Novel Endoplasmic Reticulum-Targeted Luminescent Probe for Visualization of Carbon Monoxide in Drug-Induced Liver Injury

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18246 - 18253

Published: Nov. 4, 2024

Drug-induced liver injury (DILI) is a major hepatic dysfunction commonly caused by hepatotoxic drug overdose, resulting in considerable number of fatalities worldwide. Recent studies have highlighted the regulatory and hepatoprotective effects carbon monoxide (CO) during process. However, precisely tracking dynamic changes composition CO DILI still great challenge. In this work, leveraging innovative "quencher-insertion" strategy, unique endoplasmic reticulum (ER)-targetable lanthanide complex-based luminescence probe, ER-ANBTTA-Eu3+/Tb3+, has been developed for selective accurate monitoring fluxes live cells laboratory animals. The new probe composed three covalently linked functional moieties: terpyridine polyacid-Eu3+/Tb3+-mixed chelates as long-lived luminophore, p-toluenesulfonamide moiety ER-anchoring motif, an allyloxy-nitrobenzyl ether CO-specific recognition unit. Upon reaction with presence Pd2+ ions, Tsuji–Trost leads to cleavage group from Eu3+/Tb3+-mixed chelates, which results restoration Tb3+ emission at 538 nm attenuation Eu3+ 688 nm, leading dramatic increase I538/I688 ratio. addition exceptional response sensitivity selectivity toward CO, ER-ANBTTA-Eu3+/Tb3+ also exhibits outstanding ER-locating capability, allows be used imaging ER cells. Using combined time-gated mode, exogenous endogenous were monitored without interference background autofluorescence. Moreover, upregulation mice was successfully visualized. suggested potential deeply exploring functions pathogenesis.

Language: Английский

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Recent advances in single fluorescent probes for monitoring dual organelles in two channels

Smart Molecules, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of organelle networks associated multiple pathological processes. However, clearly intuitively visualizing highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due distinct advantages facile, non‐invasiveness detection, making it particularly well‐suited applications in uncovering mysterious veil interactions. Regrettably, lack ideal fluorescence agents has always been an obstacle intricate behaviors organelles. In this review, we provide a systematic discussion on existing dual‐color dual‐targetable molecular sensors used monitoring interactions, focus their targeting strategies, mechanisms biological applications. Additionally, current limitations future development directions probes dual‐emissives briefly discussed. This review aims guidance researchers develop more improved studying biomedical field.

Language: Английский

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Next-generation Red Ultra-bright Fluorescent Dyes for Nuclear Imaging and Peripheral Blood Leukocytes Sorting

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The red ultra-bright nuclear-targeted dye BPC1 was developed. exhibits ultrahigh fluorescence brightness and exceptional cell permeability, enabling the visualization of nucleus by substantially lower doses (100 nM) laser power.

Language: Английский

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