Cyclic Enrichment of Urinary Exosomes Using a MOF‐on‐MOF‐Based Asymmetric Impinging Streams Chip for Bladder Cancer Diagnosis and Prognosis Prediction DOI
Min Liu,

Gaihua Jia,

Xiangyu Meng

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Urinary exosomes are valuable biomarkers for the liquid biopsy of bladder cancer (BCa), carrying critical biomolecules associated with BCa that assist in clinical diagnosis and prognosis prediction. However, lack rapid, efficient, high‐throughput techniques urinary exosome enrichment limits their application. This study presents an cyclic chip based on asymmetric impinging streams a metal‐organic framework (MOF)‐on‐MOF material. The newly synthesized MOF material exhibited excellent exosome‐enrichment capabilities. Moreover, integration fluidic pathway within notably enhanced collision efficiency between MOF. Leveraging chip, enables rapid efficient from 10 mL urine sample 30 min, without requirement pre‐concentration. In 300 subjects two centers, diagnostic model exosomal NORAD (Exo‐NORAD) sediment (Sed‐NORAD) is developed, which showed higher detection positivity rate than fluorescence situ hybridization (FISH) cytology. Exo‐NORAD identified as independent prognostic factor using Cox regression analysis. conclusion, this provides new approach noninvasive prediction BCa.

Language: Английский

Cyclic Enrichment of Urinary Exosomes Using a MOF‐on‐MOF‐Based Asymmetric Impinging Streams Chip for Bladder Cancer Diagnosis and Prognosis Prediction DOI
Min Liu,

Gaihua Jia,

Xiangyu Meng

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Urinary exosomes are valuable biomarkers for the liquid biopsy of bladder cancer (BCa), carrying critical biomolecules associated with BCa that assist in clinical diagnosis and prognosis prediction. However, lack rapid, efficient, high‐throughput techniques urinary exosome enrichment limits their application. This study presents an cyclic chip based on asymmetric impinging streams a metal‐organic framework (MOF)‐on‐MOF material. The newly synthesized MOF material exhibited excellent exosome‐enrichment capabilities. Moreover, integration fluidic pathway within notably enhanced collision efficiency between MOF. Leveraging chip, enables rapid efficient from 10 mL urine sample 30 min, without requirement pre‐concentration. In 300 subjects two centers, diagnostic model exosomal NORAD (Exo‐NORAD) sediment (Sed‐NORAD) is developed, which showed higher detection positivity rate than fluorescence situ hybridization (FISH) cytology. Exo‐NORAD identified as independent prognostic factor using Cox regression analysis. conclusion, this provides new approach noninvasive prediction BCa.

Language: Английский

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