Developing Magnetic Resonance Imaging Biomarkers of Neuroinflammation, Cognitive Impairment, and Survival Outcomes for Radiotherapy-Induced Brain Injury in a Preclinical Mouse Model DOI

Maya Teitz,

Esteban Velarde,

Xiaochun Yang

et al.

Investigative Radiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Objective Radiotherapy-induced brain injury (RIBI) is a chronic side effect that affects up to 90% of tumor survivors treated with radiotherapy. Here, we used multiparametric magnetic resonance imaging (MRI) identify noninvasive and clinically translatable biomarkers RIBI. Method 8-week-old female, immune competent BALB/c mice were stereotactically irradiated single dose 80 Gy, at rate 1.7 Gy/minute. The then monitored longitudinally MRI, behavioral tests learning memory, immunohistochemistry, in comparison nonirradiated mice. Results Three types MRI RIBI identified. A contrast-enhanced T 1 -weighted biomarker was identified as being best suited detect the onset injury, by detecting changes blood-brain barrier (BBB) permeability. Maximum BBB permeability (18.95 ± 1.75) detected 1-month postirradiation ( P < 0.0001, n = 3). Interestingly, maximum neuroinflammation (24.14 6.72) also using IBA1 CD68 immunohistochemistry 0.0041, This simultaneous detection coincided transient cognitive impairment, fear-conditioning test compared 0.0017, 10). 2 hyperintensity identified, determined be intermediate injury. (3.97 2.07) 2-month 0.0368, correlated astrogliosis (9.92 4.21), which GFAP 0.0215, Finally, *-weighted hypointensity late from 4-month postirradiation. These increased iron deposition vascular damage, validated Perls' Prussian blue histology 0.05, hypointense preceded significant weight loss, severe decreased survival Conclusions 3 translational could enable longitudinal evaluation potential prophylactic therapeutic agents. play pivotal role management survivors.

Language: Английский

Developing Magnetic Resonance Imaging Biomarkers of Neuroinflammation, Cognitive Impairment, and Survival Outcomes for Radiotherapy-Induced Brain Injury in a Preclinical Mouse Model DOI

Maya Teitz,

Esteban Velarde,

Xiaochun Yang

et al.

Investigative Radiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Objective Radiotherapy-induced brain injury (RIBI) is a chronic side effect that affects up to 90% of tumor survivors treated with radiotherapy. Here, we used multiparametric magnetic resonance imaging (MRI) identify noninvasive and clinically translatable biomarkers RIBI. Method 8-week-old female, immune competent BALB/c mice were stereotactically irradiated single dose 80 Gy, at rate 1.7 Gy/minute. The then monitored longitudinally MRI, behavioral tests learning memory, immunohistochemistry, in comparison nonirradiated mice. Results Three types MRI RIBI identified. A contrast-enhanced T 1 -weighted biomarker was identified as being best suited detect the onset injury, by detecting changes blood-brain barrier (BBB) permeability. Maximum BBB permeability (18.95 ± 1.75) detected 1-month postirradiation ( P < 0.0001, n = 3). Interestingly, maximum neuroinflammation (24.14 6.72) also using IBA1 CD68 immunohistochemistry 0.0041, This simultaneous detection coincided transient cognitive impairment, fear-conditioning test compared 0.0017, 10). 2 hyperintensity identified, determined be intermediate injury. (3.97 2.07) 2-month 0.0368, correlated astrogliosis (9.92 4.21), which GFAP 0.0215, Finally, *-weighted hypointensity late from 4-month postirradiation. These increased iron deposition vascular damage, validated Perls' Prussian blue histology 0.05, hypointense preceded significant weight loss, severe decreased survival Conclusions 3 translational could enable longitudinal evaluation potential prophylactic therapeutic agents. play pivotal role management survivors.

Language: Английский

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