Український радіологічний та онкологічний журнал,
Journal Year:
2024,
Volume and Issue:
32(4), P. 504 - 517
Published: Dec. 3, 2024
Background.
Lung
cancer
continues
to
be
a
significant
health
concern
globally.
Due
the
heterogeneity
of
disease,
using
innovative
strategies
for
effective
management
and
treatment
patients
is
extremely
important.
Purpose
–
characterize
mutational
profile
group
non-small
cell
lung
(NSCLC)
utilizing
next-generation
sequencing
technique.
Materials
Methods.
A
total
42
samples
that
were
fixed
in
formalin
embedded
paraffin
(FFPE)
collected
from
Ukrainian
diagnosed
with
who
had
surgery
at
Sumy
Regional
Clinical
Oncology
Center.
DNA
was
extracted
FFPE
Omega
Bio-tek
E.Z.N.A.®
Kit
(USA)
following
manufacturerʼs
instructions.
Sequencing
performed
on
Illumina
NextSeq
550Dx
platform
550
Mid-Output
Kit.
The
Cancer
Genome
Atlas
Program
(TCGA)
database
(https://portal.gdc.cancer.gov/)
used
comparative
analysis
prevalence
genomic
mutations
cohort
Caucasian
NSCLC.
Statistical
Stata
V.18.0
software
(StataCorp,
Texas,
USA;
https://www.stata.com;
2024).
paper
belongs
«description
case
series»
category
which
type
study
recognized
by
evidence
based
medicine
does
not
claim
statistical
significance
result.
Results.
Among
NSCLC
samples,
11
(26.19%)
carried
driver
such
as
EGFR
(n=2;
L858R),
KRAS
(n=7;
G12C,
G12D,
G12A
A146S),
BRAF
(n=1;
V600E)
translocation
EML4(exon6)
ALK
(exon20)
chr2:42503838
chr2:29447579).
All
mutually
exclusive.
No
NRAS,
ROS1,
RET,
MET,
ERBB2,
PIK3CA
mutation
cases
detected.
number
never
smoked
significantly
higher
than
former
or
current
smokers
(p=0.046).
association
found
between
age,
sex,
tumor
stage,
histology
NSCLC,
mutations.
Conclusions.
Molecular
genetic
profiling
revealed
26.19%
radically
treated
Most
are
oncogenic
sensitive
tyrosine
kinase
inhibitors.
Australasian Journal of Dermatology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 17, 2025
ABSTRACT
Advances
in
genetic
sequencing
technologies
have
enabled
the
identification
of
key
activating
somatic
variants
cellular
signalling
pathways
involved
pathogenesis
vascular
malformations.
Given
that
these
are
also
implicated
several
cancers,
repurposing
targeted
therapies
developed
oncology
has
been
increasingly
investigated
for
treating
This
review
provides
an
update
on
current
evidence
slow‐flow
malformations,
particularly
context
gain‐of‐function
PI3K/AKT/mTOR
pathway.
Exploration of Immunology,
Journal Year:
2025,
Volume and Issue:
5
Published: April 8, 2025
Neoantigen
vaccines
are
a
promising
strategy
in
cancer
immunotherapy
that
leverage
tumor-specific
mutations
to
elicit
targeted
immune
responses.
Although
they
have
considerable
potential,
development
challenges
related
antigen
prediction
accuracy,
manufacturing
complexity,
and
scalability
remain
key
obstacles
their
widespread
clinical
use.
This
literature
review
was
conducted
using
PubMed,
Scopus,
Web
of
Science,
Google
Scholar
databases
identify
relevant
studies.
Keywords
included
“neoantigen
vaccines,”
“personalized
immunotherapy,”
“tumor
heterogeneity,”
“bioinformatics
pipelines,”
“prediction
algorithms”.
Clinical
trial
data
were
sourced
from
ClinicalTrials.gov,
Trialtrove,
other
publicly
available
registries.
Eligible
studies
peer-reviewed
research
articles,
systematic
reviews,
trials
focusing
on
neoantigen
vaccine
development,
bioinformatic
strategies,
immunotherapy.
Tumor
heterogeneity
clonal
evolution
significantly
impact
efficacy,
necessitating
multi-epitope
targeting
adaptive
design.
Current
algorithms
suffer
high
false-positive
false-negative
rates,
requiring
further
integration
with
multi-omics
machine
learning
enhance
accuracy.
Manufacturing
remains
complex,
time-intensive,
costly,
advancements
standardization
automation.
Combination
therapies,
such
as
checkpoint
inhibitors
adoptive
cell
counteract
the
immunosuppressive
tumor
microenvironment,
improving
treatment
outcomes.
hold
great
potential
for
personalized
therapy
but
require
bioinformatics,
scalability,
immunomodulatory
strategies
efficacy.
Continued
interdisciplinary
collaboration
essential
refining
applications.
Biotechnology
and
genetic
research
in
coldwater
fishes
have
emerged
as
pivotal
fields
of
study
globally,
aiming
to
unlock
the
vast
potential
these
aquatic
species.
The
utilization
biotechnological
tools,
such
engineering,
selective
breeding,
genomic
sequencing,
has
facilitated
remarkable
advancements
understanding
makeup,
physiological
traits,
disease
resistance
mechanisms
fish.
These
breakthroughs
not
only
improved
aquaculture
practices
but
also
contributed
enhancing
fish
health,
growth
rates,
nutritional
quality.
Moreover,
application
biotechnology
extends
environmental
conservation,
addressing
challenges
posed
by
climate
change,
habitat
degradation,
outbreaks.
By
deciphering
resilience
species,
scientists
aim
develop
strategies
for
preserving
biodiversity,
restoring
ecosystems,
ensuring
sustainable
fisheries
management.
However,
ethical
considerations,
regulatory
frameworks,
public
perceptions
remain
significant
factors
influencing
implementation
research.
Balancing
scientific
innovation
with
standards
societal
concerns
is
essential
navigate
complex
landscape
manipulation
interventions.
Looking
ahead,
future
holds
promise
further
productivity,
food
security,
conserving
fragile
ecosystems.
Collaborative
efforts
among
scientists,
policymakers,
industry
stakeholders,
communities
are
imperative
harness
full
while
ethical,
environmental,
implications
development
resources
worldwide.
Ayush journal of integrative oncology.,
Journal Year:
2024,
Volume and Issue:
1(1&2), P. 10 - 12
Published: Jan. 1, 2024
Abstract
Background:
Ayurveda,
traditionally
known
as
“The
Science
of
Life,”
has
been
practiced
in
India
for
centuries,
focusing
on
holistic
well-being.
Despite
its
longstanding
application,
Ayurveda
faced
challenges
gaining
scientific
acceptance
globally,
primarily
due
to
the
absence
standardized
drug
testing
and
certification.
Objective:
This
study
explores
recent
initiatives
by
Indian
government
globalize
integrating
modern
molecular
biology
techniques
high-throughput
technologies
provide
a
foundation
Ayurvedic
practices.
Methods:
The
research
examines
integration
genomics
proteomics
with
giving
rise
Ayurgenomics
Ayurproteomics.
These
emerging
fields
aim
elucidate
mechanisms
underlying
practices,
leveraging
contemporary
tools.
Results:
interdisciplinary
approach
seeks
generate
evidence-based
data
diagnostics
therapeutics,
explore
pharmacokinetics
pharmacodynamics,
ensure
safety
efficacy
medicines.
Additionally,
highlights
need
validation,
ethical
guidelines,
collaboration
between
traditional
practitioners
scientists.
Conclusion:
Integrating
like
potential
validate
practices
lead
development
personalized
medicine.
Overcoming
validation
promoting
collaborative
are
key
success
Ayurproteomics
healthcare.
