Vanin-1-Activated Fluorescent Probe for Real-Time In Vivo Imaging of Inflammatory Responses Across Multiple Tissue Types

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Vanin-1 is a pantetheine hydrolase that plays key role in inflammatory diseases. Effective tools for noninvasive, real-time monitoring of are lacking, largely due to background fluorescence interference existing probes. To address this issue, we developed dual-modal fluorescent and colorimetric probe, MB-Van1, detect with high sensitivity selectivity. MB-Van1 has structure optimized exhibit nearly zero fluorescence, resulting signal-to-noise ratio enables the accurate detection activity various biological tissues. In vitro experiments demonstrated had limit as low 0.031 ng/mL mode. We successfully employ observe elevated levels tissues mouse models rheumatoid arthritis (RA), drug-induced liver injury (DILI), nonsteroidal anti-inflammatory drug (NSAID) enteropathy models, within only 5 min. This advancement provides novel approach dynamic changes during inflammation, offering new strategies early diagnosis therapeutic assessment other related

Language: Английский

---

A near-infrared fluorescence probe for selective detection of butyrylcholinesterase and its application in colitis and colorectal cancer diagnosis and therapeutic evaluation

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113014 - 113014

Published: Feb. 1, 2025

Language: Английский

---

Development of a Mitochondria-Targeted Ruthenium(II)-Based Phosphorescent Probe for Hypochlorite Detection in Acute Inflammatory Model

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

The uncontrolled acute inflammatory response triggers dysregulation of the immunoinflammatory system, contributing to development and progression various diseases (AIDs). Hypochlorite (ClO–), as a crucial oxidative mediator in AIDs, accumulates environment, leading direct cytotoxicity, secondary injury, tissue dysfunction. However, achieving rapid detection, accurate tracking, situ monitoring, real-time imaging ClO– vivo remains significant challenge. To address these issues, we developed mitochondria-targeted phosphorescent probe (RuDM), which introduces ligand containing C═N bond recognition site precisely identify AIDs. It responds rapidly (6 s) exhibits long-lived luminescence (471 ns), with 190-fold enhancement monitoring ClO–. Meanwhile, density functional theory (DFT) indicates that RuCOOH is attributed removal an electron-withdrawing group (diaminomaleonitrile) from RuDM, leads increase intersystem crossing rate greater probability radiative transition T1 state. Finally, RuDM used monitor levels exogenous endogenous cells using confocal microscopy evaluate its capability for detection over time model. above results suggest novel molecular platform detect ClO–, has potential practical tool research on pathogenesis diseases.

Language: Английский

---

Near-infrared frequency upconversion fluorescent probe for rapid and sensitive visual detection of sulfur dioxide

The Analyst, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Inflammation is a complex physiological response involving various cellular and molecular events. Sulfur dioxide (SO

Language: Английский

---