TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118122 - 118122
Published: Dec. 1, 2024
Language: Английский
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 3, 2025
Development of a multiplexed and sensitive biosensing platform is priority for public health security. We report micropore resistance counting based on polystyrene microsphere size-based encoding Clostridium butyricum Argonaute (CbAgo) decoding ultrasensitive detection. Initially, we constructed target DNA-modified coding system counting. Subsequently, the precise recognition cleavage capabilities guide DNA-activated CbAgo protein enable encoded system. Changes in concentration microspheres are presented as signal readout. The demonstrated excellent performance detection three mycotoxins (with sensitivity range over 4 orders magnitude reaching pg/mL level) two inflammatory markers at pg/mL. Combining enzyme by with counting, developed highly tool wide-ranging potential applications such clinical diagnosis, food safety inspection, environmental monitoring.
Language: Английский
Citations
2
ACS Sensors, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Pathogenic bacterial infections pose a significant threat to human life, health, and socioeconomic development, with those arising from Escherichia coli (E. coli) O157:H7 being particularly concerning. Herein, customized aggregation-induced emission luminogens (AIEgen)-based signaling tags (TPA-galactose) were combined microfluidic chip for the determination of E. O157:H7. TPA-galactose undergoes hydrolysis by β-galactosidase, resulting in formation highly fluorescent TPA–OH AIE characteristics. Phages covalently bound surface magnetic beads specifically capture lyse O157:H7, releasing endogenous fluorescence intensity facilitates The process achieves sensitivity 45 CFU/mL min, requiring no DNA extraction or amplification, utilizing minimal sample volume, enabling accurate one-stop quantification live This strategy enables rapid on-site environmental, food, clinical samples, significantly enhancing public health safety.
Language: Английский
Citations
1
ACS Sensors, Journal Year: 2024, Volume and Issue: 9(11), P. 5665 - 5682
Published: Nov. 11, 2024
Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.
Language: Английский
Citations
6
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118130 - 118130
Published: Jan. 1, 2025
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141321 - 141321
Published: Feb. 19, 2025
Language: Английский
Citations
0
Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 23
Published: Oct. 21, 2024
Pathogens contamination is a pressing global public issue that has garnered significant attention worldwide, especially in light of recent outbreaks foodborne illnesses. Programmable nucleases like CRISPR/Cas and Argonaute hold promise as tools for nucleic acid testing owning to programmability the precise target sequence specificity, which been utilized development pathogens detection. At present, fluorescence, main signal output method, provides simple response mode sensing analysis. However, dependence fluorescence on large instruments correct analysis data limited its use remote areas. Lateral flow strips (LFS), emerging novel flexible substrate, offer plethora advantages, encompassing easy-to-use, rapidity, visualization, low-cost, portability, etc. The integration with LFS, lateral assay (LFA), rendered new on-site In review, we introduced two programmable Argonaute, followed by structure, principle advantages LFA. Then diversified engineering detection pattens viruses, bacteria, parasites, fungi based were summarized. Finally, challenge perspectives involved diagnostic assays discussed.
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118081 - 118081
Published: Nov. 1, 2024
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118122 - 118122
Published: Dec. 1, 2024
Language: Английский
Citations
1