Early-life Adversity-Induced Epigenetic Reprogramming of Prefrontal Cortex in Rats Subjected to Maternal Separation

Biological Psychiatry Global Open Science, Journal Year: 2025, Volume and Issue: unknown, P. 100487 - 100487

Published: March 1, 2025

Language: Английский

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Long-term Impact of Early-Life Stress on Hippocampal Apoptotic Gene Expression in Mice

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Early-life stress (ELS) such as maternal separation has been associated with neuronal apoptosis and impaired hippocampal function in rodent models. This study investigated the long-term effects of early-life unpredictable (MS) MS combined (MSUS) from postnatal days 1 to 14 on mRNA expression levels proapoptotic genes Bax, Tp53 Casp3, prosurvival gene Bcl2 hippocampus mice (in vivo). Additionally, total RNA MSUS was nucleofected at different concentrations into healthy mouse cells, followed by 3D cell culture using N-heptyl-D-galactonamide (GalC7) hydrogels scaffolds for growth apoptotic studies vitro). Quantitative real-time PCR conducted assess target genes, which were subsequently analyzed comparative Ct method (2^-ΔΔCt). Bax significantly lower group than control group, whereas both groups presented significant increases Bcl2 mRNA expression. In addition, ratio Bcl2/Bax greater groups. No differences or Casp3 detected between Although in vitro not different, Bcl2/Bax reached equilibrium concentration increased. Our results suggested that, response ELS, cells adapt prioritize survival over apoptosis.

Language: Английский

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Transforming neonatal nursing: a randomized controlled trial comparing kangaroo care and standard protocols for survival in preterm infants with respiratory distress syndrome

BMC Nursing, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 16, 2025

Language: Английский

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Peripheral CD4 ⁺ T cells mediate the destructive effects of maternal separation on prefrontal myelination and cognitive functions

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(16)

Published: April 16, 2025

Maternal separation (MS), a chronic stress event in early life, impairs myelination the prefrontal cortex (PFC) and leads to PFC cognitive disorders. It remains largely unclear how such deficits are mediated. Here, we show that peripheral CD4 + T cells play an essential role mediating destructive effects of MS on medial cortical (mPFC) functions mice. Offspring mice with experience (MS mice) exhibited increase xanthine levels blood severe deficit mPFC-dependent as working memory, social interaction, anxiety/depression emotion regulation, along decrease oligodendrocyte precursor (OPCs) oligodendrocytes (OLs) mPFC. These phenotypes were rescued upon treatment antibody neutralizing cells. Rag1 −/− immunodeficient receiving transplantation isolated from showed similar observed Immunofluorescence staining revealed rich expression adenosine receptor A1 (A1) OPCs mPFC, A1-expressing decreased cell transplantation. The present study demonstrates causal link between MS-induced hypomyelination dysfunction, is probably mediated via xanthine–adenosine signaling OPCs.

Language: Английский

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Effect of Shifting From Open Bay to Single‐Family Rooms on Closeness in a NICU

Acta Paediatrica, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

ABSTRACT Aim To assess the impact of moving from open bay units (OBU) to single‐family rooms (SFR) on parental closeness in Neonatal Intensive Care Unit (NICU). Methods This observational longitudinal cohort study was conducted between February 2021 and January 2024, during which time setting changed OBU SFR with a mixed phase between. All parent‐infant dyads were eligible for inclusion. The self‐reported present unit, lap holding, skin‐to‐skin care (SSC) used measure closeness. Results 833 parents infants included (365 OBU, 140 mixed, 328 phase). Parental presence increased mothers 210 min/day 420 ( p = 0.000) SFR, partners 180 360 < 0.001). Only mothers, holding 100 120 0.046). SSC 160 0.002) 110 130 0.004) partners. These findings independent gestational age. Conclusion Moving an NICU. However, more changes are needed meet EFCNI WHO recommendations.

Language: Английский

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Maternal exercise during pregnancy: Sex-specific impacts on offspring memory and maternal deprivation effects

Neuroscience Letters, Journal Year: 2025, Volume and Issue: unknown, P. 138252 - 138252

Published: April 1, 2025

Language: Английский

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