Angiotensin converting enzyme expression on circulating immune cells from colorectal cancer patients correlates with the disease stage. Results from a preliminary study.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 26, 2024

Colorectal cancer (CRC) is the third most common and second leading cause of cancer-related mortality worldwide. Because CRC a very complex disease, sometimes patients at same disease stage are treated with combinations systemic therapy surgery to different outcomes. Immune T B cells infiltrating tumor has been associated disease-free survival, cancer-specific survival overall survival. In opposite, Myeloid-derived suppressor linked poor outcome. The peripheral blood evaluation immune less invasive, easy be performed in follow-up visits, could used as guide for interventions. expression angiotensin converting enzyme (ACE) can biomarker prognosis an also target treatment since ACE macrophages neutrophils related improved immunity. Our aim therefore was investigate subsets T, B, myeloid-derived from patients. We found that increased Naïve Effector III. These findings open opportunities targeting new colorectal cancer.

Language: Английский

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NF-κB-activated oncogene inhibition strategy for cancer gene therapy

Cancer Gene Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

NF-κB is a promising target for cancer treatment because of its overactivation in almost all cancers but countless inhibitors rarely became clinical drugs due to side effects. In contrast traditional treatments aimed at inhibiting activity, this study develop novel approach termed HOPE, which focuses on activating the exogenous effector gene CRISPR-Cas13a within cells, achieved by utilizing NF-κB-specific promoter DMP previously constructed, then targets and suppresses expression oncogenes TERT, PLK1, KRAS MYC mRNA level. We evaluated antitumour effects HOPE various cultured cells confirmed it could induce obvious death without affecting normal cells. By packaging into adeno-associated virus (AAV) intravenously injected treat mice that were subcutaneously transplanted with colorectal cancer. This validated rAAV-HOPE significantly inhibit tumour growth Based scRNA-seq data, we observed activate immune system decrease proportion particularly reducing stemness elucidates an important role cell both vitro vivo, additionally provides therapeutic technology therapy.

Language: Английский

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SIngle cell level Genotyping Using scRna Data (SIGURD)

Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 25(6)

Published: Sept. 23, 2024

Abstract Motivation: By accounting for variants within measured transcripts, it is possible to evaluate the status of somatic using single-cell RNA-sequencing (scRNA-seq) and characterize their clonality. However, sparsity (very few reads per transcript) or bias in protocols (favoring 3’ ends transcripts) makes chance capturing very unlikely. This can be overcome by targeted sequencing use mitochondrial as natural barcodes clone identification. Currently, available computational tools focus on genotyping, but do not provide functionality combined analysis functional such characterization gene expression changes detected clones. Results: Here, we propose SIGURD (SIngle cell level Genotyping Using scRna Data) (SIGURD), which an R-based pipeline clonal scRNA-seq data. allows quantification clones leveraging both variants. also after detection: association with populations, detection differentially expressed genes across clones, demonstrate power analyzing data colony-forming cells derived from patients myeloproliferative neoplasms.

Language: Английский

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Research Progress of the Effects of Anesthetic Drugs on the Immune System of Patients with Colorectal Cancer

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(12), P. 66 - 74

Published: Jan. 1, 2024

Language: Английский

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Intratumoural CD8+ CXCR5+ follicular cytotoxic T cells have prognostic value and are associated with CD19+ CD38+ B cells and tertiary lymphoid structures in colorectal cancer

Cancer Immunology Immunotherapy, Journal Year: 2024, Volume and Issue: 74(1)

Published: Dec. 30, 2024

Colorectal cancer (CRC) is the most common digestive in world. Microsatellite stability (MSS) and microsatellite instability (MSI-high) are important molecular subtypes of CRC closely related to tumor occurrence progression immunotherapy efficacy. The presence CD8

Language: Английский

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