HNF4A-AS1 inhibits the progression of hepatocellular carcinoma by promoting the ubiquitin-modulated degradation of PCBP2 and suppressing the stability of ARG2 mRNA DOI Creative Commons
Wenbo Jia, Yu Liang, Bin Xu

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(13), P. 5087 - 5108

Published: Jan. 1, 2024

Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor with poor prognosis. Extensive research has revealed the significant role of long noncoding RNAs (lncRNAs) in regulation development. In this study, high-throughput sequencing analysis was used to assess expression levels lncRNAs three pairs HCC tissues and their corresponding noncancerous tissues. Through quantitative real-time polymerase chain reaction (qRT-PCR) clinicopathological analysis, it discovered that HNF4A-AS1 downregulated Furthermore, its were found be positively correlated prognosis patients. Subsequent

Language: Английский

The multiple functions and mechanisms of long non-coding RNAs in regulating breast cancer progression DOI Creative Commons
Yongsheng Zhang,

Yanjiao Xu,

Yanping Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 28, 2025

Breast cancer (BC) is a malignant tumor that has the highest morbidity and mortality rates in female population, its high tendency to metastasize main cause of poor clinical prognosis. Long non-coding RNAs (lncRNAs) have been extensively documented exhibit aberrant expression various cancers influence progression via multiple molecular pathways. These lncRNAs not only modulate numerous aspects gene cells, such as transcription, translation, post-translational modifications, but also play crucial role reprogramming energy metabolism by regulating metabolic regulators, which particularly significant advanced BC. This review examines characteristics mechanisms BC both intracellularly (e.g., cell cycle, autophagy) extracellularly microenvironment). Furthermore, we explore potential specific their regulatory factors markers therapeutic targets. Lastly, summarize application treatment BC, aiming offer novel personalized options for patients.

Language: Английский

Citations

0
Circ_0098181 binds PKM2 to attenuate liver fibrosis DOI Creative Commons
Yuanyuan Luo,

Yaping Guan,

Hongsheng Zhan

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

Liver cirrhosis seriously harms human health and fibrosis is the essential pathological process of cirrhosis. Recently, circular RNAs (circRNAs) were found to play critical roles in liver fibrosis, but key circRNAs precise mechanisms remained unclear. This study aimed investigate effect circ_0098181 fibrogenesis explore its mechanism. RNA sequencing was conducted identify circRNA signatures cirrhotic tissues. Hepatic stellate cells (HSCs) (including primary rat HSCs, LX2, HSC-T6) carbon tetrachloride (CCl4) induced model used role on HSC activation vitro vivo. sequencing, pull-down, mass spectrometry, immunoprecipitation (RIP) experiments performed elucidate Circ_0098181 obviously reduced fibrotic tissues activated HSCs. Exogenous administration blocked activation, proliferation, migration HSCs mitigated progression CCl4-induced Mechanistically, adenosine deaminase acting RNA1 (ADAR1) combined with intronic complementary sequences (ICSs) flanking regions, thereby regulating biogenesis circ_0098181. qRT-PCR revealed suppression pro-inflammation cytokines expression (TNFα, Fas, Cxcl11, etc.). RIP indicated that pyruvate kinase M2 (PKM2) direct target bound PKM2, restrained nuclear translocation phosphorylation. In conclusion, exerts a significant anti-fibrotic by binding PKM2 repress inhibiting hepatic inflammation, suggesting promising therapeutic merit

Language: Английский

Citations

0
tRF‐34‐P4R8YP9LON4VHM Promotes Hepatocellular Carcinoma Progression and Tumour Cell‐Induced Angiogenesis via the MEK/ERK Pathway DOI Creative Commons

Tianxin Xu,

Hua Han, Fei Song

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(8)

Published: April 1, 2025

ABSTRACT Hepatocellular carcinoma (HCC) is the most common form of primary liver cancer and poses a significant global health challenge. In recent years, tRNA‐derived small RNAs (tsRNAs) have gained attention due to their potential role in various cancers, including HCC. this study, we reported that tRF‐34‐P4R8YP9LON4VHM expression was elevated HCC tissues cell lines. The association between patients' clinicopathological parameters determined using tissue microarrays 90 patients, found it positively associated with level AFP, tumour size, microvascular density (MVD), TNM stage. We performed CCK8, colony formation assay, EdU, cycle analysis, transwell tube assay verify could enhance proliferation, migration, invasion, cell‐induced angiogenesis vitro vivo. Mechanistically, downregulate DAB2IP by directly targeting its 3'‐UTR, consequently activating MEK/ERK signalling pathway promoting secretion VEGFA from cells into supernatant. conclusion, our research indicated might act as crucial player molecular mechanisms provide novel treatment strategies for patients.

Language: Английский

Citations

0
Exploring the microRNA-mitochondrial nexus in hepatocellular carcinoma DOI
Ali Jawad Akki, Srinivas Nanduri,

Shankargouda Patil

et al.

Mitochondrion, Journal Year: 2025, Volume and Issue: 84, P. 102045 - 102045

Published: April 24, 2025

Language: Английский

Citations

0
WDR11-DT enhances radiosensitivity via promoting PARP1 degradation and homologous recombination deficiency DOI
Yanting Yang, Zongcheng Li, Xinyuan Yu

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217757 - 217757

Published: May 1, 2025

Language: Английский

Citations

0
Glycolysis-associated lncRNAs in cancer energy metabolism and immune microenvironment: a magic key DOI Creative Commons
Xi Zhang, Yunchao Zhang, Qiong Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 13, 2024

The dependence of tumor cells on glycolysis provides essential energy and raw materials for their survival growth. Recent research findings have indicated that long chain non-coding RNAs (LncRNAs) a key regulatory function in the glycolytic pathway offer new opportunities cancer therapy. LncRNAs are analogous to during glycolysis. In this paper, we review mechanisms LncRNA potential therapeutic strategies, including current alterations cancer-related metabolism with lncRNA mediating expression enzymes, lactate production transport, mechanism interaction transcription factors, miRNAs, other molecules. Studies targeting LncRNA-regulated pathways also possibility developing strategies. By regulating expression, metabolic can be interfered inhibit growth metastasis, thus affecting immune drug resistance cells. addition, lncRNAs capacity as molecular markers target therapies, thereby contributing novel strategies approaches field personalized therapy prognosis evaluation. conclusion, LncRNA, molecules pathway, reveals abnormal Future will more thoroughly investigate specific regulation develop corresponding fostering optimism realization precision medicine.

Language: Английский

Citations

2
Identification and Verification of a Glycolysis-Related lncRNA Prognostic Signature for Hepatocellular Carcinoma DOI

Fakai Qiu,

Guozheng Yu,

Mei Li

et al.

Hormone and Metabolic Research, Journal Year: 2024, Volume and Issue: 56(11), P. 827 - 834

Published: May 21, 2024

Abstract Hepatocellular carcinoma (HCC) is a primary liver cancer with high mortality rate. The search for new biomarker could help the prognosis of HCC patients. We identified glycolytic gene set associated and lncRNA based on TCGA MsigDB databases. According to these lncRNAs, K-means clustering, regression analysis were performed Two groups patients different expression levels obtained clustering results. results difference enrichment showed that DEmRNA in two populations significant survival differences was mainly enriched transmembrane transporter complex, RNA polymerase II specificity, cAMP signaling pathway, calcium pathway. In addition, prognostic model 4 DElncRNAs constructed analysis. ROC curve had good predictive performance. Drug predictionresults efficacy JQ1, niraparib, teniposide higher low-risk group than high-risk group. conclusion, this study preliminarily glycolytic-related features lncRNAs risk assessment model. are expected guide clinical

Language: Английский

Citations

0
LINC01370 suppresses hepatocellular carcinoma proliferation and metastasis by regulating the PI3K/AKT pathway DOI Creative Commons
Fei Xiao,

Zhuoyun Zhang,

Luqian Li

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 1, 2024

Abstract Background Hepatocellular carcinoma (HCC) poses a serious threat to human health worldwide. lncRNA dysregulation is frequently observed in various cancers, including HCC. However, the function of LINC01370 HCC progression and its underlying mechanisms remain unclear. Methods expression tissues with cells was analyzed by applying GEO GEPIA databases qRT-PCR. CCK-8 Transwell assays were used assess cell proliferation, migration, invasion. The PI3K, AKT, p-AKT protein western blotting. Results Gene Expression Omnibus (GEO) Profiling Interactive Analysis (GEPIA) showed that significantly lower than normal tissues. overexpression markedly repressed HepG2 SMMC-7721 To understand downstream mechanism regulation, we further genes co-expressed GSE136247 GSE132037 then performed KEGG analysis. PA pathway found be regulated via gene co-expression Furthermore, PI3K levels decreased after overexpression. Importantly, rescue experiments activation PI3K/AKT disrupted repressive effect on invasion cells. Conclusions This study verified suppresses proliferation metastasis regulating pathway.

Language: Английский

Citations

0
The Role of Pyruvate Kinase M2 Posttranslational Modification in the Occurrence and Development of Hepatocellular Carcinoma DOI
Zhao Chunlian, Qi Wan, Rui Zhao

et al.

Cell Biochemistry and Function, Journal Year: 2024, Volume and Issue: 42(7)

Published: Sept. 26, 2024

Hepatocellular carcinoma (HCC) is one of the deadly malignant tumors that directly leads to death nearly million people worldwide every year, causing a serious burden on society. In presence sufficient oxygen, HCC cells rapidly generate energy through aerobic glycolysis, which promotes tumor cell proliferation, immune evasion, metastasis, angiogenesis, and drug resistance. Pyruvate kinase M2 (PKM2) key rate-limiting enzyme in glycolysis. recent years, studies have found PKM2 not only exerts pyruvate activity process glucose metabolism, but also protein non-metabolic pathways affect processes, its flexibly regulated by various posttranslational modifications such as acetylation, phosphorylation, lactylation, ubiquitination, SUMOylation, so forth. This review summarizes role PKM2-related sites development HCC.

Language: Английский

Citations

0
HNF4A-AS1 inhibits the progression of hepatocellular carcinoma by promoting the ubiquitin-modulated degradation of PCBP2 and suppressing the stability of ARG2 mRNA DOI Creative Commons
Wenbo Jia, Yu Liang, Bin Xu

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(13), P. 5087 - 5108

Published: Jan. 1, 2024

Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor with poor prognosis. Extensive research has revealed the significant role of long noncoding RNAs (lncRNAs) in regulation development. In this study, high-throughput sequencing analysis was used to assess expression levels lncRNAs three pairs HCC tissues and their corresponding noncancerous tissues. Through quantitative real-time polymerase chain reaction (qRT-PCR) clinicopathological analysis, it discovered that HNF4A-AS1 downregulated Furthermore, its were found be positively correlated prognosis patients. Subsequent

Language: Английский

Citations

0