The Journal of V N Karazin Kharkiv National University Series Biology, Journal Year: 2024, Volume and Issue: 43, P. 96 - 104
Published: Dec. 23, 2024
The objective was to ascertain the potential of cryo-umbilical cord extract as an alternative fetal bovine serum (FBS), which is conventionally employed stimulate cell growth but subject ethical and safety constraints. umbilical cryo-extract a rich source bioactive components that have been demonstrated enhance adhesive, proliferative metabolic activity cells. Furthermore, it has shown be effective substitute for in cultivation various types, making promising candidate use cellular technology regenerative medicine. findings experimental study cryoextract exhibited notable enhancement adhesive attributes cells, when compared other conditions were investigated. fibroblasts high adhesion rapid migration presence cryoextract, provides advantages restoration connective tissue stroma organs skin. nerve cells enhanced viability stability comparison FBS, renders incorporation into neuroregenerative technologies. substantial beneficial impact on liver augmenting their adhesion, thereby opening avenues investigation regeneration. effect splenocytes also favorable, indicating can bolster immune function circumstances where response regulation necessary. lyophilized limited positive effect, particularly relation its properties. However, this less pronounced than observed cryoextract. addition resulted reduction activity, necessity optimization lyophilization process preserve biological extract. capacity long-term storage render valuable resource situations preservation prolonged shelf life are significance. Thus, represents provide necessary support growth, proliferation. Nevertheless, production lyophilizate must undergo further refinement order attain same efficacy Further research should concentrate comprehensive examination mechanisms action techniques adapt product requirements This will facilitate wider application stimulation regeneration functional organs.
Language: Английский