2‐dodecyl‐6‐methoxycyclohexa‐2,5‐diene‐1,4‐dione mediates the effect of ROS‐enhanced PI3K/Akt/mTOR pathway on autophagy in breast cancer DOI Creative Commons
Liang Chen, Meifeng Chen, Yan Xie

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Several studies have suggested a potential antitumor effect of 2‐dodecyl‐6‐methoxycyclohexa‐2,5‐diene‐1,4‐dione (DMDD). To further understand the mechanism action this compound, we investigated its on phosphatidylinositol‐3‐kinase (PI3K)/serine–threonine kinase (Akt)/mammalian target rapamycin (mTOR) signaling pathway. We show that DMDD application significantly inhibited proliferation breast cancer cell lines MDA‐MB‐231 and ER‐α positive MCF‐7. Furthermore, resulted in increased intracellular reactive oxygen species (ROS) levels, apoptosis autophagy, whereas it downregulated expression PI3K, Akt mTOR mRNA proteins, LC3II/I p62 proteins. In mouse xenograft model, tumor growth. Expression analyses suggest ROS levels were higher treated tissues, immunohistochemical apoptotic cells more prevalent group compared to control group. Taken together, our results molecular may involve enhancement autophagy through PI3K/Akt/mTOR pathway by mediating expression.

Language: Английский

2‐dodecyl‐6‐methoxycyclohexa‐2,5‐diene‐1,4‐dione mediates the effect of ROS‐enhanced PI3K/Akt/mTOR pathway on autophagy in breast cancer DOI Creative Commons
Liang Chen, Meifeng Chen, Yan Xie

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Several studies have suggested a potential antitumor effect of 2‐dodecyl‐6‐methoxycyclohexa‐2,5‐diene‐1,4‐dione (DMDD). To further understand the mechanism action this compound, we investigated its on phosphatidylinositol‐3‐kinase (PI3K)/serine–threonine kinase (Akt)/mammalian target rapamycin (mTOR) signaling pathway. We show that DMDD application significantly inhibited proliferation breast cancer cell lines MDA‐MB‐231 and ER‐α positive MCF‐7. Furthermore, resulted in increased intracellular reactive oxygen species (ROS) levels, apoptosis autophagy, whereas it downregulated expression PI3K, Akt mTOR mRNA proteins, LC3II/I p62 proteins. In mouse xenograft model, tumor growth. Expression analyses suggest ROS levels were higher treated tissues, immunohistochemical apoptotic cells more prevalent group compared to control group. Taken together, our results molecular may involve enhancement autophagy through PI3K/Akt/mTOR pathway by mediating expression.

Language: Английский

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