Evaluating TcAs for Use in Biotechnology Applications

BioTech, Journal Year: 2025, Volume and Issue: 14(1), P. 5 - 5

Published: Jan. 25, 2025

ABC toxin complexes (Tcs) are tripartite that come together to form nano-syringe-like translocation systems. Tcs often compared with Bacillus thuringiensis (Bt) toxins, and as such, they have been highly studied a potential novel pesticide combat growing insect resistance. Moreover, it is possible substitute the cytotoxic hypervariable region alternative peptides, which promise use peptide delivery system. These toxins possess unique ability active chimeric holotoxins across species display capability translocate variety of payloads membrane bilayers. Additionally, mutagenesis on linker receptor binding domains (RBDs) show mutations do not inherently cause loss functionality for translocation. For these reasons, emerged an ideal candidate targeted protein engineering. However, elucidation specific function each RBD in relation target recognition currently limits rational design approach any Tc. there distinct lack targeting biodistribution data many among mammals mammalian cell lines. Here, we outline two separate strategies modifying capabilities A subunit (TcA) from Xenorhabdus nematophilus, Xn-XptA2. We identify structural differences make Xn-XptA2 different than other characterized TcAs modular substituting RBDs into scaffold. Finally, first, our knowledge, TcA mice.

Language: Английский

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Research progress on cholesterol metabolism and tumor therapy

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 30, 2025

Cholesterol and its metabolic derivatives have important biological functions are crucial in tumor initiation, progression, treatment. maintains the physical properties of cellular membranes is pivotal cell signal transduction. metabolism includes both de novo synthesis uptake from extracellular sources such as low-density lipoprotein (LDL) high-density (HDL). This review explores aspects to provide a comprehensive understanding their roles cancer. involved bile acid production steroid hormone biosynthesis closely linked reprogramming endogenous exogenous signals within microenvironment. These intricately associated with key processes proliferation, survival, invasion, metastasis. Evidence suggests that regulating cholesterol may offer therapeutic benefits by inhibiting growth, remodeling immune microenvironment, enhancing antitumor responses. summarizes role biology discusses application statins other inhibitors cancer therapy, aiming novel insights for development drugs targeting advances diagnosis

Language: Английский

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Mobocertinib antagonizes multidrug resistance in ABCB1- and ABCG2-overexpressing cancer cells: in vitro and in vivo studies

Cancer Letters, Journal Year: 2024, Volume and Issue: unknown, P. 217309 - 217309

Published: Oct. 1, 2024

Language: Английский

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Ketogenesis promotes triple-negative breast cancer metastasis via calpastatin β-hydroxybutyrylation

Lipids in Health and Disease, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 12, 2024

Triple-negative breast cancer (TNBC) continues to pose a significant obstacle in the field of oncology. Dysregulation lipid metabolism, notably upregulated ketogenesis, has emerged as hallmark TNBC, yet its role metastasis been elusive. Here, by utilizing clinical specimens and experimental models, study demonstrates that increased ketogenesis fosters TNBC promoting up-regulation β-hydroxybutyrate (β-OHB), key ketone body. Mechanistically, β-OHB facilitates β-hydroxybutyrylation (Kbhb) Calpastatin (CAST), an endogenous calpain (CAPN) inhibitor, at K43, blocking interaction with CAPN subsequently FAK phosphorylation epithelial‒mesenchymal transition (EMT). In conclusion, reveals novel regulatory axis linking metastasis, shedding light on intricate interplay between metabolic reprogramming tumor progression.

Language: Английский

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DNA Repair Status as a Guide for Pancreatic Ductal Adenocarcinoma Treatment, an Old View for a New Future.

Cancer Letters, Journal Year: 2024, Volume and Issue: 604, P. 217222 - 217222

Published: Sept. 7, 2024

Language: Английский

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The BET inhibitor sensitivity is associated with the expression level of CDC25B in pancreatic cancer models

Cancer Drug Resistance, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Cell division cycle 25B (CDC25B) belongs to the CDC25 family of phosphatases that regulate cell progression. CDC25B also contributes tumor initiation and progression, but no connection between levels drug sensitivity in pancreatic cancer has been reported. Based on our finding bromodomain extraterminal domain (BET) inhibitors decrease CDC25B, we aim compare models expressing contrasting BET inhibitor JQ1, lines

Language: Английский

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