Chinese Journal of Organic Chemistry, Journal Year: 2024, Volume and Issue: 44(7), P. 2296 - 2296
Published: Jan. 1, 2024
Language: Английский
ACS Central Science, Journal Year: 2024, Volume and Issue: 10(8), P. 1442 - 1459
Published: July 22, 2024
Limited understanding of human proteoforms with complex posttranslational modifications and the underlying mechanisms poses a major obstacle to research on health disease. This Outlook discusses opportunities challenges
Language: Английский
Citations
12
Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: 157, P. 104899 - 104899
Published: Feb. 1, 2025
Language: Английский
Citations
1
Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: 42(10), P. 1114 - 1120
Published: Feb. 5, 2024
Comprehensive Summary Expressed protein ligation (EPL) provides a powerful tool to access large‐size proteins with precise structures. Existing methods for constructing the critical thioester EPL have predominantly relied on recombinant intein fusion expressed in Escherichia coli (E. ). Despite its applications, expression of derived from eukaryotic E. inherently suffers limited solubility, inactivity intein, premature hydrolysis and low yields. To overcome these obstacles, we present herein facile one‐flask synthesis inaccessible α‐thioester via SUMO‐protein‐intein (SPI) sandwich model. The utility SUMO enhances yield prevents simplifies purification process. internal Cys residues can be readily produced is compatible EPL‐desulfurization protocol used prepare complex proteins, which otherwise difficult obtain using traditional methods. Its has been highlighted through human granulocyte colony‐stimulating factor (G‐CSF).
Language: Английский
Citations
4
Angewandte Chemie International Edition, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
MUC7, a highly glycosylated protein in saliva and respiratory tract, plays potential roles facilitating bacterial clearance preventing microbial invasion. The complexity of glycan structures multiplicity glycosylation sites MUC7 make it very difficult to explore accurate biofunctions against pathogens. Here, we report an efficiently convergent chemoenzymatic approach firstly synthesize O-glycosylated glycopeptides with nine bearing various glycoforms via the combined use hydrophobic tag-assisted liquid-phase peptide synthesis enzymatic-catalyzed elongation. Biological evaluations reveal that different synthetic mediate unique activities biofilm formation Pseudomonas aeruginosa, among which sialylated glycopeptide exhibits better inhibitory activity has develop antibacterial drugs.
Language: Английский
Citations
0
Angewandte Chemie, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Abstract MUC7, a highly glycosylated protein in saliva and respiratory tract, plays potential roles facilitating bacterial clearance preventing microbial invasion. The complexity of glycan structures multiplicity glycosylation sites MUC7 make it very difficult to explore accurate biofunctions against pathogens. Here, we report an efficiently convergent chemoenzymatic approach firstly synthesize O ‐glycosylated glycopeptides with nine bearing various glycoforms via the combined use hydrophobic tag‐assisted liquid‐phase peptide synthesis enzymatic‐catalyzed elongation. Biological evaluations reveal that different synthetic mediate unique activities biofilm formation Pseudomonas aeruginosa , among which sialylated glycopeptide exhibits better inhibitory activity has develop antibacterial drugs.
Language: Английский
Citations
0
Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown
Published: May 8, 2025
Glycosylation plays a critical role in modulating protein and peptide properties, yet the impact of recently discovered natural mucin-type O-glycosylation on therapeutic peptides like glucagon remains underexplored due to challenges obtaining homogeneous research samples. Here, we address this challenge by developing streamlined multistep synthesis-one purification protocol, enabling production 21 glycoforms with systematically varied glycosylation patterns. Investigation these revealed remarkable two properties for glucagon: solubility increased over 870-fold, fibrillation was completely inhibited, even under stringent conditions, while biological activity elevating blood glucose levels preserved. Computational analyses indicated that sialylated glycans disrupt intermolecular associations solid state, enhancing solubility, shift secondary structures from β-sheets α-helices, mitigating fibrillation. These findings suggest may have evolved as mechanism optimize undesirable offering valuable insights development therapeutics based other proteins similar challenges.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144141 - 144141
Published: May 1, 2025
Language: Английский
Citations
0
Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 78, P. 102420 - 102420
Published: Jan. 3, 2024
Language: Английский
Citations
2
Chemical Science, Journal Year: 2024, Volume and Issue: 15(35), P. 14506 - 14512
Published: Jan. 1, 2024
In this work, we developed a novel strategy, prior disulfide bond-mediated Ser/Thr ligation (PD-STL), for the chemical synthesis of peptides and proteins.
Language: Английский
Citations
2
Chinese Journal of Organic Chemistry, Journal Year: 2024, Volume and Issue: 44(7), P. 2296 - 2296
Published: Jan. 1, 2024
Language: Английский
Citations
1