Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: May 27, 2024
Notch
signaling,
renowned
for
its
role
in
regulating
cell
fate,
organ
development,
and
tissue
homeostasis
across
metazoans,
is
highly
conserved
throughout
evolution.
The
receptor
ligands
are
transmembrane
proteins
containing
epidermal
growth
factor-like
repeat
sequences,
typically
necessitating
receptor-ligand
interaction
to
initiate
classical
signaling
transduction.
Accumulating
evidence
indicates
that
the
pathway
serves
as
both
an
oncogenic
factor
a
tumor
suppressor
various
cancer
types.
Dysregulation
of
this
promotes
epithelial-mesenchymal
transition
angiogenesis
malignancies,
closely
linked
proliferation,
invasion,
metastasis.
Furthermore,
contributes
maintaining
stem-like
properties
cells,
thereby
enhancing
invasiveness.
regulatory
metabolic
reprogramming
microenvironment
suggests
pivotal
involvement
balancing
suppressive
effects.
Moreover,
implicated
conferring
chemoresistance
cells.
Therefore,
comprehensive
understanding
these
biological
processes
crucial
developing
innovative
therapeutic
strategies
targeting
signaling.
This
review
focuses
on
research
progress
cancers,
providing
in-depth
insights
into
potential
mechanisms
regulation
occurrence
progression
cancer.
Additionally,
summarizes
pharmaceutical
clinical
trials
therapy,
aiming
offer
new
human
malignancies.
Journal of Translational Medicine,
Journal Year:
2023,
Volume and Issue:
21(1)
Published: May 18, 2023
Spatial
transcriptomics
technologies
developed
in
recent
years
can
provide
various
information
including
tissue
heterogeneity,
which
is
fundamental
biological
and
medical
research,
have
been
making
significant
breakthroughs.
Single-cell
RNA
sequencing
(scRNA-seq)
cannot
spatial
information,
while
allow
gene
expression
to
be
obtained
from
intact
sections
the
original
physiological
context
at
a
resolution.
Various
insights
generated
into
architecture
further
elucidation
of
interaction
between
cells
microenvironment.
Thus,
we
gain
general
understanding
histogenesis
processes
disease
pathogenesis,
etc.
Furthermore,
silico
methods
involving
widely
distributed
R
Python
packages
for
data
analysis
play
essential
roles
deriving
indispensable
bioinformation
eliminating
technological
limitations.
In
this
review,
summarize
available
transcriptomics,
probe
several
applications,
discuss
computational
strategies
raise
future
perspectives,
highlighting
developmental
potential.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Oct. 9, 2023
Tissues
are
complex
environments
where
different
cell
types
in
constant
interaction
with
each
other
and
non-cellular
components.
Preserving
the
spatial
context
during
proteomics
analyses
of
tissue
samples
has
become
an
important
objective
for
applications,
one
most
being
investigation
tumor
microenvironment.
Here,
we
describe
a
multiplexed
protein
biomarker
detection
method
on
COMET
instrument,
coined
sequential
ImmunoFluorescence
(seqIF).
The
fully
automated
uses
successive
applications
antibody
incubation
elution,
in-situ
imaging
enabled
by
integrated
microscope
microfluidic
chip
that
provides
optimized
optical
access
to
sample.
We
show
seqIF
data
sample
such
as
healthy
tissue,
including
40-plex
single
section
is
obtained
less
than
24
h,
using
off-the-shelf
antibodies.
also
present
extensive
characterization
developed
method,
elution
efficiency,
epitope
stability,
repeatability
reproducibility,
signal
uniformity,
dynamic
range,
addition
marker
panel
optimization
strategies.
streamlined
workflow
antibodies,
quality
enabling
downstream
analysis,
ease
reaching
hyperplex
levels
make
suitable
immune-oncology
research
disciplines
requiring
paving
way
its
adoption
clinical
settings.
Cell Death and Disease,
Journal Year:
2024,
Volume and Issue:
15(4)
Published: April 1, 2024
Abstract
Macrophages,
as
pivotal
cells
within
the
tumour
microenvironment,
significantly
influence
impact
of
and
reactions
to
treatments
for
solid
tumours.
The
rapid
evolution
bioengineering
technology
has
revealed
vast
potential
engineered
macrophages
in
immunotherapy,
disease
diagnosis,
tissue
engineering.
Given
this
landscape,
goal
harnessing
innovating
a
novel
strategy
immunotherapy
cannot
be
overstated.
diverse
strategies
realm
cancer
encompassing
macrophage
drug
delivery
systems,
chimeric
antigen
receptor
therapy,
synergistic
treatment
approaches
involving
bacterial
outer
membrane
vesicles
macrophages,
are
meticulously
examined
review.
These
methodologies
designed
enhance
therapeutic
efficacy
against
tumours,
particularly
those
that
drug-resistant
metastatic.
Collectively,
these
immunotherapies
poised
supplement
refine
current
paradigms,
thus
heralding
new
frontier
fight
malignant
Cancer Research,
Journal Year:
2024,
Volume and Issue:
84(8), P. 1210 - 1220
Published: Feb. 5, 2024
Abstract
The
tumor
microenvironment
(TME)
represents
a
complex
network
in
which
cells
communicate
not
only
with
each
other
but
also
stromal
and
immune
cells.
intercellular
interactions
the
TME
contribute
to
initiation,
progression,
metastasis,
treatment
outcome.
Recent
advances
spatial
transcriptomics
(ST)
have
revolutionized
molecular
understanding
of
at
level.
A
comprehensive
interactive
analysis
resource
specifically
designed
for
characterizing
could
facilitate
further
using
ST.
In
this
study,
we
collected
296
ST
slides
covering
19
cancer
types
developed
computational
pipeline
delineate
structure
along
malignant–boundary–nonmalignant
axis.
identified
differentially
expressed
genes
their
functional
enrichment,
deconvoluted
cellular
composition
TME,
reconstructed
cell
type–specific
gene
expression
profiles
sub-spot
level,
performed
cell–cell
interaction
analysis.
Finally,
user-friendly
database
SpatialTME
(http://www.spatialtme.yelab.site/)
was
constructed
provide
search,
visualization,
downloadable
results.
These
detailed
analyses
are
able
reveal
heterogeneous
regulatory
elucidate
associations
between
features
development
or
response
therapy,
offering
valuable
study
TME.
Significance:
provides
structure,
composition,
expression,
function,
information
enable
investigations
into
level
advance
treatment.
