Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies

Diagnostics, Journal Year: 2024, Volume and Issue: 14(5), P. 519 - 519

Published: Feb. 29, 2024

The urgent need for accurate COVID-19 diagnostics has led to the development of various SARS-CoV-2 detection technologies. Real-time reverse transcriptase polymerase chain reaction (RT-qPCR) remains a reliable viral gene technique, while other molecular methods, including nucleic acid amplification techniques (NAATs) and isothermal techniques, provide diverse effective approaches. Serological assays, detecting antibodies in response infection, are crucial disease surveillance. Saliva-based immunoassays show promise surveillance purposes. efficiency antibody varies, with IgM indicating recent exposure IgG offering prolonged detectability. Various rapid tests, lateral-flow immunoassays, present opportunities quick diagnosis, but their clinical significance requires validation through further studies. Challenges include variations specificity sensitivity among testing platforms evolving assay sensitivities over time. antigens, particularly N S proteins, play role diagnostic methods. Innovative approaches, such as nanozyme-based assays specific nucleotide aptamers, offer enhanced flexibility. In conclusion, ongoing advancements methods contribute global effort combating pandemic.

Language: Английский

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Molecular diagnostics: past, present, and future

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 3 - 23

Published: Jan. 1, 2024

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Serologic LSPR-nanosensor against SARS-COV-2 antibodies and related variants outperforms ELISA in sensitivity

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: March 3, 2025

Language: Английский

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Peptide-Coated Nanoparticles for Noninvasive Biomedical Imaging

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 37 - 53

Published: Jan. 1, 2025

Language: Английский

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Advanced Computational Techniques for Plasmonic Metasurfaces in the Detection of Neglected Infectious Diseases

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

This tutorial delves into the integration of plasmonic metasurfaces as cutting-edge tools for creating highly sensitive diagnostic assays tailored to neglected infectious diseases. Plasmonic provide a transformative approach diagnostics by addressing common limitations traditional methods, including slow results and high costs. explores their application in advancing cost-effective solutions manuscript covers complete cycle developing optimized, AI-driven metasurfaces, from biofunctionalization strategies advanced fabrication techniques scalability, regulatory challenges, point-of-care accessibility resource-limited settings.

Language: Английский

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Lyophilized nasal swabs for COVID-19 detection by ATR-FTIR spectroscopy: Machine learning-based approach

Biophysical Chemistry, Journal Year: 2025, Volume and Issue: 324, P. 107459 - 107459

Published: May 14, 2025

Language: Английский

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Fluorescence Signal Enhancement-Based On-Site Single-Molecule Sensing: A Review

IEEE Sensors Journal, Journal Year: 2024, Volume and Issue: 24(13), P. 20322 - 20330

Published: May 27, 2024

Compared to conventional sensing, single-molecule sensing is proven have extremely high sensitivity and has thus been successfully applied in various fields. However, many techniques rely on precise but bulky instruments complicated operations, limiting their scope on-site applications. To solve the problem, we reported a series of works detecting chemicals, proteins, nucleic acids at level. Though these deal with diverse targets (avian influenza virus (AIV), hepatitis B (HBV), mercury ion (Hg ²⁺ )) use different devices, they same tactic, which employs fluorescence-enhanced probes, supporting portable fluorescence microscope equipped an industrial camera collect analyze signal. We optimized operations guarantee that all procedures can be conveniently implemented using field-portable devices. Therefore, based optical platform, our tactic advantages such as sensitivity, accuracy, simple procedures, fast speed. In this paper, describe key components designed including system, operations. compare other approaches discuss pros cons, well prospect sensing. Our will promote speed, convenience, automation pave way for development instruments.

Language: Английский

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Detection of anti-SARS CoV-2 antibodies in human serum by means of Bloch surface waves on 1D photonic crystal biochips

Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 15, P. 100413 - 100413

Published: Nov. 3, 2023

This study presents the development and characterization of a disposable biochip for detection antibodies against SARS-CoV-2 spike protein, well-known target vaccine therapeutic development. is based on one-dimensional photonic crystal (1DPC) deposited plastic substrate designed to sustain Bloch surface waves (BSW) in visible range. The experimental phase was carried out using conjunction with custom-made optical read-out platform capable real-time refractometric fluorescence-based end-point measurements. Our functionalized by immobilizing receptor-binding domain protein onto silanization process. Human serum samples, including negative control positive sample from recovered COVID-19 patient, were tested biochip. results show that discriminates between samples label-free mode down 1:10 dilution sera quantum dot amplified fluorescence 1:100 dilution. demonstrate potential sensitive specific antibodies.

Language: Английский

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Discrimination between Covid-19 positive and negative blood serum based on excitation-emission matrix fluorescence spectroscopy and chemometrics

Talanta, Journal Year: 2024, Volume and Issue: 280, P. 126788 - 126788

Published: Aug. 28, 2024

Language: Английский

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CRISPR for companion diagnostics in low-resource settings

Lab on a Chip, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

New point-of-care tests (POCTs), which are especially useful in low-resource settings, needed to expand screening capacity for diseases that cause significant mortality: tuberculosis, multiple cancers, and emerging infectious diseases. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic (CRISPR-Dx) assays have emerged as powerful versatile alternatives traditional nucleic acid tests, revealing a strong potential meet this need new POCTs. In review, we discuss CRISPR-Dx assay techniques been or could be applied develop POCTs, including sample processing, target amplification, multiplex design, signal readout. This review also describes current applications POCTs disease diagnosis includes future opportunities challenges such tests. These advance beyond initial development efforts broadly criteria use settings.

Language: Английский

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