Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,
Journal Year:
2024,
Volume and Issue:
43(6), P. 153 - 159
Published: Dec. 1, 2024
Congenital
hypothyroidism
(CH)
is
a
major
health
issue
that
can
lead
to
intellectual
disability
if
not
detected
and
treated
earlier.
The
preliminary
screening
program
for
neonatal
CH
in
Indonesia
gave
provisional
incidence
of
1:2513.
Newborn
using
dried
blood
spot
sample
the
standard
method
detection,
but
it
has
limitations.
Despite
proven
benefits
screening,
still
faces
significant
challenges
implementing
nationwide
program.
This
study
aimed
develop
more
sensitive
accessible
by
creating
monoclonal
antibodies
(mAbs)
against
thyroid-stimulating
hormone
(TSH).TSH
protein
was
isolated
from
newborn
cord
confirmed
Western
blot
analysis.
Mice
were
immunized
with
purified
TSH,
hybridoma
cell
lines
generated
through
fusion.
Hybridoma
supernatants
screened
TSH-specific
ELISA.
mAb
highest
titer
dialysis.
analysis
presence
TSH
fraction
at
28
kDa.
Immunized
mice
showed
increase
antibody
compared
control
group.
clones
secreting
high-titer
identified.
research
successfully
produced
mAbs
it.
They
enable
development
rapid,
point-of-care
diagnostic
tests,
such
as
lateral
flow
immunoassays,
which
provide
results
within
minutes.
It
will
lay
groundwork
innovative
tools
significantly
improve
early
diagnosis
treatment
this
condition,
particularly
resource-limited
settings.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Nov. 18, 2024
Since
the
outbreak
of
new
coronavirus,
point-of-care
diagnostics
based
on
nucleic
acid
testing
have
become
a
requirement
for
development
pathogen
diagnostics,
which
require
ability
to
accurately,
rapidly,
and
conveniently
detect
pathogens.
Conventional
amplification
techniques
no
longer
meet
requirements
detection
in
low-resource,
low-skill
environments
because
they
specialist
equipment,
complex
operations,
long
times.
Therefore,
recombinant
polymerase
(RPA)
is
becoming
an
increasingly
important
method
today’s
technology
it
can
amplify
acids
20–30
minutes
at
constant
temperature,
greatly
reducing
dependence
equipment
technicians.
RPA
products
are
primarily
detected
through
methods
such
as
real-time
fluorescence,
gel
electrophoresis,
lateral
flow
assays
(LFAs),
other
techniques.
Among
these,
LFAs
allow
rapid
within
visualization
results,
offering
convenient
operation
low
cost.
combination
with
LFA
has
significant
advantages
holds
broad
application
prospects
(POC)
particularly
low-resource
settings.
Here,
we
focus
principles
combined
LFAs,
their
diagnosis,
main
limitations,
some
improvements
methods.
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,
Journal Year:
2024,
Volume and Issue:
43(6), P. 153 - 159
Published: Dec. 1, 2024
Congenital
hypothyroidism
(CH)
is
a
major
health
issue
that
can
lead
to
intellectual
disability
if
not
detected
and
treated
earlier.
The
preliminary
screening
program
for
neonatal
CH
in
Indonesia
gave
provisional
incidence
of
1:2513.
Newborn
using
dried
blood
spot
sample
the
standard
method
detection,
but
it
has
limitations.
Despite
proven
benefits
screening,
still
faces
significant
challenges
implementing
nationwide
program.
This
study
aimed
develop
more
sensitive
accessible
by
creating
monoclonal
antibodies
(mAbs)
against
thyroid-stimulating
hormone
(TSH).TSH
protein
was
isolated
from
newborn
cord
confirmed
Western
blot
analysis.
Mice
were
immunized
with
purified
TSH,
hybridoma
cell
lines
generated
through
fusion.
Hybridoma
supernatants
screened
TSH-specific
ELISA.
mAb
highest
titer
dialysis.
analysis
presence
TSH
fraction
at
28
kDa.
Immunized
mice
showed
increase
antibody
compared
control
group.
clones
secreting
high-titer
identified.
research
successfully
produced
mAbs
it.
They
enable
development
rapid,
point-of-care
diagnostic
tests,
such
as
lateral
flow
immunoassays,
which
provide
results
within
minutes.
It
will
lay
groundwork
innovative
tools
significantly
improve
early
diagnosis
treatment
this
condition,
particularly
resource-limited
settings.
