Exploring the Anti‐Adherence Potential of Skt35 to Combat Catheter‐Associated Staphylococcus aureus Infections: Efficacy, Toxicity and Mechanism of Action

Chemistry & Biodiversity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

ABSTRACT Catheter‐associated urinary tract infections (CAUTIs), often caused by biofilm‐forming Staphylococcus aureus , present significant clinical challenges. Skt35, a dioxopiperidinamide derivative of cinnamic acid, was investigated for its potential antibacterial and antibiofilm activities against S. biofilms. The effect Skt35 assessed using the zone inhibition microdilution methods, revealing minimum inhibitory concentration (MIC) 250 µM. Antibiofilm properties were confirmed through crystal violet assays, scanning electron microscopy confocal laser microscopy, showing biofilm at Sub‐MIC. In an in vitro bladder model, Skt35‐coated silicone catheter tubes exhibited antiadhesive effects. Zebrafish embryo tests indicated no toxicity concentrations up to 125 Molecular docking simulation analysis revealed strong binding affinities Accessory Gene Regulator A (‐7.9 kcal/mol) Lux Small protein (‐4.96 kcal/mol), suggesting disruption quorum sensing gene expression making it promising candidate coatings prevent CAUTIs.

Language: Английский

Exposure to bisphenol A and sodium nitrate found in processed meat induces endocrine disruption and dyslipidemia through PI3K/AKT/SREBP pathway in zebrafish larvae

The Journal of Nutritional Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109887 - 109887

Published: Feb. 1, 2025

Language: Английский

Therapeutic potential of tryptophan metabolite indoleacrylic acid in inflammatory bowel disease: From cellular mechanisms to zebrafish stress-like behavior

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 149, P. 114207 - 114207

Published: Feb. 3, 2025

Language: Английский