Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 69, P. 106632 - 106632
Published: Nov. 30, 2024
Language: Английский
Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 69, P. 106632 - 106632
Published: Nov. 30, 2024
Language: Английский
ACS ES&T Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Language: Английский
Citations
1Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
The bioaugmentation performance is severely reduced in the treatment of high-saline pesticide wastewater because growth and degradation activity degraders are significantly inhibited by high salt concentrations. In this study, a heterologous biodegradation pathway comprising seven genes mpd/pnpABCDEF responsible for bioconversion p-nitrophenol (PNP)-substituted organophosphorus pesticides (OPs) into β-oxoadipate encoding Vitreoscilla hemoglobin (VHb) green fluorescent protein (GFP) were integrated genome salt-tolerant chassis Halomonas cupida J9, to generate genetically engineered halotolerant degrader J9U-MP. RT-PCR assays demonstrated that nine exogenous successfully transcribed mRNA Gas chromatography analysis methyl parathion (MP) its intermediates expressed MP hydrolase PNP-degrading enzymes PnpABCD show obvious toward specific substrates Stable isotope showed J9U-MP able efficiently convert 13C6-PNP 13CO2, demonstrating complete mineralization high-salt media. stable during passage culture, genomic integration does not negatively influence Under oxygen-limited conditions, VHb-expressing inhibition significant reduction rate. A real-time monitoring system with enhanced GFP used track motion bioremediation. Moreover, 50 mg/L (i.e., PNP HQ) completely degraded within 12 h supplemented 60 g/L NaCl. After 3 days incubation, 25 was converted 13CO2 Our results highlight power synthetic biology creating new pollutant-mineralizing strains. strong competitive advantages high-salinity low-oxygen environments make suitable situ OP wastewater.
Language: Английский
Citations
0Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 209, P. 106060 - 106060
Published: March 28, 2025
Language: Английский
Citations
0Journal of Environmental Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0Heliyon, Journal Year: 2024, Volume and Issue: unknown, P. e37384 - e37384
Published: Sept. 1, 2024
Language: Английский
Citations
1Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 69, P. 106632 - 106632
Published: Nov. 30, 2024
Language: Английский
Citations
0