Cancers,
Journal Year:
2024,
Volume and Issue:
17(1), P. 27 - 27
Published: Dec. 25, 2024
Background/Objectives:
Despite
the
introduction
of
innovative
therapeutics,
lung
cancer
is
still
leading
cause
cancer-related
death.
For
this
reason,
requires
deep
characterization
to
identify
cellular
and
molecular
targets
that
can
be
used
develop
novel
therapeutic
strategies.
Three-dimensional
models,
including
patient-derived
organoids
(PDOs),
represent
useful
tools
study
biology
may
employed
in
future
as
predictive
decisions.
However,
successful
establishment
cultures
faithfully
respective
patient
tissues
challenging
due
low
success
rate
and/or
overgrowth
normal
airway
epithelial
cells.
Methods:
We
set
up
a
two-step
protocol
allows
for
establishing
both
short-term
long-term
3D
cultures,
with
different
characteristics
rates.
Results:
Cancer
tissue-originated
spheroids
(CTOSs)
show
100%
allow
concomitant
isolation
autologous
tumor
infiltrating
leukocytes
(TILs).
On
contrary,
PDOs
expanded
medium-long
term
bio-banked
but
retain
lower
possibility
contamination
To
overcome
these
problems,
we
an
optimal
medium
formulation
implemented
rigorous
quality
controls,
substantial
improvement
tumoral
PDO
establishment.
Conclusions:
Overall,
guarantees
flexibility
reliability,
also
providing
guidelines
control
checks
support
experimental
settings.
The
setting
robust
culture
expansion
key
requirement
their
employment
research
clinical
practice.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 26, 2024
AbstractBackground:
Renal
cell
carcinoma
(RCC)
is
one
of
the
most
common
diseases
with
limited
treatment
options.
A
better
understanding
this
disease
and
has
been
hindered
by
a
lack
representative
preclinical
models.
Methods:
We
established
tumor
organoids,
three-dimensional
cultures
from
clinical
RCC
samples.
organoids
were
characterized
H&E
staining,
immunohistochemical
staining
whole-exome
sequencing.
Organoids
derived
patients
treated
different
drugs
to
test
their
responses
drugs.
Results:
H&E
sequencing
revealed
that
recapitulated
histological
feature
transcriptional
profile
parent
tumor.
Using
organoid
model,
we
found
exhibited
differential
sunitinib,
pazopanib,
Cabozantinib,
Lenvatinib
+
Everolimus,
MK6482
Sunitinib
treatment.
Conclusions:
Our
research
suggests
may
become
favorable
model
for
precise
drug
use
in
RCC.
Chinese Medical Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 12, 2024
In
multiple
areas
such
as
science,
technology,
and
economic
activities,
it
is
necessary
to
unify
the
management
of
repetitive
tasks
or
concepts
by
standardization
obtain
best
order
high
efficiency.
Organoids,
living
tissue
models,
have
rapidly
developed
in
past
decade.
Organoids
can
be
used
repetitively
for
vitro
culture,
cryopreservation,
recovery
further
utilization.
Because
organoids
recapitulate
parental
tissues'
morphological
phenotypes,
cell
functions,
biological
behaviors,
genomic
profiles,
they
are
known
renewable
"living
biobanks".
cover
two
mainstream
fields:
Adult
stem
cell-derived
(also
patient-derived
organoids)
induced
pluripotent
and/or
embryonic
organoids.
Given
increasing
importance
development
new
drugs,
standardized
operation,
all
steps
organoid
construction
an
important
guarantee
ensure
quality
products.
this
review,
we
systematically
introduce
operation
procedures,
laboratory
construction,
available
documents
related
culture
that
been
published
so
far.
We
also
proposed
challenges
prospects
field.
Cancers,
Journal Year:
2024,
Volume and Issue:
17(1), P. 27 - 27
Published: Dec. 25, 2024
Background/Objectives:
Despite
the
introduction
of
innovative
therapeutics,
lung
cancer
is
still
leading
cause
cancer-related
death.
For
this
reason,
requires
deep
characterization
to
identify
cellular
and
molecular
targets
that
can
be
used
develop
novel
therapeutic
strategies.
Three-dimensional
models,
including
patient-derived
organoids
(PDOs),
represent
useful
tools
study
biology
may
employed
in
future
as
predictive
decisions.
However,
successful
establishment
cultures
faithfully
respective
patient
tissues
challenging
due
low
success
rate
and/or
overgrowth
normal
airway
epithelial
cells.
Methods:
We
set
up
a
two-step
protocol
allows
for
establishing
both
short-term
long-term
3D
cultures,
with
different
characteristics
rates.
Results:
Cancer
tissue-originated
spheroids
(CTOSs)
show
100%
allow
concomitant
isolation
autologous
tumor
infiltrating
leukocytes
(TILs).
On
contrary,
PDOs
expanded
medium-long
term
bio-banked
but
retain
lower
possibility
contamination
To
overcome
these
problems,
we
an
optimal
medium
formulation
implemented
rigorous
quality
controls,
substantial
improvement
tumoral
PDO
establishment.
Conclusions:
Overall,
guarantees
flexibility
reliability,
also
providing
guidelines
control
checks
support
experimental
settings.
The
setting
robust
culture
expansion
key
requirement
their
employment
research
clinical
practice.
