Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism DOI Creative Commons
Lu Wang,

Menghan Xi,

Wei Cao

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Electroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbiota composition and metabolism in EA-treated constipated mice, along their targets, remain unidentified. We examined microbiome composition, metabolism, colonic miRNA expression loperamide-induced mice to identify differentially expressed assess relationships microbial abundance, An antibiotic cocktail adeno-associated virus were employed interfere target vivo, thereby validating proposed mechanism. Our results indicate that miR-205-5p, significantly upregulated tissues of promotes intestinal motility microbiome-dependent manner. Specifically, EA promoted growth Lactobacillus reuteri , enriched feces constipation-recovered through host-derived miR-205-5p regulation. Furthermore, its tryptophan metabolites (indole-3-acetamide, indole-3-acetic acid, indole-3-carboxaldehyde) alleviated constipation. These findings underscore pivotal role modulating EA.

Language: Английский

Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism DOI Creative Commons
Lu Wang,

Menghan Xi,

Wei Cao

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Electroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbiota composition and metabolism in EA-treated constipated mice, along their targets, remain unidentified. We examined microbiome composition, metabolism, colonic miRNA expression loperamide-induced mice to identify differentially expressed assess relationships microbial abundance, An antibiotic cocktail adeno-associated virus were employed interfere target vivo, thereby validating proposed mechanism. Our results indicate that miR-205-5p, significantly upregulated tissues of promotes intestinal motility microbiome-dependent manner. Specifically, EA promoted growth Lactobacillus reuteri , enriched feces constipation-recovered through host-derived miR-205-5p regulation. Furthermore, its tryptophan metabolites (indole-3-acetamide, indole-3-acetic acid, indole-3-carboxaldehyde) alleviated constipation. These findings underscore pivotal role modulating EA.

Language: Английский

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