Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158848 - 158848
Published: Dec. 1, 2024
Language: Английский
Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119626 - 119626
Published: Sept. 19, 2024
Language: Английский
Citations
6
Preparative Biochemistry & Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18
Published: Jan. 21, 2025
Halophilic bacteria are promising candidates for biofuel production because of their efficient cellulose degradation. Their cellulases exhibit high activity, even in the presence inhibitors and under extreme conditions, making them ideal biorefinery applications. In this study, we isolated a strain Halomonas elongata (Kadal6) from decomposed cotton cloth on Rameshwaram seashore. Morphological, biochemical, 16S rRNA analyses revealed that Kadal6 was 99.93% similar to cellulase-producing strain, H. MH25661. The tolerance cellulase assessed through molecular docking with model MH25661 generated by I-TASSER experimentally using response surface methodology (RSM) Kadal6. A study indicated inhibition constant ethanol, hydroxymethylfurfural (HMF), furfural. Cellulase (CellHe) showed maximum rate 44.27% at 55 °C, 15% 6.5 g/L furfural HMF. enzyme retained 50% its activity these inhibitors, remained unaffected 1 HMF, although occurred 3 g/L. demonstrated significant both vitro silico, indicating potential applications harsh environments.
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
The plasma-activated water (PAW) containing numerous reactive species can facilitate chitin degradation. Given the intricate interplay between PAW treatment and diverse activities of chitinolytic enzymes, further investigation is imperative for enhancing bioconversion efficiency. This study revealed that PAW-treated exhibited improved degradability toward BtLPMO10A, endochitinases OfChtI, OfChtII-B4C1, exochitinase OfChi-h. Furthermore, H2O2 in boosted whereas soluble constituents generated during pretreatment inhibited Notably, this inhibition effect OfChi-h be mitigated by addition β-N-acetylhexosaminidase. In end, synergy among enzyme was also promoted pretreatment. On as a basis, degradation strategy using combination an cocktail applied to degrade chitin, achieving conversion yield 97% within 2 h. could other polysaccharides, such cellulose.
Language: Английский
Citations
0
Food Research International, Journal Year: 2025, Volume and Issue: 207, P. 116069 - 116069
Published: March 1, 2025
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
The conversion of cellulosic biomass into fermentable sugars represents a pivotal stage in the environmentally friendly and sustainable production clean fuels chemicals. In this paper, new strategy to enhance enzyme performance was proposed. This can achieve pH-sensitive recovery cellulase while improving catalytic activity stability enzyme. achieved by means chemical modification cysteine residue (−SH) carbohydrate-binding domain (CBD) with PEG-based modifier (Mal-PEG-FA). It shown that altered conformation cellulase, which significantly improved efficiency CBD enzyme, turn promoted particular, modified molecular weight 10 k demonstrated highest level activity, an increase 130.02% comparison native cellulase. Ultimately, effective aqueous phase system accomplished adjusting pH system. After five cycles, still maintained 81.0% its initial enzymatic activity. research is significant strategic importance for promotion efficient utilization.
Language: Английский
Citations
0
Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 229, P. 121028 - 121028
Published: April 22, 2025
Language: Английский
Citations
0
Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 230, P. 121091 - 121091
Published: April 27, 2025
Language: Английский
Citations
0
Separation Science Plus, Journal Year: 2024, Volume and Issue: 7(11)
Published: Sept. 10, 2024
ABSTRACT Honeysuckle, a traditional Chinese medicine, holds significant medicinal value, making it ubiquitous in the medical field. However, efficient extraction of chlorogenic acid from herbal medicine while maintaining its pharmacological activity remains pressing challenge. To address issues environmental pollution and inefficient using solvents, there is need to develop novel solvent. Due their low cost, nontoxic, environmentally friendly characteristics, deep eutectic solvents can be used for extracting materials preserving active ingredients during process. In this study, we examined antibacterial acid, key component honeysuckle, after We gained preliminary insights into extract against various bacteria, including Escherichia coli , Staphylococcus aureus Pseudomonas aeruginosa . Furthermore, analyzed extracts different concentrations liquid medium, morphology bacteria inhibition was analyzed. These findings suggest that solvent (DES) an efficient, friendly, cost‐effective method components honeysuckle. Moreover, extracted DES retain robust physiological activity.
Language: Английский
Citations
3
New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(36), P. 16015 - 16025
Published: Jan. 1, 2024
Choline chloride–acetic acid (1 : 2) composed DES has shown to be effective for the pretreatment of lignocellulosic biomass Miscanthus, increasing enzymatic susceptibility cellulose and quantitative conversion xylan fraction.
Language: Английский
Citations
2
Fuel, Journal Year: 2024, Volume and Issue: 379, P. 133053 - 133053
Published: Sept. 11, 2024
Language: Английский
Citations
2