Metagenomic analysis and bioactive profiling of kombucha fermentation: antioxidant, antibacterial activities, and molecular docking insights into gastric cancer therapeutics DOI

Thakur Shalini,

Ragothaman Prathiviraj,

P. Senthilraja

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 13(6)

Published: Nov. 5, 2024

Abstract Kombucha is fermented and produced with a biofilm called symbiotic culture of bacteria yeast, which drunk all over the world for its beneficial effects on human health energy levels. The metagenomic study kombucha frequently detected microorganisms in proteobacteria, firmicutes, actinobacteria. And also, yeast fungi are Ascomycota Basidiomycota present green leaf sugarcane juice kombucha. extracts’ biological activities were assessed using pH, total phenolic content, antioxidant, antibacterial, anticancer activity. Fermentation may enhance activity generation bioactive substances. These results showed pH −3.1 ± 0.2 TPC −0.721 μg/mL gallic acid equivalent. antioxidant radicals scavenging was evaluated by DPPH, ABTS, H2O2 TAC. chemicals identified FT-IR HR-LC/MS analysis totaled 45 components. compounds further move to perform molecular docking against gastric cancer target proteins 4H9M, 2DQ7 1TVO binding Nequinate showing best LibDock scores 105.12, 114.49, 108.97. So, this suggests that knowledge can potentially active it’s stimulated mechanism gastrointestinal transit. Additionally, gives strength understand bacterial fungal distribution from Kombucha.

Language: Английский

Preparation and evaluation of Magnetospirillum gryphiswaldense MSR-1 bioinoculant on the growth and productivity of Vigna radiata (L.) R. Wilczek DOI

Shraddha Shirsat,

K. Suthindhiran

3 Biotech, Journal Year: 2025, Volume and Issue: 15(2)

Published: Jan. 21, 2025

Language: Английский

Citations

0
Nepenthes pitcher fluid for the green synthesis of silver nanoparticles with biofilm inhibition, anticancer and antioxidant properties DOI Creative Commons
Ovungal Sabira, Anthyalam Parambil Ajaykumar, Sudhir Varma

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 13, 2025

This is the first report of silver nanoparticles (AgNPs) synthesis utilizing pitcher secretion from an insectivorous plant, specifically Nepenthes ventrata, through a microwave assisted green approach. The successful formation AgNPs was validated comprehensive set analyses, including UV–Vis spectroscopy, Fourier-transform infrared transmission electron microscopy, DLS analysis and Zeta potential measurements. In addition gas chromatography-high-resolution mass spectrometry liquid analyses were conducted to examine components present in secretion. These aimed identify capping stabilizing agents that facilitate stability AgNPs. synthesized significantly inhibited biofilm by Pseudomonas aeruginosa PAO1, as demonstrated Crystal Violet staining fluorescence microscopy. Additionally, these showed promising antioxidant properties DPPH radical scavenging assay. Furthermore, anticancer analyzed using MTT assay, which measures cell metabolic activity indicator viability, proliferation, cytotoxicity. Collectively, findings suggest biosynthesized possess multifaceted biological applications, showcasing their utility both antimicrobial antioxidative agents, highlighting medical environmental applications.

Language: Английский

Citations

0
Characterization of soil-derived Bacillus subtilis metabolites against breast cancer: In vitro and in silico studies DOI Creative Commons
A. Hashmi, Mehwish Iqtedar, Hamid Saeed

et al.

Saudi Pharmaceutical Journal, Journal Year: 2025, Volume and Issue: 33(1-2)

Published: April 17, 2025

Language: Английский

Citations

0
Exploring the bioactive ingredients of three traditional Chinese medicine formulas against age-related hearing loss through network pharmacology and experimental validation DOI Creative Commons
Wenying Shi, Qi Zhao, Hongwei Gao

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Language: Английский

Citations

0
The efficacy of probiotic mediated hydroxyapatite in mammalian cell culture: promotion of MG–63 and L–929, anti–MCF7 breast cancer cells activities, and cytokine production of PBMC DOI
Sabereh Nouri, Rasoul Roghanian,

Giti Emtiazi

et al.

Emergent Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Language: Английский

Citations

0
Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates DOI
Uğur Azizoğlu, Leandris Argentel‐Martínez, Ofelda Peñuelas‐Rubio

et al.

Journal of Basic Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

ABSTRACT Bacillus cereus group produces diverse antimicrobial compounds through different metabolic pathways, including amino acid‐based compounds, sugar derivatives, volatile and miscellaneous compounds. These exhibit antibacterial antifungal activities against various plant pathogens, promoting growth enhancing tolerance to abiotic stresses. They also nematicidal nematodes antagonistic effects pathogens in aquatic animals, inducing immune responses. Moreover, B. bacteria play a significant role bioremediation by breaking down or neutralizing environmental pollutants, such as plastics, petroleum products, heavy metals, insecticides. produce enzymes like laccases, lipases, proteases, oxidases, contributing the degradation of these pollutants. In food industry, they can cause poisoning due their production enterotoxins. However, are utilized industrial applications, producing environmentally friendly bio‐based materials, biofertilizers, nanoparticles. Notably, transforms selenite into selenium nanoparticles, which have health benefits, cancer prevention. summary, applications agriculture, bioremediation, medicine, sustainable eco‐friendly solutions across multiple fields. this review, we revised characteristics every species; highlighted more important secreted species The aim is explain available secondary metabolites classify from group, increasing knowledge about taxonomy group.

Language: Английский

Citations

0
Exploring the antimicrobial and antioxidative potential of Leucas aspera (Willd.) Link: Phytochemical screening, molecular docking, and HR-LC/MS profiling against SARS-CoV-2 protein 3CLPro, Spike and RDB DOI Creative Commons

Thakur Shalini,

Ragothaman Prathiviraj,

P. Senthilraja

et al.

Phytomedicine Plus, Journal Year: 2024, Volume and Issue: unknown, P. 100700 - 100700

Published: Dec. 1, 2024

Language: Английский

Citations

0
Metagenomic analysis and bioactive profiling of kombucha fermentation: antioxidant, antibacterial activities, and molecular docking insights into gastric cancer therapeutics DOI

Thakur Shalini,

Ragothaman Prathiviraj,

P. Senthilraja

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 13(6)

Published: Nov. 5, 2024

Abstract Kombucha is fermented and produced with a biofilm called symbiotic culture of bacteria yeast, which drunk all over the world for its beneficial effects on human health energy levels. The metagenomic study kombucha frequently detected microorganisms in proteobacteria, firmicutes, actinobacteria. And also, yeast fungi are Ascomycota Basidiomycota present green leaf sugarcane juice kombucha. extracts’ biological activities were assessed using pH, total phenolic content, antioxidant, antibacterial, anticancer activity. Fermentation may enhance activity generation bioactive substances. These results showed pH −3.1 ± 0.2 TPC −0.721 μg/mL gallic acid equivalent. antioxidant radicals scavenging was evaluated by DPPH, ABTS, H2O2 TAC. chemicals identified FT-IR HR-LC/MS analysis totaled 45 components. compounds further move to perform molecular docking against gastric cancer target proteins 4H9M, 2DQ7 1TVO binding Nequinate showing best LibDock scores 105.12, 114.49, 108.97. So, this suggests that knowledge can potentially active it’s stimulated mechanism gastrointestinal transit. Additionally, gives strength understand bacterial fungal distribution from Kombucha.

Language: Английский

Citations

0