Electron Transfer-Driven Nanozymes Boost Biosensor Sensitivity via a Synergistic Signal Amplification Strategy

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

The conventional gold nanoparticles (AuNPs) with insufficient brightness face substantial challenges in developing a sensitive lateral flow immunoassay (LFIA). Herein, multibranched manganese–gold (Mn–Au) (MnAuNPs) Au core–Mn shell nanostructure were synthesized by one-pot method. Mn of valence-rich and core high electron transfer efficiency endowed MnAuNPs oxidase-like activity, which oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) only transfer. Ox-TMB, was the oxidation product TMB, is an excellent photothermal agent. Furthermore, synergistic effect ox-TMB significantly enhanced conversion efficiency. has enabled highly quantitative detection. LFIA based on (cascade LFIA) achieved detection Escherichia coli O157:H7. entire process completed 25 min. limit cascade 239 CFU mL–1, 37.21-fold lower than that AuNPs-LFIA (8892 mL–1). recoveries 82.63–111.67%, coefficients variation 4.28–14.19%. Overall, this work suggests potential development broadens biosensing strategies for point-of-care testing.

Language: Английский

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Plasmonics-Enhanced Dual-Modal Colorimetric and Photothermal Lateral Flow Immunoassay Using Gold Nanocages

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Lateral flow immunoassays (LFIA) are widely recognized as cost-effective point-of-care diagnostic tools (POCT) for infectious disease diagnosis. Despite their widespread use, traditional colorimetric LFIAs, which rely on gold nanospheres (GNP), constrained by a limited sensitivity. To overcome this challenge, we have engineered nanocages (GNCs) with optimized core-to-shell morphologies, achieving significant amplification of both and photothermal LFIA readout signals. The distinctive morphology GNCs, featuring adjustable gap thicknesses, enables fine-tuning the localized surface plasmon resonance (LSPR) peak across broad spectral range from 600 to 1200 nm. Among GNC morphologies evaluated, (GNC-4), characterized its larger size maximal thickness, exhibited superior color brightness enhanced efficiency compared other GNP. performance GNC-4 enabled detection influenza A (H1N1), used model analyte, limit (LOD) 1.8 ng/mL via analysis 1.51 pg/mL using LFIA. Compared GNP-based detection, sensitivity GNC-4-based was 7-fold, while showed an improvement over 8000-fold. By incorporating portable smartphone-based platform, our dual-modal exhibits high sensitivity, practicality in detecting H1N1 spiked saliva samples, long-term stability five months, making it promising tool potential diagnosing pathogens.

Language: Английский

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Multifunctional metal-organic frameworks-mediated colorimetric/photothermal immunosensor for highly sensitivity detection of dibutyl phthalate

Food Chemistry, Journal Year: 2025, Volume and Issue: 472, P. 142928 - 142928

Published: Jan. 15, 2025

Language: Английский

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Reasonable Design of Aggregation-Induced Emission Luminogens for Rapid Detection of Cardiac Troponin I by Lateral Flow Immunoassay

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137635 - 137635

Published: March 1, 2025

Language: Английский

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Electrochemiluminescence Lateral Flow Immunosensor Using Luminol-Labeled Silver Nanoparticles for Highly Sensitive and Quan-titative Detection of Cardiac Troponin I

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128159 - 128159

Published: April 1, 2025

Language: Английский

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A cellulose paper decorated with gold(-silver) nanoparticles for SERS-based immunoassays

Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128170 - 128170

Published: April 21, 2025

Language: Английский

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Plasmonic-Enhanced Colorimetric Lateral Flow Immunoassays Using Bimetallic Silver-Coated Gold Nanostars

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 29, 2024

The colorimetric lateral flow immunoassay (cLFIA) has gained widespread attention as a point-of-care testing (POCT) technique due to its low cost, short analysis time, portability, and capability of being performed by unskilled operators with minimal requirement reagents. However, the analytical sensitivity conventional LFIA based on colloidal gold nanospheres limits their applications for sensitive detection trace amounts target analytes. In this study, we introduced novel plasmonic-enhanced (PE-cLFIA) platform featuring bimetallic silver-coated nanostars (BGNS) exceptional optical properties, leading ultrahigh visual color brightness. BGNS-based PE-cLFIA was successfully applied detect model analyte, low-calcium response V (LcrV), virulence protein factor found in

Language: Английский

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Dual-plasmonic eccentric nanostructure with prominent colorimetric and photothermal performance to detect zearalenone by dual signal immunochromatography assay

Talanta, Journal Year: 2024, Volume and Issue: 286, P. 127487 - 127487

Published: Dec. 29, 2024

Language: Английский

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Selective surface passivation of gold nanoparticles: A strategy for enhanced sensitivity in lateral flow immunoassay

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158571 - 158571

Published: Dec. 1, 2024

Language: Английский

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Bifunctional bovine serum albumin modification driven sensitivity-enhanced lateral flow immunoassay for small molecule hazards monitoring in food

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136915 - 136915

Published: Oct. 30, 2024

Language: Английский

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