Integrative role of CTPS cytoophidia in polyploid tissue growth and nutrient adaptation DOI

Jiayi Hong,

Jiamin Li, Yuanbing Zhang

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Tissue growth and development are fundamental to organismal survival, requiring precise coordination of metabolic processes, nutrient availability, signaling pathways. Cytidine triphosphate synthase (CTPS) is a rate‐limiting enzyme in nucleotide biosynthesis assembles filamentous cytoophidia, conserved across species. Despite increasing interest how CTPS filaments integrate cues drive cell size tissue remains incompletely understood. Using RNA interference clustered regularly interspaces short palindromic repeats (CRISPR) / CRISPR‐associate nuclease 9 gene editing, we generated ‐knockdown point‐mutated mutants investigate the role cytoophidia growth. Specifically, introduced H355A mutation, which disrupts filament formation without affecting its enzymatic activity. Our findings revealed that depletion or disruption significantly impairs polyploid organs, such as fat body salivary glands, underscoring pivotal regulation. Mutants lacking exhibited reduced DNA replication activity smaller sizes compared wild‐type controls. Mechanistically, found nutrient‐sensing pathways, particularly insulin‐PI3K‐Akt pathway, regulate expression response availability. Activation sterol regulatory element‐binding protein partially rescued defects caused by depletion. These provide new insights into molecular mechanisms regulation filaments, highlighting their critical mediators integrating environmental demands, metabolism, pathways adaptation.

Language: Английский

Integrative role of CTPS cytoophidia in polyploid tissue growth and nutrient adaptation DOI

Jiayi Hong,

Jiamin Li, Yuanbing Zhang

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Tissue growth and development are fundamental to organismal survival, requiring precise coordination of metabolic processes, nutrient availability, signaling pathways. Cytidine triphosphate synthase (CTPS) is a rate‐limiting enzyme in nucleotide biosynthesis assembles filamentous cytoophidia, conserved across species. Despite increasing interest how CTPS filaments integrate cues drive cell size tissue remains incompletely understood. Using RNA interference clustered regularly interspaces short palindromic repeats (CRISPR) / CRISPR‐associate nuclease 9 gene editing, we generated ‐knockdown point‐mutated mutants investigate the role cytoophidia growth. Specifically, introduced H355A mutation, which disrupts filament formation without affecting its enzymatic activity. Our findings revealed that depletion or disruption significantly impairs polyploid organs, such as fat body salivary glands, underscoring pivotal regulation. Mutants lacking exhibited reduced DNA replication activity smaller sizes compared wild‐type controls. Mechanistically, found nutrient‐sensing pathways, particularly insulin‐PI3K‐Akt pathway, regulate expression response availability. Activation sterol regulatory element‐binding protein partially rescued defects caused by depletion. These provide new insights into molecular mechanisms regulation filaments, highlighting their critical mediators integrating environmental demands, metabolism, pathways adaptation.

Language: Английский

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