Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 329, P. 125570 - 125570
Published: Dec. 10, 2024
Language: Английский
Inorganic Chemistry Communications, Journal Year: 2025, Volume and Issue: unknown, P. 113986 - 113986
Published: Jan. 1, 2025
Language: Английский
Citations
0
Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 4, 2025
Language: Английский
Citations
0
Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 174 - 174
Published: March 7, 2025
Hydrogen sulfide (H2S) is an important signaling molecule involved in various physiological and pathological processes, making its accurate detection biological systems highly desirable. In this study, two fluorescent probes (M1 M2) based on 1,8-naphthalimide were developed for H2S via a nucleophilic aromatic substitution. M1 demonstrated high sensitivity selectivity aqueous media, with limit of 0.64 µM strong linear fluorescence response the range 0–22 NaHS. The reaction kinetics revealed rapid response, rate constant 7.56 × 102 M−1 s−1, was most effective pH 6–10. Mechanism studies using 1H NMR titration confirmed formation 4-hydroxyphenyl-1,8-naphthalimide as product H2S-triggered applied MDA-MB-231 cells cell imaging, which provided significant enhancement upon NaHS treatment, confirming applicability detecting environments. comparison, M2, designed extended conjugation red-shifted emission, exhibited weaker due to reduced stability naphtholate lower solubility. These results demonstrate that selective probe H2S, providing valuable resource investigating roles health disease.
Language: Английский
Citations
0
Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 2067 - 2079
Published: March 1, 2025
Neurodegenerative diseases are often linked to oxidative stress (OS), which worsen neuroinflammation and cause neuronal damage. Managing OS with gasotransmitters such as hydrogen sulphide (H2S) is a promising therapeutic approach protecting brain cells from AP39, mitochondria-targeted H2S donor, has shown neuroprotective potential by reducing improving mitochondrial function. However, its clinical application limited due poor stability rapid release, necessitating drug delivery system enhance efficacy. This study aimed develop novel AP39-loaded liposomal formulation provide controlled facilitate AP39 permeation across the blood-brain barrier (BBB), assess functional effects in mitigating preserving unilamellar liposomes were prepared via ethanol injection characterised for size, polydispersity, zeta potential. Entrapment efficiency was determined using HPLC, while cytotoxicity assessed human vein endothelial (HUVEC) neuroblastoma (SHSY5Y) cells. Liposomal permeability, release kinetics, cellular uptake evaluated microvasculature BBB model. Mitochondrial function under Seahorse XFe24 Analyzer. had an average size of 135.92 ± 10.05 nm, 17.35 3.40 mV, entrapment 84.48% 4.7. Cytotoxicity studies showed no adverse after 4 h. Cellular encapsulated significantly higher (7.13 0.28 µg) than free form (5.8 0.31 µg). The model demonstrated sustained (7.28 µg/mL vs 6.44 AP39). assays confirmed preserved antioxidant properties enhanced oxygen consumption. Our encapsulating improves stability, promotes enhances permeability effects. These findings indicate that suitable further investigate treatment neurodegenerative diseases.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3131 - 3131
Published: March 28, 2025
Hydrogen sulfide (H2S) has emerged as a pivotal gaseous transmitter in the central nervous system, influencing synaptic plasticity, learning, and memory by modulating various molecular pathways. This review examines recent evidence regarding how H2S regulates NMDA receptor function neurotransmitter release neuronal circuits. By synthesizing findings from animal cellular models, we investigate impacts of enzymatic production exogenous on excitatory currents, long-term potentiation, intracellular calcium signaling. Data suggest that interacts directly with subunits, altering excitability. Simultaneously, promotes neurotransmitters such glutamate GABA, shaping dynamics plasticity. Furthermore, reports indicate disruptions metabolism contribute to cognitive impairments neurodegenerative disorders, underscoring potential therapeutic value targeting H2S-mediated Although precise mechanisms H2S-induced changes strength remain elusive, growing body positions significant regulator formation processes. calls for more rigorous exploration into underpinnings paving way novel pharmacological interventions dysfunction.
Language: Английский
Citations
0
Neurotherapeutics, Journal Year: 2025, Volume and Issue: unknown, P. e00586 - e00586
Published: April 1, 2025
Language: Английский
Citations
0
Bratislavské lekárske listy/Bratislava medical journal, Journal Year: 2025, Volume and Issue: unknown
Published: April 7, 2025
Abstract Objectives This study investigated the effects of H 2 S donor sodium hydrosulfide (NaHS) on demyelination and behavioral deficits in a cuprizone (CPZ)-induced experimental rat model MS. Methods The rats were fed chow pellets supplemented with 1% CPZ for 5 weeks, NaHS (50 100 μmol/kg) was administered during last weeks. For assessment, grip strength, passive avoidance, rota rod elevated plus maze tests performed. Nuclear factor kappa-B (NF-κB) interleukin-1 beta (IL-1β) measured to assess inflammation brain tissue. Demyelination corpus callosum (CC) assessed by immunohistochemistry myelin basic protein (MBP), platelet-derived growth receptor α (PDGFRα) Glial fibrillary acidic (GFAP). TUNEL method used apoptosis. Results CC reduced NaHS-treated groups. increased amount MBP decreased PDGFRα GFAP immunoreactive cells. NF-κB, IL-1β levels, number apoptotic cells In addition, improved muscle motor coordination, learning memory, showed significant anxiolytic effect. However, it found that beneficial at high dose μmol/kg. Conclusion neuroprotective reducing demyelination, apoptosis CPZ-induced MS model. also memory activity. Anti-inflammatory, anti-apoptotic antioxidant may play role S.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19822 - 19832
Published: Nov. 22, 2024
Hydrogen sulfide (H2S) is an important bioactive molecule that plays a significant role in various functions, particularly the living brain, where it closely linked to cognition, memory, and several neurological diseases. Consequently, developing effective detection methods for H2S essential studying brain functions underlying mechanisms of these This study aims construct novel photoelectrochemical (PEC) microelectrode Ti/TiO2@HSP quantitative monitoring levels brain. The PEC formed by covalently bonding specifically designed organic probe HSP, which possesses D-π–A structure, surface TiO2 nanotubes generated via situ anodic oxidation titanium wire. HSP can effectively react with generate photocurrent response under long-wavelength excitation light (560 nm), thereby achieving H2S. sensor demonstrates high sensitivity good selectivity. In vivo experiments utilizing enable dynamic changes across regions mouse findings reveal normal mice, concentration hippocampus significantly higher than striatum cerebral cortex. Additionally, following propargylglycine drug stimulation, concentrations different were observed decrease, most substantial reduction noted hippocampus. suggests cystathionine γ-lyase (CSE) primary enzyme responsible production this area, while exhibits less pronounced decrease concentration, indicating reliance on alternative enzymatic pathways production. Therefore, not only successfully develops high-performance but also provides new experimental tools theoretical foundations further exploring roles neurophysiological pathological processes.
Language: Английский
Citations
3
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 329, P. 125570 - 125570
Published: Dec. 10, 2024
Language: Английский
Citations
2