Published: Jan. 1, 2024
Language: Английский
Advanced Composites and Hybrid Materials, Journal Year: 2025, Volume and Issue: 8(1)
Published: Jan. 17, 2025
Hepatitis B virus DNA (HBV-DNA) serves as a crucial biomarker for the detection of hepatitis virus, with variations in its concentration blood samples providing vital insights into patient infection and recovery status. We have developed an electrochemical biosensor tailored HBV-DNA detection, exhibiting high sensitivity, specificity, broad range. The utilizes composite material composed MXene multiwalled carbon nanotubes (MX/MWCNTs), prepared through hand-shaking self-assembly technique, to modify glassy electrode (GCE) serve signal receivers. A meticulously designed padlock probe specifically recognizes forms double-stranded structure, which is subsequently degraded by exonuclease III. resulting sequence then hybridizes PolyT-primer create template, triggering rolling circle amplification releasing substantial amount pyrophosphate initial level amplification. Subsequently, induces release methylene blue (MB) from MB@ZIF-90 composite, achieving second released MB adsorbed MX/MWNCNTs-modified GCE, possesses cationic dye adsorption capabilities large specific surface area, generating completing third This offers range spanning 1.0 pM × 106 pM, remarkable limit low 0.5 (3σ/S), capable distinguishing base mismatches. Furthermore, it has demonstrated excellent specificity performance serum testing. this demonstrates significant potential clinical application, ultimate goal improving diagnosis management B.
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118253 - 118253
Published: April 1, 2025
Language: Английский
Citations
0
Sensors, Journal Year: 2025, Volume and Issue: 25(8), P. 2508 - 2508
Published: April 16, 2025
Simultaneous detection of dopamine (DA) and uric acid (UA) is essential for diagnosing neurological metabolic diseases but hindered by overlapping electrochemical signals. We present an ultrasensitive sensor using copper nanoclusters anchored on nitrogen-doped crumpled Ti3C2Tx MXene (Cu-N/Ti3C2Tx). The engineered 3D architecture prevents restacking, exposes active sites, enhances ion transport, while Cu boost electrocatalytic activity via accelerated electron transfer. Structural analyses confirm uniform dispersion (3.0 wt%), Ti-N bonding, strain-induced wrinkles, synergistically improving conductivity. achieves exceptional sensitivity (1958.3 1152.7 μA·mM−1·cm−2 DA/UA), ultralow limits (0.058 0.099 μM rapid response (<1.5 s), interference resistance (e.g., ascorbic acid). Differential pulse voltammetry enables independent linear ranges (DA: 2–60 μM; UA: 5–100 μM) in biofluids, with 94.4% stability retention over 7 days. designed exhibits excellent capabilities DA UA detection. This work provides a novel design strategy developing high-performance sensors.
Language: Английский
Citations
0
Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128219 - 128219
Published: April 1, 2025
Language: Английский
Citations
0
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(45), P. 61692 - 61702
Published: Nov. 4, 2024
Exosomes have been perceived as promising biomarkers for noninvasive cancer diagnosis and treatment monitoring. However, the sensitive accurate quantification phenotyping of exosomes remains challenging. Herein, a versatile electrochemiluminescence (ECL) aptasensor was proposed analysis tumorous exosomes. Specifically, ternary nanohybrid (Ru-HAuTiO2), by covalently linking ECL luminophore Ru(dcbpy)32+ with gold nanoparticles (AuNPs)-decorated hollow urchin-like TiO2 (HTiO2), ingeniously designed highly luminescent self-enhanced nanoemitter. Notably, porous HTiO2 played an "all-rounder" role, including carrier luminophores AuNPs, coreaction accelerator, specific exosome capturing scaffold through Ti–phosphate coordination interaction. On other hand, polydopamine modified covalent organic framework (PDA@COF) employed quencher to remarkably attenuate Ru-HAuTiO2 dual-quenching mechanism, further labeled aptamer (Apt) exosomal surface protein. Based on forming Ru-HAuTiO2/exosome/Apt-PDA@COF sandwich structure electrode, "signal on–off" platform constructed, realizing detection within range 3.1 × 103 particles/mL 1 108 low limit 1.41 particles/mL, achieving phenotypic profiling proteins different This work provides alternative method
Language: Английский
Citations
3
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 155470 - 155470
Published: Sept. 3, 2024
Language: Английский
Citations
1
Chemical Physics Reviews, Journal Year: 2024, Volume and Issue: 5(4)
Published: Dec. 1, 2024
Metal nanoclusters (NCs) composed of metal atoms and ligands show promise as electrochemiluminescent (ECL) materials due to their optical electrochemical properties. This review provides a comprehensive overview the ECL mechanisms, advantages, roles NCs luminophores, catalysts, quenchers, substrates, carriers. Insights into factors that influence in will lay foundation for future research. In addition, applications metallic NC-based detection platforms are summarized, potential research directions presented.
Language: Английский
Citations
1
Published: Jan. 1, 2024
Language: Английский
Citations
0