In
this
study,
we
explored
different
bacterial
strains
(Clostridium
beijerinckii,
C.
acetobutylicum,
oryzae,
and
kainantoi)
belonging
to
the
Clostridium
group
produced
butyric
acid
(C4)
using
acetate
as
a
carbon
source.
All
significant
amounts
of
C4,
but
beijerinckii
1.54
g/L
which
is
almost
equivalent
production
capacity
(1.63
g/L)
kluyveri.
Further
experiments
were
performed
diluted
raw
cheese
whey
(CW)
by
inoculating
mixed
cultures
containing
Clostridia,
Bacillus,
Desulfobacteraceae
groups.
kluyveri
was
added
culture,
it
stimulated
caproic
(C6)
production.
Mixed
culture
13.97
g/L,
10.83
6.81
C6
when
incubated
with
two
times,
five
ten
times
CW,
respectively,
within
20-day
incubation
period.
Compared
our
previous
higher
faster.
These
results
indicated
dilution
ratio
CW
an
important
factor
in
facilitating
production,
fatty
acids
are
than
that
single
i.e.,
Results
have
depicted
potential
employing
bio-augmentation
strategy
for
valorization
bioresources
into
valuable
products
like
acid.
Chemosphere,
Journal Year:
2024,
Volume and Issue:
356, P. 141950 - 141950
Published: April 8, 2024
Due
to
their
excellent
properties,
polyhydroxyalkanoates
are
gaining
increasing
recognition
in
the
biodegradable
polymer
market.
These
biogenic
polyesters
characterized
by
high
biodegradability
multiple
environments,
overcoming
limitation
of
composting
plants
only
and
versatility
production.
The
most
consolidated
techniques
literature
or
reference
legislation
for
physical,
chemical
mechanical
characterisation
final
product
reported
since
its
usability
on
market
is
still
linked
quality,
including
certificate.
This
makes
a
promising
prospect
with
potential
replace
fossil-based
thermoplastics
sustainably.
review
analyses
compares
properties
poly-β-hydroxybutyrate
poly-β-hydroxybutyrate-co-β-hydroxyvalerate,
indicating
current
limitations
strengths.
In
particular,
copolymer
characterised
better
performance
terms
crystallinity,
hardness
workability.
However,
knowledge
this
area
infancy,
selling
prices
too
(9
18
$
kg-1).
An
analysis
main
extraction
techniques,
established
development,
also
included.
Solvent
currently
widely
used
method
due
efficiency
quality.
context,
phase
biopolymer
production
process
remains
major
challenge
costs
need
use
non-halogenated
toxic
solvents
improve
good-quality
bioplastics.
discusses
all
fundamental
parameters
optimising
process,
such
as
solubility
temperature.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
927, P. 172138 - 172138
Published: April 4, 2024
Polyhydroxyalkanoates
(PHAs)
are
biobased
and
biodegradable
polymers
that
could
effectively
replace
fossil-based
non-biodegradable
plastics.
However,
their
production
is
currently
limited
by
the
high
costs,
mainly
due
to
costly
carbon
sources
used,
low
productivity
quality
of
materials
produced.
A
potential
solution
lies
in
utilizing
cheap
renewable
as
primary
feedstock
during
biological
PHAs,
paving
way
for
a
completely
sustainable
economically
viable
process.
In
this
review,
opportunities
challenges
related
polyhydroxyalkanoates
using
methane
volatile
fatty
acids
(VFAs)
substrates
were
explored,
with
focus
on
poly(3-hydroxybutyrate)
poly(3-hydroxybutyrate-co-3-hydroxyvalerate).
The
discussion
reports
current
knowledge
about
promising
Type
II
methanotrophs,
impact
process
parameters
such
limiting
nutrients,
CH4:O2
ratio
temperature,
type
co-substrate
its
concentration.
Additionally,
strategies
developed
until
now
enhance
PHA
yields
also
discussed.
Bioresource Technology,
Journal Year:
2023,
Volume and Issue:
387, P. 129699 - 129699
Published: Aug. 19, 2023
In
this
work,
the
potential
of
Methylocystis
hirsuta
to
simultaneously
use
methane
and
volatile
fatty
acids
mixtures
for
triggering
PHBV
accumulation
was
assessed
first
time
batchwise.
Biotic
controls
carried
out
with
CH4
alone
confirmed
inability
produce
achieved
71.2
±
7
g
m−3d−1
PHB.
Pure
valeric
acid
two
synthetic
simulating
VFAs
effluents
from
anaerobic
digestion
food
waste
at
35
°C
(M1)
55
(M2)
were
supplied
promote
3-HV
inclusion.
Results
showed
that
pure
supported
highest
polymer
yields
105.8
9
(3-HB:3-HV
=
70:30).
M1
led
a
maximum
103
4
85:15),
while
M2
mixtures,
which
did
not
include
acid,
no
PHV
synthesis.
This
suggested
synthesis
depends
on
composition
can
be
tuned
during
process.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
929, P. 172599 - 172599
Published: April 22, 2024
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
is
a
biobased
and
biodegradable
polymer
that
could
efficiently
replace
fossil-based
plastics.
However,
its
widespread
deployment
slowed
down
by
the
high
production
cost.
In
this
work,
techno-economic
assessment
of
process
for
producing
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
from
low-cost
substrates,
such
as
methane
valeric
acid
derived
anaerobic
digestion
organic
wastes,
proposed.
Several
strategies
cost
abatement,
use
mixed
consortium
line
reagent
recycling
during
downstream,
were
adopted.
Different
scenarios
in
terms
production,
100
to
100,000
t/y,
analysed,
and,
each
case,
effect
reactor
volume
(small,
medium
large
size)
on
selling
price
was
assessed.
addition,
biomass
concentration
also
considered.
Results
show
minimum
plant
with
t/y
capacity,
accounting
18.4
€/kg,
highly
influenced
since
it
can
be
reduced
up
8.6
€/kg
increasing
total
suspended
solids
5
30
g/L,
This
adjustment
aligns
breakeven
point
PHBV
reported
average
commercial
price.
