Microscopy methods for the in vivo study of nanoscale nuclear organization DOI Creative Commons

Nidhi Rani Lokesh,

Mark E. Pownall

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(01)

Published: Feb. 3, 2025

Eukaryotic genomes are highly compacted within the nucleus and organized into complex 3D structures across various genomic physical scales. Organization plays a key role in gene regulation, both facilitating regulatory interactions to promote transcription while also enabling silencing of other genes. Despite functional importance genome organization determining cell identity function, investigating nuclear this wide range scales has been challenging. Microscopy provides opportunity for direct visualization pioneered discoveries field. Nonetheless, nanoscale nucleus, such as nucleosomes chromatin loops, requires super-resolution imaging go beyond ~220 nm diffraction limit. Here, we review recent advances technology their promise uncover new insights at nanoscale. We discuss different modalities how they have applied with focus on light microscopy its application vivo systems. Finally, conclude our perspective continued technical innovations will advance understanding structure function.

Language: Английский

Microscopy methods for the in vivo study of nanoscale nuclear organization DOI Creative Commons

Nidhi Rani Lokesh,

Mark E. Pownall

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(01)

Published: Feb. 3, 2025

Eukaryotic genomes are highly compacted within the nucleus and organized into complex 3D structures across various genomic physical scales. Organization plays a key role in gene regulation, both facilitating regulatory interactions to promote transcription while also enabling silencing of other genes. Despite functional importance genome organization determining cell identity function, investigating nuclear this wide range scales has been challenging. Microscopy provides opportunity for direct visualization pioneered discoveries field. Nonetheless, nanoscale nucleus, such as nucleosomes chromatin loops, requires super-resolution imaging go beyond ~220 nm diffraction limit. Here, we review recent advances technology their promise uncover new insights at nanoscale. We discuss different modalities how they have applied with focus on light microscopy its application vivo systems. Finally, conclude our perspective continued technical innovations will advance understanding structure function.

Language: Английский

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