European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 987, P. 177199 - 177199
Published: Dec. 10, 2024
Language: Английский
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 9, 2025
Early and precise diagnosis of neurodegenerative disorders like Alzheimer's (AD) Parkinson's (PD) is crucial for slowing their progression enhancing patient outcomes. Exosomal microRNAs (miRNAs) are emerging as promising biomarkers due to ability reflect the diseases' pathology, yet low abundance poses significant detection hurdles. This review article delves into burgeoning field electrochemical biosensors, designed exosomal miRNA biomarkers. Electrochemical biosensors offer a compelling solution, combining sensitivity required detect low-abundance with specificity needed discern profiles distinctive neural pathological states. We explore operational principles these including transduction mechanisms that facilitate detection. The also summarizes advancements in nanotechnology, signal enhancement, bioreceptor anchoring, microfluidic integration improve sensor accuracy. evidence use disease analyzed, focusing on clinical impact, diagnostic precision, obstacles faced practical applications. Their potential point-of-care testing regulatory considerations market entry discussed. Looking toward future, highlights forthcoming innovations might revolutionize early processes. impressive sensitivity, specificity, compatibility, track become instrumental diseases, possibly transforming care prognosis.
Language: Английский
Citations
1
Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 25, 2025
Language: Английский
Citations
0
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160850 - 160850
Published: Feb. 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Small extracellular vesicles (sEVs) are ideal biomarkers for early diagnosis of tumors, but their low density and small size make them difficult to enrich detect in body fluid samples. This study combined dielectrophoresis (DEP) with immunoaffinity methods proposed a novel, fast, efficient acDEP-sEV immunochip, which by filling the chip reaction chamber antibody-conjugated microbeads, nonuniform electric field can be constructed, laminar flow disrupted, mass transfer efficiency improved, fluorescence signals focused, ultimately achieving rapid, sensitive, adjustable sEVs capture detection. method only requires 20–50 μL plasma sample liquid biopsy less than 35 min. We analyzed total sEVs, EpCAM, MUC1 positive clinical samples, found that evaluation multiple has extremely high sensitivity, accuracy, specificity. Meanwhile, also achieved limit detection 54 sEV/μL. hybrid approach provides new strategy rapid sensitive cancer other diseases based on sEVs.
Language: Английский
Citations
0
Electrophoresis, Journal Year: 2025, Volume and Issue: unknown
Published: March 6, 2025
Exosomes, which constitute a subclass of extracellular vesicles found in various body fluids, play crucial role intercellular communication. Exosomes consist matter derived from their host, such as nucleic acids, proteins, and membrane proteins. Cancer cell-derived exosomes have cancer-specific molecules are the fluids. As such, they highly useful liquid biopsies for cancer diagnosis. For diagnosis, rapid simple determination sampled is important. Dielectrophoresis (DEP) manipulation has potential use biopsies. DEP behavior based on variations not been well investigated. In this study, dielectrophoretic characteristics four cell lines were investigated: MCF-7, PC-3, HT-29, COLO 201. The crossover frequencies these determined by directly observing behaviors at suspension medium conductivities. Each exosome type exhibited distinct characteristics, with significant differences lower linked to properties inner conductivity. A single-shell dielectric model was used estimate physical parameters exosomes, aligning experimental results. These findings highlight specific separation, enabling exosome-based
Language: Английский
Citations
0
Electrophoresis, Journal Year: 2025, Volume and Issue: unknown
Published: March 18, 2025
ABSTRACT Accurate cancer cell analysis is critical across a wide range of medical fields, including clinical diagnostics, personalized medicine, drug development, and research. The ability to rapidly analyze characterize cells key understanding tumor characteristics, developing targeted therapies, improving patient outcomes. Microscale electrokinetic (EK) techniques have demonstrated their effectiveness reliability as powerful tools for analysis, cancerous cells. applications dielectrophoresis (DEP), an EK technique, in are discussed here with focus on carcinomas, that develops epithelial tissue. In this review article, the working mechanism DEP first introduced, followed by in‐depth recent studies published between 2015 2024. concluding remarks section provides summary points offers insights into potential future advancements DEP‐based systems analyzing
Language: Английский
Citations
0
Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 192, P. 110213 - 110213
Published: April 24, 2025
Language: Английский
Citations
0
The Analyst, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Microfluidic chip technology, an emerging interdisciplinary field, enables precise control of fluids at micro- and nano-scales is widely applied in biomedicine, chemical analysis, drug screening.
Language: Английский
Citations
0
Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 192, P. 328 - 339
Published: Nov. 24, 2024
Language: Английский
Citations
0
European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 987, P. 177199 - 177199
Published: Dec. 10, 2024
Language: Английский
Citations
0