A Biochemical Deconstruction-Based Strategy to Assist the Characterization of Bacterial Electric Conductive Filaments DOI Open Access
Marta A. Silva, Ana P. Fernandes, David L. Turner

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7032 - 7032

Published: April 11, 2023

Periplasmic nanowires and electric conductive filaments made of the polymeric assembly c-type cytochromes from Geobacter sulfurreducens bacterium are crucial for electron storage and/or extracellular transfer. The elucidation redox properties each heme is fundamental to understanding transfer mechanisms in these systems, which first requires specific assignment NMR signals. high number hemes molecular weight dramatically decrease spectral resolution make this extremely complex or unattainable. nanowire cytochrome GSU1996 (~42 kDa) composed four domains (A D) containing three groups. In work, individual D), bi-domains (AB, CD) full-length were separately produced at natural abundance. Sufficient protein expression was obtained C (~11 kDa/three hemes) D (~10 hemes), as well bi-domain CD (~21 kDa/six hemes). Using 2D-NMR experiments, proton signals then used guide corresponding hexaheme CD. This new biochemical deconstruction-based procedure, using a model, establishes strategy functionally characterize large multiheme cytochromes.

Language: Английский

Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE DOI Creative Commons
Pilar C. Portela, Catharine Shipps, Cong Shen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 20, 2024

Abstract Extracellular electron transfer (EET) via microbial nanowires drives globally-important environmental processes and biotechnological applications for bioenergy, bioremediation, bioelectronics. Due to highly-redundant complex EET pathways, it is unclear how microbes wire electrons rapidly (>10 6 s −1 ) from the inner-membrane through outer-surface directly an external environment despite a crowded periplasm slow (<10 5 diffusion among periplasmic cytochromes. Here, we show that Geobacter sulfurreducens cytochromes PpcABCDE inject into OmcS by binding transiently with differing efficiencies, least-abundant cytochrome (PpcC) showing highest efficiency. Remarkably, this defined nanowire-charging pathway evolutionarily conserved in phylogenetically-diverse bacteria capable of EET. heme reduction potentials are within 200 mV each other, midpoint 82 mV-higher than reported previously. This could explain efficient over micrometres at ultrafast (<200 fs) rates negligible energy loss. Engineering minimal may yield chassis improved performance.

Language: Английский

Citations

24
Recent trends, perceptions and applications of microbial bioelectrochemical systems: An innovative technology DOI

Fatima Akram,

Deborah Salamat,

Taseer Fatima

et al.

Journal of Electroanalytical Chemistry, Journal Year: 2025, Volume and Issue: 979, P. 118933 - 118933

Published: Jan. 9, 2025

Language: Английский

Citations

2
A review of application of combined biochar and iron-based materials in anaerobic digestion for enhancing biogas productivity: Mechanisms, approaches and performance DOI

Likui Feng,

Zhelu Gao,

Tianyi Hu

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 234, P. 116589 - 116589

Published: July 7, 2023

Language: Английский

Citations

28
Molecular Mechanisms of Microbial Extracellular Electron Transfer: The Importance of Multiheme Cytochromes DOI Creative Commons
Catarina M. Paquete, Leonor Morgado, Carlos A. Salgueiro

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2022, Volume and Issue: 27(6)

Published: June 1, 2022

Extracellular electron transfer is a key metabolic process of many organisms that enables them to exchange electrons with extracellular donors/acceptors. The discovery these abilities and the understanding their processes has become priority for scientific industrial community, given growing interest on use in sustainable biotechnological processes. For example, bioelectrochemical systems electrochemical active can an electrode, allowing production energy added-value compounds, among other In systems, electrode through direct or indirect mechanisms, using, most cases, multiheme cytochromes. numerous electroactive organisms, proteins form conductive pathway allows produced from cellular metabolism be transferred across cell surface reduction vice-versa. Here, mechanisms by which promising bacteria perform will reviewed, emphasizing involved pathways. ability some bidirectional pathways used also highlighted.

Language: Английский

Citations

35
Roles of zero-valent iron in anaerobic digestion: Mechanisms, advances and perspectives DOI

Zhang-Wei He,

Zheng-Shuo Zou,

Yong‐Xiang Ren

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 852, P. 158420 - 158420

Published: Aug. 30, 2022

Language: Английский

Citations

31
Physiological potential and evolutionary trajectories of syntrophic sulfate-reducing bacterial partners of anaerobic methanotrophic archaea DOI Creative Commons
Ranjani Murali, Hang Yu, Daan R. Speth

et al.

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(9), P. e3002292 - e3002292

Published: Sept. 25, 2023

Sulfate-coupled anaerobic oxidation of methane (AOM) is performed by multicellular consortia methanotrophic archaea (ANME) in obligate syntrophic partnership with sulfate-reducing bacteria (SRB). Diverse ANME and SRB clades co-associate but the physiological basis for their adaptation diversification not well understood. In this work, we used comparative metagenomics phylogenetics to investigate metabolic among 4 main (HotSeep-1, Seep-SRB2, Seep-SRB1a, Seep-SRB1g) identified features associated lifestyle that distinguish them from non-syntrophic evolutionary neighbors phylum Desulfobacterota. We show protein complexes involved direct interspecies electron transfer (DIET) outer membrane are conserved between lineages. contrast, proteins within inner differ clades, indicative convergent evolution a lifestyle. Our analysis suggests most cases, likely occurred after acquisition DIET an ancestral clade involve horizontal gene transfers pathways (CbcBA) biofilm formation (Pel). also provide evidence unique adaptations which vary depending on archaeal partner. Among widespread SRB, subclades specifically partner ANME-2a missing cobalamin synthesis pathway, suggestive nutritional dependency its partner, while closely related Seep-SRB1a partners ANME-2c lack auxotrophies. work provides insight into DIET-based syntrophy towards it.

Language: Английский

Citations

19
Dynamic pulse approach to enhancing mainstream Anammox process stability: Integrating sidestream support and tackling nitrite-oxidizing bacteria challenges DOI Creative Commons

Yanxiao Wei,

Yujie Chen,

Weizhe Xia

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 395, P. 130327 - 130327

Published: Jan. 17, 2024

Nitrite-oxidizing bacteria (NOB) seriously threaten the partial nitritation and Anammox (PN/A) process, hindering its mainstream application. Herein, a one-stage PN/A reactor was continuously operated for 245 days under nitrogen loading rate lifted from 0.4 g N/L/d to 0.6 0.8 with removal efficiency of 71 %, 64 41 respectively. Furthermore, NOB species over time identified as Nitrospira_sp._OLB3, exhibiting an increase relative abundance 0.9 % 4.3 %. The hydroxyapatite (HAP) granules gradually lost their microbiological function then aged, leading dominance. Therefore, one "pulse therapy" introduced combined "continuous enhancement" sludge supported by sidestream competitively limit dynamics. treatment's effect persisted around two months. strategy that returning at least 50 impaired HAP granular recultivation could fulfill bottlenecks PN/A.

Language: Английский

Citations

5
Competitive bio-augmentation overcoming unusual direct inhibitor inefficacy in mainstream nitrite-oxidizing bacteria suppression: Unveiling the underpinnings in microbial and nitrogen metabolism aspects DOI Creative Commons

Yanxiao Wei,

Min Ye, Yujie Chen

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 171900 - 171900

Published: March 24, 2024

The long-stabilized mainstream partial nitritation/Anammox (PN/A) process continues to encounter significant challenges from nitrite-oxidizing bacteria (NOB). Therefore, this study aimed determine an efficient, rapid, and easily implementable strategy for inhibiting NOB. A laboratory-scale reactor was operated continuously 325 days, experiencing NOB outbreak in recovery with simulated sidestream support. results show that direct inhibitory strategies including intermittent aeration approximately 35 mg/L free ammonia had unusual weak effects on activity. Subsequently, the exogenous Anammox employed as a competitive bio-augmentation approach rapidly inhibited dynamics. Evidence suggests damaged hydroxyapatite granules under low pH conditions might have contributed dominance by diminishing activity, thereby creating substrate-rich environment favoring survival. In contrast, introduction of Candidatus Kuenenia facilitated nitrogen removal efficiency 32.5 % over 80 %. This coincided decrease relative abundance Nitrospira 16.5 2.7 activity 0.34 0.07 g N/(g mixed liquor volatile suspended solid)/d. Metagenomic analysis reveals functional potential most nitrite transport proteins, coupled increase eukaryotic-like serine/threonine-protein kinase involved cellular regulation, during recovery. study's findings reveal feasibility based substrate competition, wherein processes support PN/A integration, offering practical applications.

Language: Английский

Citations

5
Comparison of cable bacteria genera reveals details of their conduction machinery DOI Creative Commons
Leonid Digel, Mads L. Justesen, Nikoline S Madsen

et al.

EMBO Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Cable bacteria are centimeter-long multicellular conducting electricity through periplasmic conductive fibers (PCFs). Using single-strain enrichments of the genera Electrothrix and Electronema we systematically investigate variations similarities in morphology electrical properties across both genera. Electrical conductivity different PCFs spans three orders magnitude warranting further investigations plasticity their conduction machinery. electron microscopy elemental analyses, show that two cable have similar cell envelopes cell–cell junction ultrastructures. Iron, sulfur, nickel signals co-localized with PCFs, indicating key functional roles these elements. The organized as stranded rope-like structures composed multiple strands. Furthermore, report lamellae-like formed at junctions a core layer connecting to intriguing vesicle-like inner membrane invaginations below PCFs. Finally, using bioinformatic tools, identify cytochrome family predicted structural homology known multi-heme nanowire proteins from other electroactive microorganisms suggest cytochromes can play role extra- or intercellular bacteria.

Language: Английский

Citations

0
Cytochrome gene expression shifts in Geobacter sulfurreducens to maximize energy conservation in response to changes in redox conditions DOI Creative Commons
Ethan Howley, Rosa Krajmalnik‐Brown, César I. Torres

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115524 - 115524

Published: July 8, 2023

Language: Английский

Citations

10