Isomer Differentiation by UHPLC‐Q‐Exactive‐Orbitrap MS led to Enhanced Identification of Daphnane Diterpenoids in Daphne tangutica DOI
Kouharu Otsuki, Mi Zhang,

Lingjian Tan

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

ABSTRACT Introduction Liquid chromatography‐mass spectrometry (LC–MS) has enhanced the rapid, accurate analysis of complex plant extracts, eliminating need for extensive isolation. Tandem mass (MS/MS) further enhances this process by providing detailed structural information. However, differentiating isomers remains a challenge due to their minor spectral and differences. Objective This study aimed extend applicability LC–MS/MS identification daphnane diterpenoids, with particular focus on distinguishing functional isomers. Methods LC–MS analyses were performed using an UHPLC‐Q‐Exactive‐Orbitrap MS. The MS conditions optimized in‐source CID HCD modes reference compounds. A qualitative was then conducted extract Daphne tangutica . chemical structures detected diterpenoids estimated analyzing fragmentation patterns in both spectra product ion spectra. These identifications validated isolation comparison in‐house diterpenoid library. Results By optimizing conditions, especially negative mode, it possible accurately distinguish such as yuanhuajine gniditrin. Qualitative D. identified total 28 daphnanes, including seven previously unreported Furthermore, novel geometric isomer gniditrin isolated conducting crude fraction. Conclusion demonstrated that can effectively thereby enhancing daphnanes extracts highlighting its potential powerful tool phytochemical analysis.

Language: Английский

Discovery of structurally intriguing diterpenoids as anti-neuroinflammatory agents from mangrove plant Excoecaria agallocha L. via inhibiting macrophage polarization and inflammasome DOI
Junyi Li,

Mingbin Chen,

Tingting Fu

et al.

Phytochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 114440 - 114440

Published: Feb. 1, 2025

Language: Английский

Citations

1
Rationally Designed Cell Membrane Biomimetic Biosensing Platform for the Binding Analysis of Drugs with Intracellular Kinase Domain of Epidermal Growth Factor Receptor DOI
Xia Liu, Quan Feng, Qi Hu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Biosensing technologies have demonstrated significant potential in exploring the binding of drugs to receptor tyrosine kinases (RTKs). As a typical transmembrane receptor, there are still several shortcomings utilization intracellular kinase domain RTKs, primary action site small-molecule inhibitors, resulting insufficient and unclear sites, which impair efficiency accuracy biosensing. Herein, using epidermal growth factor (EGFR) as an example, we reported biosensing platform based on cell membrane camouflage technology for evaluating EGFR. The azide-functionalized membranes modified through glucose metabolism were reverse-coated onto alkyne-functionalized magnetic nanoparticles via bioorthogonal reaction (CMRMNPs), therefore effectively exposing EGFR without damage. To construct platform, fluorescent probe derived from gefitinib pharmacophore (GN probe) was further synthesized incubated with CMRMNPs. This strategy facilitated efficient localization GN within Ultimately, this approach successfully implemented evaluate three inhibitors study provides viable constructing biomimetic biosensors defined orientation offers novel insights methodologies drug regions RTKs.

Language: Английский

Citations

0
Antimicrobial diterpenoids from Rosmarinus officinalis DOI

Qingjiang Xu,

Jiachen Liu, Jia Xu

et al.

Steroids, Journal Year: 2025, Volume and Issue: unknown, P. 109589 - 109589

Published: March 1, 2025

Language: Английский

Citations

0
Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi DOI Creative Commons
Bin Ma,

Yimiao Li,

Ting‐Hui Wang

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 350 - 350

Published: May 1, 2025

As an important class of microorganisms, filamentous fungi have crucial roles in protein secretion, secondary metabolite production and environmental pollution control. However, characteristics such as apical growth, heterokaryon, low homologous recombination (HR) efficiency the scarcity genetic markers mean that application traditional gene editing technology faces great challenges. The introduction RNA-mediated CRISPR/Cas (clustered regularly interspaced short palindromic repeat/CRlSPR-associated protein) system recent years has revolutionized fungi. In addition, continuously expressed CRISPR significantly improved efficiency, while optimized sgRNA design reduced cas9 concentration effectively off-target effect, further enhancing safety reliability technology. this review, we systematically analyze molecular mechanism regulatory factors CRISPR/Cas9, focus on optimization its expression improvement transformation fungi, reveal core HR non-homologous end-joining (NHEJ) pathways editing. Based analysis various applications, review reveals outstanding advantages CRISPR/Cas9 enhancement addresses reconstruction metabolic pollutant degradation past decade, provides a theoretical basis practical guidance for engineering applications.

Language: Английский

Citations

0
Qualitative Analysis of Daphnane Diterpenoids in Various Parts of Daphne pontica L. by UHPLC‐Q‐Exactive‐Orbitrap MS DOI
Alev Önder, Kouharu Otsuki, Mi Zhang

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Daphne pontica L. is an evergreen shrub that recorded as anti-diarrheic plant in Turkish folk medicine. Previous studies on D. have reported, albeit slightly, the isolation of daphnane diterpenoids, but no systematic phytochemical analysis diterpenoids has been conducted.

Language: Английский

Citations

1
Anti-HIV diterpenoids from Daphne pseudomezereum DOI
Kouharu Otsuki,

Chihiro Hosoya,

Riko Takamiya

et al.

Phytochemistry, Journal Year: 2024, Volume and Issue: unknown, P. 114366 - 114366

Published: Dec. 1, 2024

Language: Английский

Citations

1
Isomer Differentiation by UHPLC‐Q‐Exactive‐Orbitrap MS led to Enhanced Identification of Daphnane Diterpenoids in Daphne tangutica DOI
Kouharu Otsuki, Mi Zhang,

Lingjian Tan

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

ABSTRACT Introduction Liquid chromatography‐mass spectrometry (LC–MS) has enhanced the rapid, accurate analysis of complex plant extracts, eliminating need for extensive isolation. Tandem mass (MS/MS) further enhances this process by providing detailed structural information. However, differentiating isomers remains a challenge due to their minor spectral and differences. Objective This study aimed extend applicability LC–MS/MS identification daphnane diterpenoids, with particular focus on distinguishing functional isomers. Methods LC–MS analyses were performed using an UHPLC‐Q‐Exactive‐Orbitrap MS. The MS conditions optimized in‐source CID HCD modes reference compounds. A qualitative was then conducted extract Daphne tangutica . chemical structures detected diterpenoids estimated analyzing fragmentation patterns in both spectra product ion spectra. These identifications validated isolation comparison in‐house diterpenoid library. Results By optimizing conditions, especially negative mode, it possible accurately distinguish such as yuanhuajine gniditrin. Qualitative D. identified total 28 daphnanes, including seven previously unreported Furthermore, novel geometric isomer gniditrin isolated conducting crude fraction. Conclusion demonstrated that can effectively thereby enhancing daphnanes extracts highlighting its potential powerful tool phytochemical analysis.

Language: Английский

Citations

0