Induction of mitochondrial toxicity by non-steroidal anti-inflammatory drugs (NSAIDs): The ultimate trade-off governing the therapeutic merits and demerits of these wonder drugs

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 228, P. 116283 - 116283

Published: May 14, 2024

Language: Английский

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Progress and application of lung-on-a-chip for lung cancer

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: May 24, 2024

Lung cancer is a malignant tumour with the highest incidence and mortality worldwide. Clinically effective therapy strategies are underutilized owing to lack of efficient models for evaluating drug response. One main reasons failure anticancer development resistance. Anticancer drugs face severe challenges such as poor biodistribution, restricted solubility, inadequate absorption, accumulation. In recent years, “organ-on-a-chip” platforms, which can directly regulate microenvironment biomechanics, biochemistry pathophysiology, have been developed rapidly shown great potential in clinical research. Lung-on-a-chip (LOC) new 3D model bionic lungs physiological functions created by micromachining technology on microfluidic chips. This approach may be able partially replace animal 2D cell culture models. To overcome resistance, LOC realizes personalized prediction response simulating lung-related vitro , significantly enhancing therapeutic effectiveness, bioavailability, pharmacokinetics while minimizing side effects. this review, we present an overview advances preparation contrast it earlier Finally, describe LOC. The combination nanomedicine will provide accurate reliable treatment preclinical evaluation.

Language: Английский

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Amphotericin B Encapsulation in Polymeric Nanoparticles: Toxicity Insights via Cells and Zebrafish Embryo Testing

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 116 - 116

Published: Jan. 16, 2025

Background: Amphotericin B (AmB) is a commonly utilized antifungal agent, which also recommended for the treatment of certain neglected tropical diseases, including leishmaniasis. However, its clinical application constrained because poor oral bioavailability and adverse effects, prompting investigation alternative drug delivery systems. Polymeric nanoparticles (PNPs) have gained attention as potential vehicle, providing advantages such sustained release enhanced bioavailability, could AmB carriers. concerns persist regarding nanomaterials' toxicity, requiring more studies. Zebrafish (Danio rerio) embryos were used valuable model toxicity testing, especially their genetic similarity to humans standardized developmental assessments. Methods: In this study, we produced characterized loaded non-loaded PNPs by nanoprecipitation, dynamic light scattering, transmission electron microscopy, atomic force microscopy spectroscopy. Afterwards, verified through in vitro MTT assays three cell lines (HEK293, HepG2, J774 A1) vivo tests with zebrafish embryos. Results: both trials, it was noted that nanoencapsulation led increased when compared non-encapsulated AmB, possibly indicating they penetrated embryo's chorion. Nevertheless, demonstrated polymers are safe not cause neither nanostructures per se. Conclusions: Therefore, believed objective improving may been achieved, observed probably linked AmB's ability destabilize membranes.

Language: Английский

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Optimizing chronic kidney disease management: The potential of a multi-strain probiotic formulation

World Journal of Nephrology, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 18, 2025

Chronic kidney disease (CKD), which represents a significant global health concern, is characterized by gradual decline in function, leading to complications such as electrolyte imbalance, cardiovascular disease, and immune dysfunction. Standard CKD management includes dietary modifications, ketoanalogues supplementation, blood pressure glucose control, hydration maintenance, treatment of the underlying causes. Emerging evidence has indicated role gut microbiota CKD, that dysbiosis contributes progression towards end-stage renal disease. Probiotics prebiotics have recently garnered attention owing their potential enhance gastrointestinal well-being restoring balance microbiota. Specific probiotic strains, including Lactobacillus Bifidobacterium , promote beneficial bacterial growth, suppress harmful bacteria, exert anti-inflammatory, antihypertensive, antidiabetic effects. The combination Streptococcus thermophilus acidophilus longum Bacillus coagulans demonstrated therapeutic formulation for various studies, highlighting its promise treating CKD; however, supporting remains limited, making it crucial conduct further investigations determine specific effects different formulations on outcomes patients with CKD.

Language: Английский

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Assessment of Potentially Nephrotoxic Drug Prescriptions in Chronic Kidney Disease Outpatients at a Hospital in Indonesia

International Journal of Nephrology and Renovascular Disease, Journal Year: 2025, Volume and Issue: Volume 18, P. 59 - 69

Published: Feb. 1, 2025

Nephrotoxic drugs can worsen the kidney function of patients with chronic disease (CKD). There is still a limited amount research investigating nephrotoxic in Indonesia. This study aims to analyze prevalence potentially (PND) prescriptions and association patients' characteristics PND prescribing. cross-sectional employed retrospective data from Universitas Indonesia Hospital (RSUI), focusing on CKD outpatients treated between January 2019 December 2022. over age 18 were included, exclusions for those suspected having CKD, history transplants, or missing critical data. The outcome was prescribed PND, determined using reliable references assess potential nephrotoxicity. Furthermore, compliance clinical guidelines evaluated at individual drug level, each within prescription as separate case. Descriptive analyses carried out determine prevalence, which presented percentages. Logistic regression analysis performed examine patient PND. In total, 248 evaluated. findings revealed that 177 (71.4%) least one categories these included antihypertensives (50.9%), antigout medications (17.8%), antiplatelets (10.5%), antibiotics (9.8%), NSAIDs (5.8%), antiulcer agents (5.2%). Of 275 cases prescriptions, 220 (80.0%) complied treatment guidelines, while 55 (20.0%) did not. indicated taking more than four additional likely be PNDs fewer (aOR 2.454, 95% CI 1.399-4.305). Although non-compliance are relatively low, frequently patients, risk rising number comedications increases. Measures needed ensure guideline compliance, including accurate dosage assessments monitoring.

Language: Английский

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Effect of N-acetylcysteine on antimicrobials induced nephrotoxicity: a meta-analysis

BMC Nephrology, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 8, 2025

N-acetylcysteine (NAC) has antioxidant effects in reducing acute kidney injury. This study systematically reviewed and assessed the efficacy of NAC preventing antimicrobials induced nephrotoxicity. Pubmed, Embase, Web Science, Cochrane Library were searched extensively for relevant studies that evaluating on nephrotoxicity until June 1, 2024. Eligible records screened according to inclusion exclusion criteria. The odds ratio (OR) was selected evaluate effect We pooled extracted data using a random model. Three randomized controlled trials included analysis. results showed could reduce incidence (OR = 0.487, 95% CI 0.258, 0.918, P 0.03, I2 0%). Serum creatine (Scr) Day 2 significantly decreased group compared placebo (SMD, − 0.298; 95%CI, 0.585 0.010; 23%; 0.04). No difference observed blood urea nitrogen (BUN), creatinine clearance (CrCl). In this meta-analysis, associated with benefit prevention However, large-scaled well-designed RCTs are required future.

Language: Английский

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Effects of Zofenopril and Thymoquinone in Cyclophosphamide-Induced Urotoxicity and Nephrotoxicity in Rats; The Value of Their Anti-Inflammatory and Antioxidant Properties

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 3657 - 3676

Published: March 1, 2025

Objective: The study aimed to investigate whether zofenopril (ZOF), thymoquinone (TQ), or their co-administration effectively ameliorates urotoxicity and nephrotoxicity following cyclophosphamide (CPH) treatment. Methods: A total of 48 Wister Albino female rats were divided into six groups each eight rats; negative control (NC), positive (PC), mesna (MS), ZOF, TQ, ZOF+TQ groups. Normal saline, mesna, ZOF-15mg/kg, TQ-80mg/kg, combination given orally for 19 days the NC, MS, respectively. On 17 th day, a single dose CPH 200 mg/kg was intraperitoneally all except NC group. Urine collected over 24 hours before animal scarification urinalysis. After scarification, blood, kidney tissue obtained assessment conventional function parameters, novel injury biomarkers, pro-inflammatory cytokines, oxidative status, complete blood count (CBC), histopathological examination. Results: disturbed urinary excretion urea, creatinine, protein, significantly elevated biomarkers including cystatin-C (Cys-C) (p=0.019) markedly molecule-1 (KIM-1) (p=0.27), semiquantitative measurement hematuria revealed significant elevation score (p=0.0002), urine pus protein (p=0.0005). Additionally, CBC-derived inflammatory neutrophil-lymphocyte ratio (NLR) (p=0.001), neutrophil-monocyte (NMR) (p=0.0004), cytokine interleukin (IL)-6 (p=0.016) tumor necrosis factor (TNF)-α (p<=0.007), antioxidant capacity (TAC) (p< 0.0001) increased. Evidence obvious structural alteration noticed in bladder urothelium CPH-treated animals. co-treatment prevented these deleterious effects associated with Conclusion: This demonstrated that ZOF TQ provided uroprotective nephroprotective against CPH-induced by reducing markers, restoring capacity, improving outcomes. suggested mechanism involves anti-inflammatory activity sulfhydryl-angiotensin converting enzyme inhibitor ZOF. Keywords: anti-inflammatory, cyclophosphamide, hemorrhagic cystitis, kidney, stress

Language: Английский

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Implementation and One-Year Evaluation of Proenkephalin A in Critical Care

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2602 - 2602

Published: March 13, 2025

Proenkephalin A 119–159 (PENK) is a promising functional kidney biomarker, evaluated in various clinical settings. In critical care medicine, early diagnosis of acute injury (AKI) crucial; however, to date, the and assessment function still based on serum creatinine (sCr) urine output, both associated with several limitations. Between November 2020 March 2022, we implemented PENK our daily practice intensive units (ICU). PENK, sCr, AKI stage, start duration renal replacement therapy (RRT) were documented. Almost 18,000 measurements from 4169 patients analyzed, glomerular filtration rate (GFR) was estimated new PENK-GFR formula. outperformed sCR trajectory over time. Moreover, predicted use RRT thus showed its usefulness practice.

Language: Английский

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Lactobacillus paracasei Activates the KDM3A/VAMP1 Axis to Induce Autophagy in Renal Tubular Epithelial Cells in Chronic Kidney Disease

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Language: Английский

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Pinitol Improves Lipopolysaccharide-Induced Cellular Damage in Human Dermal Microvascular Endothelial Cells

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1513 - 1513

Published: March 28, 2025

3-O-Methyl-D-chiro-inositol (pinitol) has been reported to possess insulin-like effects and is known as one of the anti-diabetic agents for improving muscle liver function. However, beneficial pinitol on human dermal microvascular endothelial cells (HDMECs) are not well understood. In this study, we investigated whether could protect HDMECs from damage induced by lipopolysaccharides (LPSs), which cause various cell defects. We observed that enhanced wound healing LPS-damaged HDMECs. found significantly downregulated LPS-induced upregulation reactive oxygen species (ROS). Pinitol also restored mitochondrial membrane potential in these cells. Immunofluorescence analysis revealed notably reduced nuclear localization NF-κB Furthermore, demonstrated decreased phosphorylation levels MAPK family Interestingly, improved tube formation Taken together, suggest exerts several may be a promising therapeutic agent vascular-related skin diseases.

Language: Английский

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