Advances in Novel Nanomaterial-Based Optical Fiber Biosensors—A Review

Biosensors, Journal Year: 2022, Volume and Issue: 12(10), P. 843 - 843

Published: Oct. 8, 2022

This article presents a concise summary of current advancements in novel nanomaterial-based optical fiber biosensors. The beneficial and biological properties nanomaterials, such as nanoparticle size-dependent signal amplification, plasmon resonance, charge-transfer capabilities, are widely used biosensing applications. Due to the biocompatibility bioreceptor combination, nanomaterials enhance sensitivity, limit detection, specificity, response time sensing probes, well signal-to-noise ratio optic platforms. has established practical method for improving performance With aforementioned outstanding nanomaterial properties, development biosensors been efficiently promoted. paper reviews application numerous field provides brief explanation mechanism.

Language: Английский

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Controllable Assembly of a Quantum Dot-Based Aptasensor Guided by CRISPR/Cas12a for Direct Measurement of Circulating Tumor Cells in Human Blood

Nano Letters, Journal Year: 2024, Volume and Issue: 24(7), P. 2360 - 2368

Published: Feb. 12, 2024

Accurate and sensitive analysis of circulating tumor cells (CTCs) in human blood provides a non-invasive approach for the evaluation cancer metastasis early diagnosis. Herein, we demonstrate controllable assembly quantum dot (QD)-based aptasensor guided by CRISPR/Cas12a direct measurement CTCs blood. We introduce magnetic bead@activator/recognizer duplex core–shell structure to construct multifunctional platform capture detection blood, without need additional CTC release re-identification steps. Notably, introduction separation ensures that only target-induced free activator can initiate downstream catalysis, efficiently avoiding undesired catalysis triggered inappropriate recognition activator/recognizer crRNAs. This achieves high CTC-capture efficiency (82.72%) with limit 2 mL–1 holding great promise liquid biopsy cancers.

Language: Английский

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Graphene metasurface biosensor design for label-free peptide detection with machine learning optimization based on support vector regression with polynomial kernel

Diamond and Related Materials, Journal Year: 2025, Volume and Issue: unknown, P. 112053 - 112053

Published: Jan. 1, 2025

Language: Английский

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Portable optical fiber biosensors integrated with smartphone: technologies, applications, and challenges [Invited]

Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(3), P. 1630 - 1630

Published: Feb. 9, 2024

The increasing demand for individualized health monitoring and diagnostics has prompted considerable research into the integration of portable optical fiber biosensors integrated with smartphones. By capitalizing on benefits offered by fibers, these enable qualitative quantitative biosensing across a wide range applications. sensors smartphones, which possess advanced computational power versatile sensing capabilities, addresses need rapid solutions. This extensive evaluation thoroughly examines domain in conjunction including hardware complexities, approaches, methods. Additionally, it explores applications, physiological chemical biosensing. Furthermore, review provides an analysis challenges that have been identified this rapidly evolving area concludes relevant suggestions progression field.

Language: Английский

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Integration of Demethylation-Activated DNAzyme with a Single Quantum Dot Nanosensor for Sensitive Detection of O⁶-Methylguanine DNA Methyltransferase in Breast Tissues

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4487 - 4494

Published: March 7, 2024

O6-Methylguanine-DNA-methyltransferase (MGMT) is a demethylation protein that dynamically regulates the O6-methylguanine modification (O6 MeG), and dysregulated MGMT implicated in various malignant tumors. Herein, we integrate demethylation-activated DNAzyme with single quantum dot nanosensor to sensitively detect breast tissues. The presence of induces O6 MeG-caged restoration catalytic activity. activated then specifically cleaves ribonucleic acid site hairpin DNA expose toehold sequences. liberated sequence may act as primer trigger cyclic exponential amplification reaction for generation enormous signal strands bind Cy5/biotin-labeled probes form sandwich hybrids. assembly hybrids onto 605QD obtains 605QD-dsDNA-Cy5 nanostructures, inducing efficient FRET between donor Cy5 acceptor. Notably, introduction mismatched base can greatly minimize background improve signal-to-noise ratio. This achieves dynamic range 1.0 × 10–8 0.1 ng/μL detection limit 155.78 aM, it screen inhibitors monitor cellular activity single-cell sensitivity. Moreover, distinguish level tissues cancer patients healthy persons, holding great potential clinical diagnostics epigenetic research studies.

Language: Английский

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Entropy-Driven Self-Assembly of Single Quantum Dot Sensor for Catalytic Imaging of Telomerase in Living Cells

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(51), P. 18092 - 18098

Published: Dec. 15, 2022

Telomerase is a highly valuable cancer diagnosis biomarker and promising therapy target. So far, most telomerase assays are limited by the involvement of tedious procedures, multiple enzymes, complicated reaction schemes. Sensitive monitoring low-abundant in living cells remains challenge. Herein, we demonstrate an entropy-driven catalytic assembly quantum dot (QD) sensors for accurate detection imaging activity cells. In this sensor, target specifically catalyzes extension primer, extended primer subsequently acts as catalyst to continuously initiate reaction, generating large number fluorophore- biotin-labeled DNAs that can be self-assembled on QD surface induce efficient Föster resonance energy transfer signal. The proposed sensor requires single step both recognition amplification signal, eliminating use either protein enzymes or laborious procedures. Taking advantage inherent superiority single-molecule fluorescence high efficiency demonstrates single-cell sensitivity vitro assay. Moreover, it capable screening inhibitor, discriminating different tumor from normal ones, even real-time cells, providing novel platform telomerase-associated drug screening.

Language: Английский

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Microfluidic paper-based analytical devices for cancer diagnosis

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 379, P. 133243 - 133243

Published: Dec. 28, 2022

Language: Английский

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Metal Nanoclusters in Point-of-Care Sensing and Biosensing Applications

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(14), P. 12609 - 12672

Published: July 19, 2023

Nanoclusters are nanostructures consisting of few to tens atoms which have attracted great interest from the scientific community due their applications in a myriad fields, catalysis biomedical applications. Due discrete energy levels resulting ultrasmall size, nanoclusters exhibit distinctly different chemical, optical, and electrical properties compared with larger nanoparticle counterparts. Unlike previously reported dimensions (1–10 nm), typically single advantage (for example, high conductivity or optical properties), can be used as signaling and/or reaction-accelerating materials almost all sensing assays enhanced properties. among most promising luminescent emitters (electrochemiluminescent, fluorescent, chemiluminescent, colorimetry) quantum yield better biocompatibility than conventional emitters. Considering excelent biocompatibility, tailored modification, desirable electrochemical tunability, opened new horizons designing high-performance biosensing devices. In addition, they represent excellent photoelectrochemical behaviors improved functionality environmentally friendly traditional compounds. This review discusses recent advances nanoclusters, biosensing, current limitations, prospects overcome these challenges. The present study gives much brighter vision that one by particular advantages role developing frameworks.

Language: Английский

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Assays for alkaline phosphatase that use L-ascorbic acid 2-phosphate as a substrate

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 495, P. 215370 - 215370

Published: Aug. 11, 2023

Language: Английский

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Construction of a Quantum-Dot-Based FRET Nanosensor through Direct Encoding of Streptavidin-Binding RNA Aptamers for N6-Methyladenosine Demethylase Detection

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(35), P. 13201 - 13210

Published: Aug. 21, 2023

N6-Methyladenosine (m6A) demethylases can catalyze the removal of methyl modification on m6A, and it is closely associated with occurrence, proliferation, differentiation, metastasis malignancies. The m6A (e.g., fat mass obesity-associated protein (FTO)) may act as a cancer biomarker are crucial for anticancer drug screening early clinical diagnosis. Herein, we demonstrate construction quantum-dot-based Förster resonance energy-transfer (FRET) nanosensor through direct encoding streptavidin-binding RNA aptamers (SA aptamers) demethylase detection. This employs multiple Cy5-molecule-labeled SA building materials to construct 605QD-RNA-Cy5 nanoassembly, exploits hinder effect upon elongation ligation reactions distinguish m6A-containing probes from demethylated probes. When present, generate probes, initiating strand extension yield complete transcription template aptamers. Subsequently, T7-assisted cascade amplification reaction activated transcribe abundant incorporation Cy5 fluorophores. Cy5-incorporated self-assembly onto streptavidin-coated 605QD surface obtain 605QD-SA aptamer-Cy5 nanoassemblies, resulting in generation distinct FRET signals. exhibits ultrahigh sensitivity excellent specificity, detect endogenous FTO at single-cell level. Furthermore, this precisely measure enzyme kinetic parameters, screen inhibitors, differentiate expression between breast patients healthy individual tissues, offering versatile platform diagnostic discovery.

Language: Английский

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