The current research status of immobilized lipase performance and its potential for application in food are developing toward green and healthy direction: A review

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Abstract Immobilized lipases have received great attention in food, environment, medicine, and other fields due to their easy separation, high stability (temperature, pH), storage properties. After immobilization, lipase transforms from a homogeneous heterogeneous state, making it easier recover the reaction substrate achieve recycling, which is line with concept of green chemistry reduces protein contamination product. There are various materials for enzyme including polysaccharides natural sources, inorganic compounds, carbon nanotubes, metal–organic framework materials, so forth. Magnetic immobilization carriers been widely studied ability separation by adding magnetic field. Its method can be simply divided into two categories: physical action (adsorption, embedding) chemical binding (covalent, cross‐linking). Some studies mainly discuss support methods, applications immobilized food. On this basis, our review also focuses on changes crosslinking agents lipases, different methods promote new trends study proposes prospects research food industry.

Language: Английский

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Stepwise Lighting Up Gold(I)–Thiolate Complexes from AIE Nanoaggregates to AIEE Nanoprobes with a ZIF-8 Shell for Glucose Biosensing

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Aggregation-induced emission (AIE) or aggregation-induced enhancement (AIEE) has endowed gold species with responsive fluorescent properties, favoring their potential applications in sensing, imaging, and therapy. However, it remains an interesting challenge to fabricate fluorophores both AIE AIEE effects. Herein, we presented highly luminescent Au(I) thiolate nanocomplex-based biosensors Zn2+ induced-AIE zeolite imidazolate framework (ZIF-8) induced-AIEE The nonemissive monovalent gold–glutathione complexes (AuI-SGs) were obtained synthesize the core–shell Zn2+/AuI-SG@ZIF-8 composites strong luminescence via coordination-assisted self-assembly strategy. By immobilizing GOx on surface of Zn2+/AuI-SG@ZIF-8, Zn2+/AuI-SG@ZIF-8/GOx exhibited effective responsiveness glucose, showing a "turn-off" detection model. mechanism study revealed that robust glucose sensing was attributed acid-stimulated degradation probe facilitated by H+ generated from oxidase (GOx)-catalyzed oxidation process. To achieve noninvasive intelligent blood detection, Zn2+/AuI-SG@ZIF-8/GOx-loaded microneedle (MN)-patch platform further developed. MN-patch-based had promising performance for on-needle capture situ detection. This demonstrated universal feasible protocol construct development MN-based analytical devices.

Language: Английский

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Insight into the efficient loading and enhanced activity of enzymes immobilized on functionalized UiO-66

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135557 - 135557

Published: Sept. 1, 2024

Language: Английский

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High-Efficiency Enzyme Assay and Screening of Enzyme-Inhibiting Nanomaterials Using Capillary Electrophoresis with Hierarchically Porous Metal–Organic Framework-Based Immobilized Enzyme Microreactor

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17300 - 17309

Published: Oct. 16, 2024

Enzyme-inhibiting nanomaterials have significant potential for regulating enzyme activity. However, a universal and efficient method systematically screening evaluating the inhibitory effects of various on drug target enzymes has not been established. While integrated technique immobilized microreactor (IMER) with capillary electrophoresis (CE) serves as an effective tool analysis, it still faces challenges such low loadability, unsatisfactory stability, limited applicability. Herein, hierarchical porous metal–organic frameworks (HP-MOFs) were explored high-performance immobilization carriers stationary phases to develop novel HP-MOFs-based IMER-CE microanalysis system online assay systematic enzyme-inhibiting nanomaterials. As proof-of-concept demonstration, model xanthine oxidase (XOD) was HP-UiO-66-NH2 coated capillary, serving durable IMER XOD-inhibiting The hierarchically micro- mesoporous structure superior loadability as-prepared HP-UiO-66-NH2–IMER intensively characterized, followed by evaluation separation performance column kinetics XOD. Compared microporous UiO-66-NH2–IMER, HP-UiO-66-NH2–IMER-CE showed improvements in loading, maximum reaction rate, repeatability, long-term stability. Furthermore, established effectively employed screen XOD activity nanomaterials, revealing that graphene oxide, single wall carbon nanotube three other exhibited potentials. can be easily expanded modifying types holds accelerate identification rational design

Language: Английский

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Evaluation of the immobilized enzymes function in soil remediation following polycyclic aromatic hydrocarbon contamination

Environment International, Journal Year: 2024, Volume and Issue: 194, P. 109106 - 109106

Published: Oct. 30, 2024

The bioremediation of polycyclic aromatic hydrocarbon (PAHs) from soil utilizing microorganisms, enzymes, microbial consortiums, strains, etc. has attracted a lot interest due to the environmentally friendly, and cost-effective features. Enzymes can efficiently break down PAHs in by hydroxylating benzene ring, breaking C-C bond, catalyze hydroxylation variety ring compounds via single-electron transfer oxidation. However, practical application is limited its instability ease loss function under harsh environmental conditions such as pH, temperature, edaphic stress Therefore, this paper focused on techniques used immobilize enzymes remediate soil. Moreover, previous research not adequately covered topic, despite employment several immobilized aqueous solution cultures other types organic pollutants. Bibliometric analysis further highlighted trends 2000 2023 field growing identified important challenges regarding enzyme stability interaction with matrices. findings indicated that may catalyzed oxidation OH groups rings, generate benzyl radicals (i.e.,

Language: Английский

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Ionic liquid interface modification of magnetic carboxymethyl cellulose-immobilized lipase: Improvement of lid and tunnel structures to enhance stability and catalytic performance

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160647 - 160647

Published: Feb. 1, 2025

Language: Английский

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Nanogel-dressed Candida rugosa lipase: Enhanced biocatalysis through hierarchically porous metal organic frameworks' integration

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143964 - 143964

Published: May 5, 2025

Language: Английский

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Novel Immobilized Enzyme System Using Hydrophobic Dendritic Mesoporous Silica Nanospheres for Efficient Flavor Ester Production

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Enzymatic synthesis of flavor esters is widely used in the food and industries, but challenges remain improving catalytic efficiency stability biocatalysts. This study evaluates performance a novel biocatalyst, CALB@DMSN-C8, formed by immobilizing Candida antarctica lipase B (CALB) on hydrophobic dendritic mesoporous silica nanospheres (DMSN-C8), for synthesizing esters. The CALB@DMSN-C8 catalyst achieves caproic acid conversion rate 98.5 ± 0.5% just 30 min demonstrates outstanding thermal stability, retaining high over 20 reuse cycles. To our knowledge, this represents most efficient esters, including ethyl valerate, caproate, heptanoate, caprylate, compared to studies existing literature. Analysis aroma characteristics molecular docking simulations revealed typical profiles mechanisms various mellow develops an innovative strategy using self-made immobilized lipases catalyze production with potential applications cosmetics.

Language: Английский

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Construction of γ-Fe2O3 based colorimetric sensing arrays through the phase transformation induced by rare-earth doping for the detection of antibiotics in water environments

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: 13(3), P. 117126 - 117126

Published: May 16, 2025

Language: Английский

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Pickering emulsions stabilized by chitosan-zein-lipase particles for interfacial catalysis

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 316, P. 144473 - 144473

Published: May 21, 2025

Language: Английский

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