions,
migration,
nutrient
transfer,
vascularization,
and
formation
of
functional
tissues.
For
optimal
tissue
formation,
scaffold
design
must
account
for
various
parameters,
including
material
composition,
morphology,
mechanical
properties,
compatibility.
This
review
highlights
importance
interconnected
pore
size,
emphasizing
their
impact
on
behavior
across
several
engineering
domains,
such
as
skin,
bone,
cardiovascular,
lung
Specific
size
ranges
enhance
functionality
different
tissues:
small
pores
(∼1–2
µm)
aid
epidermal
cell
attachment
skin
regeneration,
moderate
(∼2–12
support
dermal
larger
(∼40–100
facilitate
vascular
structures.
bone
engineering,
multi-layered
scaffolds
with
smaller
(50–100
foster
attachment,
while
(200–400
diffusion
angiogenesis.
Cardiovascular
tissues
benefit
from
sizes
(∼25–60
to
balance
integration
diffusion.
By
addressing
challenges
optimizing
distributions,
this
provides
insights
into
innovations,
ultimately
advancing
regeneration
strategies.
Annals of 3D Printed Medicine,
Journal Year:
2024,
Volume and Issue:
15, P. 100159 - 100159
Published: June 22, 2024
Three-dimensional
(3D)
bioprinting
technology
allows
the
production
of
porous
structures
with
complex
and
varied
geometries,
which
facilitates
development
equally
dispersed
cells
orderly
release
signal
components.
This
is
in
contrast
to
traditional
methods
used
produce
tissue
scaffolding.
To
date,
3D
has
employed
a
range
cell-laden
materials,
including
organic
synthetic
polymers,
construct
scaffolding
systems
manufacture
extracellular
matrix
(ECM).
Still,
there
are
several
challenges
meeting
technical
issues
bio-ink
formulations,
such
as
printability
bio-inks,
customization
mechanical
biological
properties
bio-implants,
guidance
cell
activities
biomaterials,
etc.
The
main
objective
this
article
discuss
various
strategies
for
preparing
bio-inks
mimic
native
tissue's
environment.
A
discussion
also
been
conducted
about
process
parameters
formulations
printing,
structure
requirements,
fabrication
durable
bio-scaffolds.
present
study
reviews
3D-printing
techniques.
Conclusively,
potential
paths
smart
bioink/scaffolds
have
outlined
regeneration.
Journal of Micromanufacturing,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 19, 2025
This
study
explores
the
fabrication
of
bone
scaffolds
using
a
composite
ink
poly-ε-caprolactone
(PCL),
polyhydroxybutyrate
(PHB)
and
synthesized
fluorapatite
(FHAp)
via
response
surface
methodology
optimization
to
achieve
optimal
Vickers
hardness
number
(VHN).
Characterization
with
X-ray
diffraction
confirms
FHAp
presence
increased
crystallinity
post-sintering,
while
Fourier-transform
infrared
spectroscopy
reveals
fundamental
material
interactions.
Results
show
PCL’s
softening
effect
at
higher
concentrations,
PHB’s
contribution
decreasing
FHAp’s
significant
role
in
reinforcing
composite.
Contour
plots
demonstrate
peak
lower
PCL
PHB
concentrations
(<11%
wt/v)
18%
wt/v
FHAp.
The
optimum
values
were
found
PCL,
9.754%
wt/v,
9.473%
24.608%
respectively,
yielding
185.34
VHN.
These
findings
offer
insights
into
optimizing
for
tailored
mechanical
properties
crucial
scaffold
design,
advancing
regenerative
medicine
tissue
engineering.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 26, 2024
Angiogenesis
is
the
formation
of
blood
vessels
from
existing
vasculature,
which
important
in
tumor
growth
where
metastatic
spread,
cancer
cells
depends
on
an
adequate
supply
oxygen
and
nutrients
removal
waste
products.
Targeting
angiogenesis
has
emerged
as
a
promising
therapeutic
strategy
for
treatment.
This
study
presents
synthesis
evaluation
novel
Ag-CeO2-ZnO
(AgCZ)
nanocomposite
designed
to
specifically
inhibit
effective
therapy.
The
was
synthesized
via
glycine-assisted
combustion
method,
its
physicochemical
properties
were
meticulously
characterized
using
advanced
analytical
techniques.
anti-angiogenesis
potential
AgCZ
vigorously
explored
through
assortment
vitro
investigations,
with
particular
interest
inhibiting
agents
like
vascular
endothelial
factor
(VEGF).
In
silico
data
molecular
docking
studies
instrumental
elucidating
nanocomposite's
primary
reported
mechanism
action,
i.e.,
strong
VEGF
target
bond.
Notably,
had
selective
cytotoxicity
different
types
no
sign
serious
influence
onto
normal
cells,
reflecting
great
promise
targeted
Not
importantly,
implemented
measure
anti-angiogenic
well
anti-tumor
effect
biological
models
additionally.
Our
highlights
emerging
developments
medicine
draws
possible
future
paths.
use
composite
nanoparticle
one
anticancer
drug
alternative
conventional
appears
be
safer
more
effectual,
but
further
research
needed
overcome
current
limitations
clinical
translation.
Polymers for Advanced Technologies,
Journal Year:
2024,
Volume and Issue:
35(12)
Published: Dec. 1, 2024
ABSTRACT
This
review
provides
a
comprehensive
overview
of
the
emerging
applications
stimuli‐responsive
hydrogels
in
3D
printing,
emphasizing
their
transformative
potential
creating
adaptive
and
multifunctional
structures.
Stimuli‐responsive
hydrogels,
including
magneto‐,
thermo‐,
pH‐,
moisture‐,
solvent‐,
photo‐responsive
varieties,
have
gained
significant
attention
due
to
ability
undergo
dynamic
changes
response
specific
environmental
stimuli.
The
begins
by
exploring
fundamental
characteristics
fabrication
methods
used
additive
manufacturing,
highlighting
exceptional
adaptability
programmability.
It
then
delves
into
various
across
diverse
fields,
soft
robotics,
tissue
engineering,
drug
delivery
systems,
wearable
electronics,
food
technology,
electromagnetic
interference
shielding,
anti‐counterfeiting
technologies.
By
integrating
latest
advancements
printing
techniques,
this
aims
offer
insights
how
are
enabling
development
innovative,
intelligent,
environmentally
responsive
systems.
future
perspectives
section
discusses
challenges
opportunities
for
advancing
use
suggesting
directions
research
that
could
push
boundaries
functional
materials
programmable
Gels,
Journal Year:
2024,
Volume and Issue:
10(6), P. 406 - 406
Published: June 18, 2024
Fabrication
of
scaffolds
via
3D
printing
is
a
promising
approach
for
tissue
engineering.
In
this
study,
we
combined
with
cryogenic
crosslinking
to
create
biocompatible
gelatin/oxidized
alginate
(Gel/OxAlg)
large
pore
sizes,
beneficial
bone
regeneration.
To
enhance
the
osteogenic
effects
and
mechanical
properties
these
scaffolds,
evaluated
impact
hydroxyapatite
(HAp)
on
rheological
characteristics
2.86%
(1:1)
Gel/OxAlg
ink.
We
investigated
morphological
low,
5%,
high
10%
HAp
content,
as
well
resulting
bio-
effects.
Scanning
electron
microscopy
revealed
reduction
in
sizes
from
160
180
µm
(HAp-free)
120
140
both
HAp-containing
scaffolds.
Increased
stability
higher
Young’s
moduli
were
measured
5%
(18
21
kPa,
respectively)
compared
11
kPa
HAp-free
constructs.
Biological
assessments
mesenchymal
stem
cells
indicated
excellent
cytocompatibility
differentiation
all
degree
mineralization
Scaffolds
exhibited
improved
shape
fidelity,
demonstrated
positive
impact,
enhanced
formation.
Increasing
content
did
not
show
any
advantages
osteogenesis,
offering
minor
increase
strength
at
cost
significantly
compromised
fidelity.
Fabrication
of
scaffolds
via
3D
printing
is
a
promising
approach
for
tissue
engineering.
In
this
study,
we
combined
with
cryogenic
crosslinking
to
create
biocompatible
gela-tin/oxidized
alginate
(Gel/OxAlg)
large
pore
sizes,
beneficial
bone
re-generation.
To
enhance
the
osteogenic
effects
and
mechanical
properties
these
scaffolds,
evaluated
impact
hydroxyapatite
(HAp)
on
rheological
characteristics
2.86%
(1:1)
Gel/OxAlg
ink.
We
investigated
morphological
low,
5%
high
10%
HAp
content,
as
well
resulting
bio
effects.
Scanning
electron
microscopy
revealed
reduction
in
sizes
from
160-180
µm
(HAp-free)
120-140
both
HAp-containing
scaffolds.
Increased
stability
higher
Young’s
moduli
were
measured
(18
21
kPa,
respectively)
compared
11
kPa
HAp-free
constructs.
Biological
assessments
mesenchymal
stem
cells
indicated
excellent
cytocompati-bility
differentiation
all
degree
mineralization
Scaffolds
exhibit
improved
shape
fidelity,
demonstrate
positive
impact,
formation.
Increasing
content
did
not
show
any
advantages
osteogenesis,
offering
minor
increase
strength
at
cost
significantly
compromised
fidelity.
Biomaterials Advances,
Journal Year:
2024,
Volume and Issue:
164, P. 213985 - 213985
Published: Aug. 10, 2024
Bone
regeneration
often
fails
due
to
implants/grafts
lacking
vascular
supply,
causing
necrotic
tissue
and
poor
integration.
Microsurgical
techniques
are
used
overcome
this
issue,
allowing
the
graft
anastomose.
These
have
limitations,
including
severe
patient
morbidity
current
research
focuses
on
stimulating
angiogenesis
in
situ
using
growth
factors,
presenting
such
as
a
lack
of
control
increased
costs.
Non-biological
stimuli
necessary
promote
for
successful
bone
constructs.
Recent
studies
reported
that
bioactive
glass
dissolution
products,
calcium-releasing
nanoparticles,
stimulate
hMSCs
new
vasculature.
Moreover,
effect
3D
microporosity
has
also
been
be
important
vascularisation
vivo.
Therefore,
we
room-temperature
extrusion
printing
with
polylactic
acid
(PLA)
calcium
phosphate
(CaP)
based
scaffolds,
focusing
geometry
solvent
displacement
scaffold
recovery.
Combining
both
methods
enabled
reproducible
structure,
porosity,
surface
topography.
Scaffolds
maintained
ion
release
at
physiological
levels
supported
human
mesenchymal
stem
cell
proliferation.
stimulated
secretion
endothelial
factor
(VEGF)
after
3
days
culture.
Subcutaneous
implantation
vivo
indicated
good
integration
blood
vessel
infiltration
early
one
week
after.
PLA-CaP
scaffolds
showed
maturation
4
weeks
without
regression.
Results
show
PLA/CaP-based
made
via
controlled
printing,
support
maturation,
promising
improved
vascularization
regeneration.
Polymers,
Journal Year:
2024,
Volume and Issue:
16(14), P. 2023 - 2023
Published: July 15, 2024
Human
bone
is
composed
of
organic
and
inorganic
composite
materials,
contributing
to
its
unique
strength
flexibility.
Hydroxyapatite
(HAP)
has
been
extensively
studied
for
regeneration,
due
excellent
bioactivity
osteoconductivity,
which
makes
it
a
highly
valuable
biomaterial
tissue
engineering
applications.
For
better
therapeutic
effects,
nanofibers
containing
polyvinyl
alcohol
(PVA)
Pyrrolidone
(PVP)
were
developed
using
an
electrospinning
technique
in
this
study.
Herein,
hydroxyapatite
(a
major
constituent
native
bone)
concentrations
varying
from
5
25%
reinforced
the
composite,
could
alter
properties
nanofibers.
The
as-prepared
characterized
by
SEM,
TEM,
XRD,
FT-IR
spectroscopy,
assessment
was
performed
simulated
body
fluid
(SBF).
ICP-OES
analysis
used
determine
concentration
Ca2+
PO42–
ions
before
after
SBF
immersion.
To
optimize
material
selection,
nanofibrous
scaffolds
subjected
cell
proliferation
differentiation
MG-63
osteoblast
lines,
but
no
significant
toxicity
observed.
In
conclusion,
HAP-PVA-PVP
exhibit
physical
chemical
ideal
biocompatibility,
with
great
promise
serve
as
effective
candidates
