Extracellular Vesicle, Journal Year: 2024, Volume and Issue: 5, P. 100058 - 100058
Published: Dec. 17, 2024
Language: Английский
Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(4)
Published: April 1, 2024
Osteoporosis (OP) is a systematic bone disease characterized by low mass and fragile microarchitecture. Conventional treatment for OP has limited efficacy long-term toxicity. Synthetic biology makes bacterial extracellular vesicle (BEVs)-based therapeutic strategies promising alternative the of OP. Here, we constructed recombinant probiotics Escherichia coli Nissle 1917-pET28a-ClyA-BMP-2-CXCR4 (ECN-pClyA-BMP-2-CXCR4), in which BMP-2 CXCR4 were overexpressed fusion with BEVs surface protein ClyA. Subsequently, isolated engineered BEVs-BMP-2-CXCR4 (BEVs-BC) therapy. The BEVs-BC exhibited great targeting vivo. In addition, had good biocompatibility remarkable ability to promote osteogenic differentiation BMSCs. Finally, synthetic biology-based significantly prevented an ovariectomized (OVX) mouse model. conclusion, both bone-targeting bone-forming one-step using biology, provides effective strategy potential industrialization.
Language: Английский
Citations
38
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)
Published: May 21, 2024
The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development
Language: Английский
Citations
34
Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30
Published: April 23, 2024
Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.
Language: Английский
Citations
29
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159545 - 159545
Published: Jan. 13, 2025
Language: Английский
Citations
5
Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217
Published: Jan. 1, 2024
The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.
Language: Английский
Citations
16
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152469 - 152469
Published: May 22, 2024
Language: Английский
Citations
13
Journal of Advanced Research, Journal Year: 2024, Volume and Issue: 66, P. 329 - 347
Published: Jan. 11, 2024
Bone metabolism can maintain the normal homeostasis and function of bone tissue. Once balance is broken, it will cause osteoporosis, osteoarthritis, defects, tumors, or other diseases. However, such orthopedic diseases still have many limitations in clinical treatment, as drug restrictions, tolerance, side effects, implant rejection. review: In complex therapy regeneration, extracellular derivatives become a promising research focus to solve problems metabolic These derivatives, which include components matrix, growth factors, vesicles, significant therapeutic potential. It has advantages good biocompatibility, low immune response, dynamic demand for The purpose this review provide comprehensive perspective on elucidate intrinsic properties versatility derivatives. Further discussion them innovative advanced materials improving effectiveness regeneration processes. Key scientific concepts review, we first listed types functions three Then, discussed effects different cell sources metabolism. Subsequently, collected applications treatment summarized challenges applications. Finally, prospected novel We hope that understanding new solutions
Language: Английский
Citations
12
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Nov. 6, 2024
Organoids are "mini-organs" that self-organize and differentiate from stem cells under in vitro 3D culture conditions, mimicking the spatial structure function of tissues vivo. Extracellular vesicles (EVs) nanoscale phospholipid bilayer secreted by living cells, rich bioactive molecules, with excellent biocompatibility low immunogenicity. Compared to EVs, organoid-derived EVs (OEVs) exhibit higher yield enhanced biological functions. possess cell characteristics, OEVs capable delivering active substances, making both highly promising for medical applications. In this review, we provide an overview fundamental principles organoids OEVs, discuss their current applications disease treatment. We then focus on differences between traditional EVs. Subsequently, present methods engineering modification OEVs. Finally, critically summarize advantages challenges conclusion, believe a deeper understanding will innovative solutions complex diseases.
Language: Английский
Citations
10
Applied Materials Today, Journal Year: 2024, Volume and Issue: 36, P. 102078 - 102078
Published: Jan. 20, 2024
Language: Английский
Citations
9
Nano Research, Journal Year: 2023, Volume and Issue: 17(4), P. 2857 - 2873
Published: Sept. 18, 2023
Language: Английский
Citations
17