Chemistry - A European Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

α-Cyanostilbene represents one of the easily functionalized aggregation-induced emission (AIE) scaffolds. It has been widely adopted for construction fluorescent materials broad applications. Here, we further expanded utilization α-cyanostilbene derivatives detection hypoxia or proteostasis imbalance in live cells. Four different amine containing donors were introduced to construct (R-ASC) with donor-acceptor Equipped cysteine (Cys) reactive group, maleimide (MI), R-ASC-MI shows fluorescence turn-on property upon binding unfolded proteins vitro and cells under proteostatic stress. By virtue R-ASC-MI, level protein loads can be quantified by flow cytometry, visualized microscope. Furthermore, also characterized performance R-ASC-NO

Language: Английский