Protein Purification Using Chitosan‐Modified Polyacrylonitrile Membranes DOI

Gizele G. F. Andrade,

Andressa Nathally Rocha Leal, Jeferson Gomes de Andrade

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

ABSTRACT Protein purification is essential for biomedical and industrial applications, but current methods often face challenges like low efficiency, high cost, limited selectivity. To overcome these drawbacks, we developed electrospun polyacrylonitrile (PAN) membranes with chitosan (Cs) evaluated their ability to capture bovine serum albumin (BSA) immunoglobulin G (IgG) from aqueous solutions. Comparing PAN/Cs pristine PAN membranes, showed Cs significantly enhances BSA adsorption. The were characterized using scanning electron microscopy, Fourier Transform Infrared Spectroscopy, tensile testing, surface area analysis, energy‐dispersive X‐ray spectroscopy, contact angle measurements. exhibited smooth, uniform fibers averaging 0.454 μm in diameter. maximum adsorption capacity () was 75.61 mg g −1 21.17 PAN, while IgG, 28.83 19.77 PAN. SDS‐PAGE electrophoresis confirmed that the eluted proteins maintained quality integrity. Elution efficiency of reached 89.13% 62.16% IgG. Additionally, selectively captured IgG when co‐dissolved These results position as promising materials efficient, cost‐effective, selective protein purification, potential applications both fields.

Language: Английский

Polysaccharide-based biopolymeric magnetic hydrogels for remediation of antibiotics from aqueous solution DOI Creative Commons
Gaurav Sharma, Akshay Verma, Alberto García‐Peñas

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137555 - 137555

Published: Nov. 12, 2024

Polysaccharide-based biopolymeric magnetic hydrogels have garnered significant attention as effective materials for wastewater treatment due to their high adsorption capacity and environmentally friendly nature. This review examines recent advancements in the development of derived from polysaccharides such cellulose, chitosan, alginate, carrageenan, starch, gums, with a focus on application removing antibiotics contaminated water it not only enhances performance but also simplifies separation processes after treatment, making them highly efficient practical applications. The aims provide comprehensive overview synthesis techniques, characteristics, interaction mechanisms these hydrogels, highlighting renewability suitability large-scale treatment. Despite promise, there is lack in-depth analysis fabrication methods. addresses this gap by evaluating various methods assessing hydrogels' efficiency adsorbing antibiotic pollutants. Key findings reveal that components contribute materials' enhanced binding, better removal capabilities, easy recoverability. between are explored, demonstrating superior potential. Future challenges research directions discussed, an emphasis improving scalability applications hydrogels. Overall, offers valuable insights into potential towards purification.

Language: Английский

Citations

3
Advancing wastewater treatment: chitin and derivatives for PPCP contaminant mitigation DOI Creative Commons

P. V. Atheena,

Renuka Basawa,

Ritu Raval

et al.

Polymer Bulletin, Journal Year: 2024, Volume and Issue: 81(16), P. 14307 - 14336

Published: July 18, 2024

Abstract There is a growing threat of wastewater pollution impacting human access to clean water. The surge in pharmaceuticals and personal care products (PPCPs) such water has reached alarming levels, posing an unprecedented aquatic ecosystems health. Conventional treatment not specifically tailored address the presence PPCPs. This paper examines possibility using nano-chitin chitosan as effective sustainable adsorbents for removal PPCPs from wastewater. recent progress functionalized or composites reviewed with attention enhanced adsorption capacity selectivity toward different classes Unique features chitosan, their large surface areas biocompatibility making them good choice PPCP adsorption, are also discussed. Critical evaluation given about how these nanomaterials absorb by looking at various mechanisms like electrostatic interactions, hydrogen bonding, hydrophobic interaction, among others. Finally, future research directions provided improving performance scalability bio-based Graphical

Language: Английский

Citations

2
Hybrid composite beads of chitosan/graphene oxide for dye adsorption: characterizations, kinetic modeling and toxicity study DOI

Nikoleta Syngelaki,

Sofia L. Kouvalakidou,

Konstantinos N. Maroulas

et al.

Journal of Dispersion Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Oct. 23, 2024

The current study aimed to the preparation of graphene oxide impregnated chitosan– polyvinyl alcohol hybrid beads (GO/CS-PVA) for removal Reactive Black 5 (RB5) dye from wastewater solutions, presenting them as a cost-effective alternative treatment. These were fabricated using dripping technique in an aqueous solution. surface and structure material before after adsorption process studied by analyzing FTIR spectroscopy Scanning electron microscope (SEM) well capacity was also evaluated. results proved successful synthesis GO/CS-PVA elucidated physical mechanism RB5. SEM images revealed uniform spherical with rough surface, approximately 752 μm diameter. Additionally, evaluation showed that optimum condition RB5 is at pH = 3 while kinetic investigations adhered pseudo-second-order model, indicating chemisorption reaction. Furthermore, increase temperature 25 65 °C enhanced maximum 34%. cytotoxic effect measured on adipose derived stem cells via MTT assay negligible. In conclusion, it eco-friendly adsorbents can efficiently remove practical applications.

Language: Английский

Citations

2
Environmental toxicity and biodegradation of chitosan derivatives: A comprehensive review DOI
Ioanna Koumentakou, Anastasia Meretoudi, Christina Emmanouil

et al.

Journal of Industrial and Engineering Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

1
A biosensor based on MWCNTs-BSA and TiO2-Laccase nanocomposite modified glassy carbon electrode for sensitive detection of luteolin in traditional Chinese Medicine DOI
Kexin Xu, Linlin Ma, Yujie Chen

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112103 - 112103

Published: Nov. 1, 2024

Language: Английский

Citations

0
Protein Purification Using Chitosan‐Modified Polyacrylonitrile Membranes DOI

Gizele G. F. Andrade,

Andressa Nathally Rocha Leal, Jeferson Gomes de Andrade

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

ABSTRACT Protein purification is essential for biomedical and industrial applications, but current methods often face challenges like low efficiency, high cost, limited selectivity. To overcome these drawbacks, we developed electrospun polyacrylonitrile (PAN) membranes with chitosan (Cs) evaluated their ability to capture bovine serum albumin (BSA) immunoglobulin G (IgG) from aqueous solutions. Comparing PAN/Cs pristine PAN membranes, showed Cs significantly enhances BSA adsorption. The were characterized using scanning electron microscopy, Fourier Transform Infrared Spectroscopy, tensile testing, surface area analysis, energy‐dispersive X‐ray spectroscopy, contact angle measurements. exhibited smooth, uniform fibers averaging 0.454 μm in diameter. maximum adsorption capacity () was 75.61 mg g −1 21.17 PAN, while IgG, 28.83 19.77 PAN. SDS‐PAGE electrophoresis confirmed that the eluted proteins maintained quality integrity. Elution efficiency of reached 89.13% 62.16% IgG. Additionally, selectively captured IgG when co‐dissolved These results position as promising materials efficient, cost‐effective, selective protein purification, potential applications both fields.

Language: Английский

Citations

0