Engineering Strategies Enabled Protocatechuic Acid Production from Lignin by Pseudomonas putida KT2440 DOI
Zhen Chen, He Liu,

Qiu-Jin Zong

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Bioconversion of lignin into fine aromatics offers new avenues for both valorization and biomass utilization. However, the heterogeneity lack an effective conversion route pose challenges in valorization. Herein, advanced engineering strategies Pseudomonas putida have been designed high-yield production valuable protocatechuic acid (PCA) from lignin-derived real hydrolysates. Blocking PCA degradation pathway facilitated H- G-type aromatics. The aromatic hydroxylation O-demethylation steps were identified as metabolic bottlenecks producing aromatics, respectively. Modulating expression genes encoding rate-limiting enzymes PobA VanAB successfully eliminated accumulation intermediates, 4-hydroxybenzoic vanillic acid, By integrating these beneficial gene modifications, chromosomal strain PCA1601 showed a remarkable performance PCA, reaching titer 22.7 mM molar yield 94.5%. Implementing fed-batch strategy further increased mixed yielding record 113.6 (17.5 g/L). Most importantly, showcased capacity with hydrolysate, ultimately 15.3 (2.4 g/L) PCA. engineered plasmid-free P. represents promising platform industrial bioproduction renewable lignin.

Language: Английский

Harnessing Aromatic Properties for Sustainable Bio-valorization of Lignin Derivatives into Flavonoids DOI Creative Commons
Siyu Zhu, Na Li, Zhihua Liu

et al.

Green Carbon, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Emerging biotechnological strategies advancing biological lignin valorization towards polyhydroxyalkanoates DOI
Haiyuan Jia, Tao Xu,

Chen Wang

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132278 - 132278

Published: Feb. 1, 2025

Language: Английский

Citations

0
Densification pretreatment with p-toluene sulfonic acid promotes comprehensive separation and utilization of whole components of corn stover DOI
Qianqian Yang, Ye Tian, Yin Cheong Cheng

et al.

Separation and Purification Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132207 - 132207

Published: Feb. 1, 2025

Language: Английский

Citations

0
Prospecting artificial microbial consortia toward lignin valorization DOI
Jingjing Hu, Bin Gao, Zhihua Liu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162375 - 162375

Published: April 1, 2025

Language: Английский

Citations

0
Lignin-Degrading Enzymes and the Potential of Pseudomonas putida as a Cell Factory for Lignin Degradation and Valorization DOI Creative Commons

Qing Zhou,

Annabel Fransen,

Johannes H. de Winde

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 935 - 935

Published: April 18, 2025

Efficient utilization of lignin, a complex polymer in plant cell walls, is one the key strategies for developing green and sustainable bioeconomy. However, bioconversion lignin poses significant challenge due to its recalcitrant nature. Microorganisms, particularly fungi bacteria, play crucial role biodegradation, using various enzymatic pathways. Among Pseudomonas putida considered promising host degradation valorization, robust flexible metabolism tolerance many noxious toxic compounds. This review explores mechanisms breakdown by microorganisms, with focus on P. putida’s metabolic versatility genetic engineering potential. By leveraging advanced tools pathway optimization, can be engineered efficiently convert into valuable bioproducts, offering solutions valorization industrial applications.

Language: Английский

Citations

0
One-pot bioconversion of lignin to 4-vinylphenol derivatives DOI

Ruo-Ying Liu,

Chen Wang,

Bing‐Zhi Li

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156286 - 156286

Published: Sept. 1, 2024

Language: Английский

Citations

1
Engineering Strategies Enabled Protocatechuic Acid Production from Lignin by Pseudomonas putida KT2440 DOI
Zhen Chen, He Liu,

Qiu-Jin Zong

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Bioconversion of lignin into fine aromatics offers new avenues for both valorization and biomass utilization. However, the heterogeneity lack an effective conversion route pose challenges in valorization. Herein, advanced engineering strategies Pseudomonas putida have been designed high-yield production valuable protocatechuic acid (PCA) from lignin-derived real hydrolysates. Blocking PCA degradation pathway facilitated H- G-type aromatics. The aromatic hydroxylation O-demethylation steps were identified as metabolic bottlenecks producing aromatics, respectively. Modulating expression genes encoding rate-limiting enzymes PobA VanAB successfully eliminated accumulation intermediates, 4-hydroxybenzoic vanillic acid, By integrating these beneficial gene modifications, chromosomal strain PCA1601 showed a remarkable performance PCA, reaching titer 22.7 mM molar yield 94.5%. Implementing fed-batch strategy further increased mixed yielding record 113.6 (17.5 g/L). Most importantly, showcased capacity with hydrolysate, ultimately 15.3 (2.4 g/L) PCA. engineered plasmid-free P. represents promising platform industrial bioproduction renewable lignin.

Language: Английский

Citations

1